The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
NG2 cells generate both oligodendrocytes and gray matter astrocytes
Abstract
NG2 glia constitute a fourth major glial cell type in the mammalian central nervous system (CNS) that is distinct from other cell types. Although circumstantial evidence suggests that some NG2 glia differentiate into oligodendrocytes, their in vivo fate has not been directly examined. We have used the bacterial artificial chromosome (BAC) modification technique to generate transgenic mice that express DsRed or Cre specifically in NG2-expressing (NG2+) cells. In NG2DsRedBAC transgenic mice, DsRed was expressed specifically in NG2+ cells throughout the postnatal CNS. When the differentiation potential of NG2+ cells in vitro was examined using DsRed+NG2+cells purified from perinatal transgenic brains, the majority of the cells either remained as NG2+ cells or differentiated into oligodendrocytes. In addition, DsRed+NG2+ cells also differentiated into astrocytes. The in vivo fate of NG2 glia was examined in mice that were double transgenic for NG2creBAC and the Cre reporter Z/EG. In the double transgenic mice, the Cre reporter EGFP was detected in myelinating oligodendrocytes and in a subpopulation of protoplasmic astrocytes in the gray matter of ventrolateral forebrain but not in fibrous astrocytes of white matter. These observations suggest that NG2+ cells are precursors of oligodendrocytes and some protoplasmic astrocytes in gray matter.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kessaris, N., Fogarty, M., Iannarelli, P., Grist, M., Wegner, M., & Richardson, W. D. (2006) · Nature Neuroscience · doi:10.1038/nn1620 · Cited by 1,195
Dawson, M. (2003) · Molecular and Cellular Neuroscience · doi:10.1016/s1044-7431(03)00210-0 · Cited by 927
Yang, X. W., Model, P., & Heintz, N. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-859 · Cited by 544
McTigue, D. M., Wei, P., & Stokes, B. T. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-10-03392.2001 · Cited by 423
Differentiation and death of premyelinating oligodendrocytes in developing rodent brain
Trapp, B. D., Nishiyama, A., Cheng, D., & Macklin, W. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.2.459 · Cited by 395
Self-citationDifferentiation of proliferated NG2-positive glial progenitor cells in a remyelinating lesion
Watanabe, M., Toyama, Y., & Nishiyama, A. (2002) · Journal of Neuroscience Research · doi:10.1002/jnr.10338 · Cited by 261
Self-citationThe response of NG2-expressing oligodendrocyte progenitors to demyelination in MOG-EAE and MS
Reynolds, R., Dawson, M., Papadopoulos, D., Polito, A., di Bello, I. C., Pham-Dinh, D., & Levine, J. (2002) · Journal of Neurocytology · doi:10.1023/a:1025747832215 · Cited by 175
Cited by
Iliff, J. J., Wang, M., Liao, Y., Plogg, B. A., Peng, W., Gundersen, G. A., Benveniste, H., Vates, G. E., Deane, R., Goldman, S. A., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003748 · Cited by 5,824
Pericytes: Developmental, Physiological, and Pathological Perspectives, Problems, and Promises
Armulik, A., Genové, G., & Betsholtz, C. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.07.001 · Cited by 2,653
The glymphatic system: a beginner's guide
Jessen, N. A., Munk, A. S. F., Lundgaard, I., & Nedergaard, M. (2015) · Neurochemical Research · doi:10.1007/s11064-015-1581-6 · Cited by 2,075
Capillary pericytes regulate cerebral blood flow in health and disease
Hall, C. N., Reynell, C., Gesslein, B., Hamilton, N. B., Mishra, A., Sutherland, B. A., O’Farrell, F. M., Buchan, A. M., Lauritzen, M., & Attwell, D. (2014) · Nature · doi:10.1038/nature13165 · Cited by 1,870
Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
PDGFRA/NG2 glia generate myelinating oligodendrocytes and piriform projection neurons in adult mice
Rivers, L. E., Young, K. M., Rizzi, M., Jamen, F., Psachoulia, K., Wade, A., Kessaris, N., & Richardson, W. D. (2008) · Nature Neuroscience · doi:10.1038/nn.2220 · Cited by 961
Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity
Nishiyama, A., Komitova, M., Suzuki, R., & Zhu, X. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2495 · Cited by 865
Self-citationKang, S. H., Fukaya, M., Yang, J. K., Rothstein, J. D., & Bergles, D. E. (2010) · Neuron · doi:10.1016/j.neuron.2010.09.009 · Cited by 787
Self-citationZawadzka, M., Rivers, L. E., Fancy, S. P. J., Zhao, C., Tripathi, R., Jamen, F., Young, K., Goncharevich, A., Pohl, H., Rizzi, M., et al. (2010) · Cell stem cell · doi:10.1016/j.stem.2010.04.002 · Cited by 650
Heterogeneity of astrocytic form and function.
Oberheim, N. A., Goldman, S. A., & Nedergaard, M. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-452-0_3 · Cited by 638
Origin of New Glial Cells in Intact and Injured Adult Spinal Cord
Barnabé-Heider, F., Göritz, C., Sabelström, H., Takebayashi, H., Pfrieger, F. W., Meletis, K., & Frisén, J. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.07.014 · Cited by 628
Oligodendrocytes in development, myelin generation and beyond
Kuhn, S., Gritti, L., Crooks, D., & Dombrowski, Y. (2019) · Cells · doi:10.3390/cells8111424 · Cited by 625
Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease
Peng, W., Achariyar, T. M., Li, B., Liao, Y., Mestre, H., Hitomi, E., Regan, S., Kasper, T., Peng, S., Ding, F., et al. (2016) · Neurobiology of Disease · doi:10.1016/j.nbd.2016.05.015 · Cited by 580
Dimou, L., Simon, C., Kirchhoff, F., Takebayashi, H., & Götz, M. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.2831-08.2008 · Cited by 551
Local generation of glia is a major astrocyte source in postnatal cortex
Ge, W.-P., Miyawaki, A., Gage, F. H., Jan, Y. N., & Jan, L. Y. (2012) · Nature · doi:10.1038/nature10959 · Cited by 507
Single-cell CUT&Tag profiles histone modifications and transcription factors in complex tissues
Bartosovic, M., Kabbe, M., & Castelo-Branco, G. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00869-9 · Cited by 483
Lifelong cortical myelin plasticity and age-related degeneration in the live mammalian brain
Hill, R. A., Li, A. M., & Grutzendler, J. (2018) · Nature Neuroscience · doi:10.1038/s41593-018-0120-6 · Cited by 467
Age-dependent fate and lineage restriction of single NG2 cells
Zhu, X., Hill, R. A., Dietrich, D., Komitova, M., Suzuki, R., & Nishiyama, A. (2011) · Development · doi:10.1242/dev.047951 · Cited by 464
The life, death, and replacement of oligodendrocytes in the adult CNS
McTigue, D. M., & Tripathi, R. B. (2008) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2008.05570.x · Cited by 455
Stark, K., Eckart, A., Haidari, S., Tirniceriu, A., Lorenz, M., von Brühl, M.-L., Gärtner, F., Khandoga, A. G., Legate, K. R., Pless, R., et al. (2013) · Nature Immunology · doi:10.1038/ni.2477 · Cited by 447
Simon, C., Götz, M., & Dimou, L. (2011) · Glia · doi:10.1002/glia.21156 · Cited by 313
Astrocytes in multiple sclerosis: A product of their environment
Nair, A., Frederick, T. J., & Miller, S. D. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8059-5 · Cited by 313
Interpericyte tunnelling nanotubes regulate neurovascular coupling
Alarcon-Martinez, L., Villafranca-Baughman, D., Quintero, H., Kacerovsky, J. B., Dotigny, F., Murai, K. K., Prat, A., Drapeau, P., & Di Polo, A. (2020) · Nature · doi:10.1038/s41586-020-2589-x · Cited by 312
NG2-glia as multipotent neural stem cells: fact or fantasy?
Richardson, W. D., Young, K. M., Tripathi, R. B., & McKenzie, I. (2011) · Neuron · doi:10.1016/j.neuron.2011.05.013 · Cited by 300
Ma, Q., Zhao, Z., Sagare, A. P., Wu, Y., Wang, M., Owens, N. C., Verghese, P. B., Herz, J., Holtzman, D. M., & Zlokovic, B. V. (2018) · Molecular Neurodegeneration · doi:10.1186/s13024-018-0286-0 · Cited by 281
Excitability and synaptic communication within the oligodendrocyte lineage
De Biase, L. M., Nishiyama, A., & Bergles, D. E. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.6000-09.2010 · Cited by 286
Tripathi, R. B., Rivers, L. E., Young, K. M., Jamen, F., & Richardson, W. D. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.3411-10.2010 · Cited by 277
Glymphatic influx and clearance are accelerated by neurovascular coupling
Holstein-Rønsbo, S., Gan, Y., Giannetto, M. J., Rasmussen, M. K., Sigurdsson, B., Beinlich, F. R. M., Rose, L., Untiet, V., Hablitz, L. M., Kelley, D. H., et al. (2023) · Nature Neuroscience · doi:10.1038/s41593-023-01327-2 · Cited by 248
Analysis of the brain mural cell transcriptome
He, L., Vanlandewijck, M., Raschperger, E., Andaloussi Mäe, M., Jung, B., Lebouvier, T., Ando, K., Hofmann, J., Keller, A., & Betsholtz, C. (2016) · Scientific Reports · doi:10.1038/srep35108 · Cited by 233
Act1 mediates IL-17-induced EAE pathogenesis selectively in NG2+ glial cells
Kang, Z., Wang, C., Zepp, J., Wu, L., Sun, K., Zhao, J., Chandrasekharan, U., DiCorleto, P. E., Trapp, B. D., Ransohoff, R. M., et al. (2013) · Nature Neuroscience · doi:10.1038/nn.3505 · Cited by 218
Nemes-Baran, A. D., White, D. R., & DeSilva, T. M. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108047 · Cited by 196
Clarke, L. E., Young, K. M., Hamilton, N. B., Li, H., Richardson, W. D., & Attwell, D. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.0928-12.2012 · Cited by 182
Antidepressant Effects of Fibroblast Growth Factor-2 in Behavioral and Cellular Models of Depression
Elsayed, M., Banasr, M., Duric, V., Fournier, N. M., Licznerski, P., & Duman, R. S. (2012) · Biological Psychiatry · doi:10.1016/j.biopsych.2012.03.003 · Cited by 169
PDGFRα signaling drives adipose tissue fibrosis by targeting progenitor cell plasticity
Iwayama, T., Steele, C., Yao, L., Dozmorov, M. G., Karamichos, D., Wren, J. D., & Olson, L. E. (2015) · Genes & Development · doi:10.1101/gad.260554.115 · Cited by 164
The translational biology of remyelination: past, present, and future
Franklin, R. J. M., & Gallo, V. (2014) · Glia · doi:10.1002/glia.22622 · Cited by 162
De Biase, L. M., Kang, S. H., Baxi, E. G., Fukaya, M., Pucak, M. L., Mishina, M., Calabresi, P. A., & Bergles, D. E. (2011) · The Journal of Neuroscience · doi:10.1523/jneurosci.2455-11.2011 · Cited by 161
Self-citationControl of oligodendroglial cell number by the miR-17-92 cluster
Budde, H., Schmitt, S., Fitzner, D., Opitz, L., Salinas-Riester, G., & Simons, M. (2010) · Development · doi:10.1242/dev.050633 · Cited by 152
Notch3 Is Necessary for Blood Vessel Integrity in the Central Nervous System
Henshall, T. L., Keller, A., He, L., Johansson, B. R., Wallgard, E., Raschperger, E., Mäe, M. A., Jin, S., Betsholtz, C., & Lendahl, U. (2015) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.114.304849 · Cited by 145
Differential cell proliferation in the cortex of the appsweps1de9 alzheimer's disease mouse model
Kamphuis, W., Orre, M., Kooijman, L., Dahmen, M., & Hol, E. M. (2012) · Glia · doi:10.1002/glia.22295 · Cited by 137
Špiranec, K., Chen, W., Werner, F., Nikolaev, V. O., Naruke, T., Koch, F., Werner, A., Eder-Negrin, P., Diéguez-Hurtado, R., Adams, R. H., et al. (2018) · Circulation · doi:10.1161/circulationaha.117.033383 · Cited by 135
Oligodendrocyte progenitor cells in Alzheimer's disease: from physiology to pathology
Zou, P., Wu, C., Liu, T. C.-Y., Duan, R., & Yang, L. (2023) · Translational Neurodegeneration · doi:10.1186/s40035-023-00385-7 · Cited by 97
Baxi, E. G., DeBruin, J., Jin, J., Strasburger, H. J., Smith, M. D., Orthmann‐Murphy, J. L., Schott, J. T., Fairchild, A. N., Bergles, D. E., & Calabresi, P. A. (2017) · Glia · doi:10.1002/glia.23229 · Cited by 97
Self-citationOligodendrocyte Lineage Cells Contribute Unique Features to Rett Syndrome Neuropathology
Nguyen, M. V. C., Felice, C. A., Du, F., Covey, M. V., Robinson, J. K., Mandel, G., & Ballas, N. (2013) · The Journal of Neuroscience · doi:10.1523/jneurosci.2657-13.2013 · Cited by 91
Properties of human astrocytes and NG2 glia
Bedner, P., Jabs, R., & Steinhäuser, C. (2020) · Glia · doi:10.1002/glia.23725 · Cited by 71
Bribián, A., Fontana, X., Llorens, F., Gavín, R., Reina, M., García-Verdugo, J. M., Torres, J. M., de Castro, F., & Río, J. A. D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033872 · Cited by 70
Local production of astrocytes in the cerebral cortex
Ge, W.-P., & Jia, J.-M. (2016) · Neuroscience · doi:10.1016/j.neuroscience.2015.08.057 · Cited by 58
“…al. (1993) Retroviral labeling Rat Levison and Goldman (1993) Retroviral labeling Mouse Marshall and Goldman (2002) NG2 glia Genetic tracing Mouse Zhu et al. (2008) Genetic tracing Mouse Guo et al. (2009) Locally proliferating glia Radioautography Rat Kaplan and Hinds (1980) Retroviral labeling…”
BackgroundResultInfluentialImproved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules
Hsu, C.-W., Poché, R. A., Saik, J. E., Ali, S., Wang, S., Yosef, N., Calderon, G. A., Scott, L., Vadakkan, T. J., Larina, I. V., et al. (2015) · PLOS ONE · doi:10.1371/journal.pone.0131643 · Cited by 56
Korte, N., James, G., You, H., Hirunpattarasilp, C., Christie, I., Sethi, H., & Attwell, D. (2023) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x231152549 · Cited by 49
Zimmer, T. S., Broekaart, D. W. M., Gruber, V.-E., van Vliet, E. A., Mühlebner, A., & Aronica, E. (2020) · Frontiers in Neurology · doi:10.3389/fneur.2020.01028 · Cited by 48
Rodriguez, E. G., Wegner, C., Kreutzfeldt, M., Neid, K., Thal, D. R., Jürgens, T., Brück, W., Stadelmann, C., & Merkler, D. (2014) · Acta Neuropathologica · doi:10.1007/s00401-014-1260-8 · Cited by 44
Retinoic acid-loaded NFL-lipid nanocapsules promote oligodendrogenesis in focal white matter lesion
Carradori, D., Labrak, Y., Miron, V. E., Saulnier, P., Eyer, J., Préat, V., & des Rieux, A. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2019.119653 · Cited by 38
Auderset, L., Pitman, K. A., Cullen, C. L., Pepper, R. E., Taylor, B. V., Foa, L., & Young, K. M. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.564351 · Cited by 30
Progenies of NG2 glia: what do we learn from transgenic mouse models ?
Huang, W., Guo, Q., & Scheller, A. (2021) · Neural Regeneration Research · doi:10.4103/1673-5374.286950 · Cited by 20
Bonnar, O., Shaw, K., Anderle, S., Grijseels, D. M., Clarke, D., Bell, L., King, S. L., & Hall, C. N. (2023) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x231172842 · Cited by 8
Metadata
- Authors
- Zhu, X., Bergles, D. E., & Nishiyama, A.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2008-01-01
- Volume
- 135
- Issue
- 1
- Pages
- 145-157
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2103279601
- Cited by
- 683
- References
- 7
- FWCI
- 8.09
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
