CoChat
Sign Up
Cited by 344Hormonal and reproductive studiesMuscle Physiology and DisordersMuscle metabolism and nutrition

Testosterone-induced muscle hypertrophy is associated with an increase in satellite cell number in healthy, young men

Sinha-Hikim, Indrani, Roth, Stephen M., Lee, Martin I., Bhasin, Shalender·2003·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00370.2002
Open this paper in CoChat — free account

Abstract

Testosterone (T) supplementation in men induces muscle fiber hypertrophy. We hypothesized that T-induced increase in muscle fiber size is associated with a dose-dependent increase in satellite cell number. We quantitated satellite cell and myonuclear number by using direct counting and spatial orientation methods in biopsies of vastus lateralis obtained at baseline and after 20 wk of treatment with a gonadotropin-releasing hormone agonist and a 125-, 300-, or 600-mg weekly dose of T enanthate. T administration was associated with a significant increase in myonuclear number in men receiving 300- and 600-mg doses. The posttreatment percent satellite cell number, obtained by direct counting, differed significantly among the three groups (ANCOVA P < 0.000001); the mean posttreatment values (5.0 and 15.0%) in men treated with 300- and 600-mg doses were greater than baseline (2.5 and 2.5%, respectively, P < 0.05 vs. baseline). The absolute satellite cell number measured by spatial orientation at 20 wk (1.5 and 4.0/mm) was significantly greater than baseline (0.3 and 0.6/mm) in men receiving the 300- and 600-mg doses ( P < 0.05). The change in percent satellite cell number correlated with changes in total ( r = 0.548) and free T concentrations ( r = 0.468). Satellite cell and mitochondrial areas were significantly higher and the nuclear-to-cytoplasmic ratio lower after treatment with 300- and 600-mg doses. We conclude that T-induced muscle fiber hypertrophy is associated with an increase in satellite cell number, a proportionate increase in myonuclear number, and changes in satellite cell ultrastructure.

Integrity signals

Registry

Crossref · 10.1152/ajpendo.00370.2002

Cites

58 of 58 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

1 of the 59 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Sinha-Hikim, I., Roth, S. M., Lee, M. I., & Bhasin, S.
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2003-07
Volume
285
Issue
1
Pages
E197-E205
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00370.2002
OpenAlex
W2105071743
Cited by
344
References
59
Citations by year
2026: 7 · 2025: 12 · 2024: 11 · 2023: 11 · 2022: 10 · 2021: 13 · 2020: 22 · 2019: 15 · 2018: 15 · 2017: 11 · 2016: 16 · 2015: 20 · 2014: 12 · 2013: 17 · 2012: 16
FWCI
7.94
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5u54rr014616-10, 5u01dk054047-06)
  • National Institute on Aging (1R01 AG-14369-01, 5F32 AG-05893-02, R01AG014369, F32AG005893)
  • National Institute of Diabetes and Digestive and Kidney Diseases (1R01 DK 59627-01, U01-DK-54047, R01DK049296, 2R01DK49296-02A, R01DK059627)
  • National Center for Research Resources (G12 RR 03026, P20RR11145, U54-RR14616, G12RR003026, P20RR011145, M01RR000425, M01-RR00425)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
testosterone, muscle hypertrophy, satellite cells, myonuclear number, testosterone enanthate, dose-response relationship, GnRH agonist, vastus lateralis, free testosterone, nuclear-cytoplasmic ratio, young men
MeSH terms
Adult, Cell Count, Cell Nucleus, Double-Blind Method, Humans, Gonadotropin-Releasing Hormone, Male, Microscopy, Electron, Testosterone, Tissue Fixation, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Satellite Cells, Skeletal Muscle, Cell Size

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review