Cited by 4,331Meta-analysis and systematic reviewsStatistical Methods in EpidemiologyAcademic Writing and Publishing

The STROBE guidelines

Open access
Cuschieri, Sarah·2019·Saudi Journal of Anaesthesia

Abstract

An observational study is a type of epidemiological study design, which can take the form of a cohort, a case-control, or a cross-sectional study. When presenting observational studies in manuscripts, an author needs to ascertain a clear presentation of the work and provide the reader with appropriate information to enable critical appraisal of the research. The Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines were created to aid the author in ensuring high-quality presentation of the conducted observational study. The original articles publishing the STROBE guidelines together with their bibliographies were identified and thoroughly reviewed. These guidelines consist of 22 checklist items that the author needs to fulfil before submitting the manuscript to a journal. The STROBE guidelines were created to aid the authors in presenting their work and not to act as a validation tool for the conducted study or as a framework to conduct an observational study on. The authors complying with these guidelines are more likely to succeed in publishing their observational study work in a journal.

AI snapshot

Written from the paper’s full text.

TL;DR

This article is a narrative overview of the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines, intended to help authors present cohort, case–control, and cross-sectional studies clearly enough for critical appraisal. It walks through the 22-item checklist and explains what each section of a manuscript should report.

Key findings

  • The STROBE guidelines consist of 22 checklist items; 18 are common to all three observational designs (cohort, case–control, cross-sectional), while 4 items (numbers 6, 12, 14, and 15) vary by study design.
  • The guidelines were developed in 2004 through a collaborative initiative of epidemiologists, methodologists, statisticians, researchers, and journal editors.
  • The author stresses that STROBE is not a methodological framework for conducting observational studies, nor a quality-evaluation instrument, nor a means of standardizing manuscripts — it is an aid to transparent reporting.
  • The guidelines discourage the terms "prospective," "retrospective," "concurrent," and "historical," instead encouraging authors to describe the actual methodology.

Methods

  • Design: narrative review/educational overview of the STROBE guidelines.
  • Data: the original articles publishing the STROBE guidelines and their bibliographies were identified and thoroughly reviewed; the checklist is reproduced from Vandenbroucke et al.
  • Analysis: descriptive, item-by-item explanation of the 22 checklist items grouped by manuscript section (title/abstract, introduction, methods, results, discussion, other information).

Limitations

  • Not stated in the paper: the article reports no formal limitations section and declares no conflicts of interest and no financial support.
  • Evident from the text: the review describes no systematic search strategy, inclusion criteria, or appraisal of the source literature, so it functions as an explanatory summary rather than a systematic review.
  • Evident from the text: the article presents no empirical data or evaluation of whether compliance with STROBE actually improves reporting quality or publication outcomes, despite asserting that complying authors "are more likely to succeed in publishing."

Model-written summary. Check it against the paper before relying on it.

Integrity signals

Registry

Crossref · 10.4103/sja.sja_543_18

Cites

7 of 7 references verified0 citation errors · 0 not checkable

Statistics

Nothing to recompute.

Claims the paper makes

0 of 12 cited sources checked · 0 supported · 0 flagged

  • Premisecitation

    Adequate reporting of observational study information is of vital importance because it determines whether the established results can be included in systematic reviews later on.

    “Such study information is of vital importance in a manuscript since this will determine whether the established results can be included in systemic reviews later on.[34]”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites [34]

      Not checked in the free run.

  • Classified as probably not a substantial claim. 10
  • May combine two claims. 4
  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation

    The STROBE guidelines were developed in 2004 through a collaborative initiative of epidemiologists, methodologists, statisticians, researchers, and journal editors.

    “To ascertain high-quality reporting of observational studies, the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines were developed following a collaborative initiative of epidemiologists, methodologists, statisticians, researchers, and journal editors in 2004.[1]”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation

    The STROBE guidelines enable the journal's editor, reviewers, and readers to critically appraise the study.

    “Furthermore, the STROBE guidelines enable the journal's editor, reviewers, and the readers to critically appraise the study.[1]”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation

    Eighteen of the 22 STROBE checklist items are common to all three observational designs: cohort, cross-sectional, and case-control studies.

    “Eighteen items are common to all the three observational designs, that is, cohort, cross-sectional, and case–control studies.”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S2 (checkable).

    Interpretationreasoning

    Having a thorough understanding of the STROBE guidelines is becoming a requisite for authors who wish to conduct and publish an observational study.

    “Having a thorough understanding of the STROBE guidelines is therefore becoming a requisite for authors who wish to conduct and publish an observational study.”
    Quote in the paperNot a substantial claim
    • Rests on an assumption: A substantial number of journals are requesting authors to follow the STROBE guidelines before submitting their observational-study-inspired manuscript.
  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation

    The STROBE guidelines consist of 22 checklist items.

    “These guidelines consist of 22 checklist items that the author needs to fulfil before submitting the manuscript to a journal.”
    • Also stated in: § The Strobe Checklist · plain
      “A total of 22 checklist items contribute to the STROBE guidelines.”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • Classified as probably not a substantial claim. May combine two claims.

    Premisecitation

    The STROBE guidelines were created to aid authors in ensuring clear, high-quality presentation and reporting of observational studies, including what was planned, done, found, and concluded, and to enable assessment of the study's strengths and weaknesses.

    “The Strengthening the Reporting of Observational studies in Epidemiology (STROBE) guidelines were created to aid the author in ensuring high-quality presentation of the conducted observational study.”
    • Also stated in: § Introduction · plain
      “These guidelines were created to aid in the presentation of the conducted observational study to ensure adequate reporting (what was planned, done, found, and concluded) as well as assessment of the strengths and weaknesses of the study.[12]”
    • Also stated in: § The STROBE Guidelines · plain
      “The aim of the STROBE guidelines was to provide a readily available checklist to ensure a clear presentation of what was planned and conducted in an observational study.”
    Quote in the paperSubstantial claim0 of 1 sources checked
    • cites [12]

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.

    Premisecitation

    The STROBE guidelines were not aimed to bring forward standardization of manuscripts but rather to encourage the production of interesting and narrative articles while maintaining transparency.

    “Furthermore, the guidelines were not aimed to bring forward standardization of manuscripts but rather to encourage the production of interesting and narrative articles while maintaining transparency.[5]”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [5] Schriger, 2005, Ann Emerg Med

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S3 (load-bearing).

    Premisecitation

    The remaining four STROBE checklist items (items 6, 12, 14, and 15) have specific variations according to the study design.

    “However, the remaining four checklist items (items number 6, 12, 14, and 15) have specific variations according to the study design.”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • May combine two claims.

    Premisecitation

    The STROBE guidelines were not created to act as a validation tool for the conducted study, an instrument for quality evaluation of observational research, or a methodological framework for conducting an observational study.

    “The STROBE guidelines were created to aid the authors in presenting their work and not to act as a validation tool for the conducted study or as a framework to conduct an observational study on.”
    • Also stated in: § The STROBE Guidelines · plain
      “In no way were these guidelines established to provide a methodological framework for conducting an observational study.[1]”
    • Also stated in: § The STROBE Guidelines · plain
      “Nor were the guidelines developed as an instrument for quality evaluation of observational research.[2]”
    Quote in the paperSubstantial claim0 of 2 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

    • cites [2] von, 2007, BMJ

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.

    Premisecitation

    The STROBE guidelines do not allow the use of the words 'prospective', 'retrospective', 'concurrent', or 'historical', but rather encourage the researcher to describe the actual methodology.

    “The STROBE guidelines do not allow the use of the words “prospective” or “retrospective” or “concurrent” or “historical,” but rather encourage the researcher to describe the actual methodology.[1]”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

  • Probably not a substantial claim: may not pass S2 (checkable), S3 (load-bearing).

    Premisecitation

    Reporting the method used to establish the study size along with the confidence intervals considered is essential for the reader to ascertain whether sufficient statistical precision has been attained.

    “The method used to establish the study size needs to be reported along with the confidence intervals considered. This is essential for the reader to ascertain whether sufficient statistical precision has been attained in the study.[1]”
    Quote in the paperNot a substantial claim0 of 1 sources checked
    • cites [1] Vandenbroucke, 2007, PLoS Med

      Not checked in the free run.

11 of 11
  • The paper relies on this without support.

    A substantial number of journals are requesting authors to follow the STROBE guidelines before submitting their observational-study-inspired manuscript.

    “A substantial number of journals are requesting authors to follow the STROBE guidelines before submitting their observational-study-inspired manuscript.”
  • The paper relies on this without support.

    Authors complying with the STROBE guidelines are more likely to succeed in publishing their observational study work in a journal.

    “The authors complying with these guidelines are more likely to succeed in publishing their observational study work in a journal.”
  • The paper relies on this without support.

    Causality of a particular outcome cannot be established in most study designs, unless a longitudinal cohort study has been conducted.

    “Furthermore, researchers have to keep in mind that causality of a particular outcome cannot be established in most study designs, unless a longitudinal cohort study has been conducted.”
3 of 3

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Cites

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Authors
Cuschieri, S.
Affiliations
University of Malta (MT)
Corresponding author
Cuschieri, Sarah
Venue
Saudi Journal of Anaesthesia
Published
2019
Volume
13
Issue
5
Pages
31
ISSN
  • 0975-3125 (ISSN-L)
  • 1658-354X
OpenAlex
W2915365125
Cited by
4,331
References
8
Citations by year
2026: 1091 · 2025: 1137 · 2024: 944 · 2023: 580 · 2022: 335 · 2021: 181 · 2020: 57 · 2019: 4
FWCI
96.42
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Saudi Journal of Anaesthesia (publishedVersion, other-oa)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
  • OAR@UM (University of Malta) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Topics
  • Social Sciences › Decision Sciences › Statistics, Probability and Uncertainty › Meta-analysis and systematic reviews
  • Physical Sciences › Mathematics › Statistics and Probability › Statistical Methods in Epidemiology
  • Social Sciences › Arts and Humanities › History and Philosophy of Science › Academic Writing and Publishing
Keywords
STROBE guidelines, reporting quality, observational studies, manuscript preparation, critical appraisal, reporting checklist, scientific publications

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Classified by jev-1.13-20260917 (gold-guideline-v0.2) on 2026-10-09.

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