Cited by 45Exercise and Physiological ResponsesMuscle Physiology and DisordersCardiovascular and exercise physiology

Interpretation of exercise-induced changes in human skeletal muscle mRNA expression depends on the timing of the post-exercise biopsies

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Kuang, Jujiao, McGinley, Cian, Lee, Matthew J-C, Saner, Nicholas J., Garnham, Andrew, Bishop, David J.·2022·PeerJ

Published version of a preprint

Abstract

Background: Exercise elicits a range of adaptive responses in skeletal muscle, which include changes in mRNA expression. To better understand the health benefits of exercise training, it is important to investigate the underlying molecular mechanisms of skeletal muscle adaptation to exercise. However, most studies have assessed the molecular events at only a few time-points within a short time frame post-exercise, and the variations of gene expression kinetics have not been addressed systematically. Methods: We assessed the mRNA expression of 23 gene isoforms implicated in the adaptive response to exercise at six time-points (0, 3, 9, 24, 48, and 72 h post exercise) over a 3-day period following a single session of high-intensity interval exercise. Results: The temporal patterns of target gene expression were highly variable and the expression of mRNA transcripts detected was largely dependent on the timing of muscle sampling. The largest fold change in mRNA expression of each tested target gene was observed between 3 and 72 h post-exercise. Discussion and Conclusions: Our findings highlight an important gap in knowledge regarding the molecular response to exercise, where the use of limited time-points within a short period post-exercise has led to an incomplete understanding of the molecular response to exercise. Muscle sampling timing for individual studies needs to be carefully chosen based on existing literature and preliminary analysis of the molecular targets of interest. We propose that a comprehensive time-course analysis on the exercise-induced transcriptional response in humans will significantly benefit the field of exercise molecular biology.

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Authors
Kuang, J., McGinley, C., Lee, M. J.-C., Saner, N. J., Garnham, A., & Bishop, D. J.
Affiliations
  • Australian Institute for Musculoskeletal Science (AU)
  • Sportscotland Institute of Sport, Stirling, United Kingdom
  • Victoria University (AU)
  • Baker Heart and Diabetes Institute (AU)
Corresponding author
Bishop, David J.
Venue
PeerJ
Publisher
PeerJ
Published
2022-02-04
Volume
10
Pages
e12856
ISSN
2167-8359 (ISSN-L)
OpenAlex
W4225998726
Cited by
45
References
92
Citations by year
2026: 5 · 2025: 10 · 2024: 10 · 2023: 8 · 2022: 7 · 2021: 3 · 2020: 2
FWCI
3.78
Type
journal article
Language
en
Open access
Locations
  • PeerJ (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Victoria University Research Repository (Victoria University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Own your potential (DEAKIN) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
Australian Research Council (DP140104165)
Topics
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
exercise-induced gene expression, skeletal muscle, mRNA expression kinetics, high-intensity interval exercise, exercise adaptation, gene isoforms, time-course analysis
MeSH terms
Biopsy, Humans, Kinetics, RNA, Messenger, Exercise, Muscle, Skeletal

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