Multiple Defects of Immune Cell Function in Mice with Disrupted Interferon-γ Genes

Dalton, Dyana K., Pitts-Meek, Sharon, Keshav, Satish, Figari, Irene S., Bradley, Allan, Stewart, Timothy A.·1993·Science

DOI 10.1126/science.8456300

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Abstract

Interferon-γ (IFN-γ) is a pleiotrophic cytokine with immunomodulatory effects on a variety of immune cells. Mice with a targeted disruption of the IFN-γ gene were generated. These mice developed normally and were healthy in the absence of pathogens. However, mice deficient in IFN-γ had impaired production of macrophage antimicrobial products and reduced expression of macrophage major histocompatibility complex class II antigens. IFN-γ-deficient mice were killed by a sublethal dose of the intracellular pathogen Mycobacterium bovis . Splenocytes exhibited uncontrolled proliferation in response to mitogen and alloantigen. After a mixed lymphocyte reaction, T cell cytolytic activity was enhanced against allogeneic target cells. Resting splenic natural killer cell activity was reduced in IFN-γ-deficient mice. Thus, IFN-γ is essential for the function of several cell types of the murine immune system.

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Authors: Dalton, D. K., Pitts-Meek, S., Keshav, S., Figari, I. S., Bradley, A., & Stewart, T. A.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1993-03-19
Volume: 259
Issue: 5102
Pages: 1739-1742
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.8456300
OpenAlex: W2089262366
Cited by: 1,727
References: 0
FWCI: 27.10
Type: journal article
Language: en
Open access: closed

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Multiple Defects of Immune Cell Function in Mice with Disrupted Interferon-γ Genes

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