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microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder

Perkins, Diana O, Jeffries, Clark D, Jarskog, L Fredrik, Thomson, J Michael, Woods, Keith, Newman, Martin A, Parker, Joel S, Jin, Jianping +1 more·2007·Genome Biology
DOI 10.1186/gb-2007-8-2-r27PMC PMC1852419
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Abstract

Background: microRNAs (miRNAs) are small, noncoding RNA molecules that are now thought to regulate the expression of many mRNAs. They have been implicated in the etiology of a variety of complex diseases, including Tourette's syndrome, Fragile × syndrome, and several types of cancer. Results: We hypothesized that schizophrenia might be associated with altered miRNA profiles. To investigate this possibility we compared the expression of 264 human miRNAs from postmortem prefrontal cortex tissue of individuals with schizophrenia (n = 13) or schizoaffective disorder (n = 2) to tissue of 21 psychiatrically unaffected individuals using a custom miRNA microarray. Allowing a 5% false discovery rate, we found that 16 miRNAs were differentially expressed in prefrontal cortex of patient subjects, with 15 expressed at lower levels (fold change 0.63 to 0.89) and 1 at a higher level (fold change 1.77) than in the psychiatrically unaffected comparison subjects. The expression levels of 12 selected miRNAs were also determined by quantitative RT-PCR in our lab. For the eight miRNAs distinguished by being expressed at lower microarray levels in schizophrenia samples versus comparison samples, seven were also expressed at lower levels with quantitative RT-PCR. Conclusion: This study is the first to find altered miRNA profiles in postmortem prefrontal cortex from schizophrenia patients.

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  • Altered miRNA profiles have been reported in the prefrontal cortex of patients with schizophrenia and schizoaffective disorder.

    “while another study has reported altered miRNA profiles in the prefrontal cortex of patients with schizophrenia and schizoaffective disorder (Perkins et al. 2007).”

    From MicroRNA expression in the adult mouse central nervous system (2008) · cites this paper as Perkins et al. 2007

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Cited by

Sorted by citations

Authors
Perkins, D. O., Jeffries, C. D., Jarskog, L. F., Thomson, J. M., Woods, K., Newman, M. A., Parker, J. S., Jin, J., & Hammond, S. M.
Venue
Genome Biology
Publisher
Springer Science and Business Media LLC
Published
2007-02-27
Volume
8
Issue
2
ISSN
1474-760X
DOI
10.1186/gb-2007-8-2-r27
PMC
PMC1852419
Cited by
479
References
6
Language
en
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