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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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“The assay was conducted according to Bradford [15].”
From Functional and Bioactive Characterization of Hemp Cake Proteins and Polyphenols from Non-Psychoactive Cannabis sativa (2025) · cites this paper as Bradford [15]
In the citing paper
“The Bradford method for protein determination (Bradford, 1976) is based on the colour change in Coomassie blue G250 when it reacts with proteins.”
From Chemical methods for the characterization of proteolysis in cheese during ripening (1997) · cites this paper as Bradford, 1976
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“The resulting supernatant was removed and the pelleted microsomes were then re-suspended in 100mM Tris-HCl, pH 9.0 and protein concentration was determined by the method of Bradford (1976) using BSA as a standard.”
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Metadata
- Authors
- Bradford, M. M.
- Affiliations
- University of Georgia (US)
- Corresponding author
- Bradford, Marion M.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 1976-05
- Volume
- 72
- Issue
- 1-2
- Pages
- 248-254
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W2128635872
- Cited by
- 255,302
- References
- 20
- Citations by year
- 2026: 4653 · 2025: 6079 · 2024: 6445 · 2023: 6652 · 2022: 7284 · 2021: 7677 · 2020: 7719 · 2019: 7373 · 2018: 7128 · 2017: 6899 · 2016: 7250 · 2015: 7450 · 2014: 7796 · 2013: 7638 · 2012: 7600
- FWCI
- 124.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- protein quantification, Coomassie Brilliant Blue G-250, dye-protein binding, colorimetric assay, absorption maximum shift, detergent interference, sodium dodecyl sulfate, Triton X-100
- MeSH terms
- Binding Sites, Colorimetry, Methods, Microchemistry, Protein Binding, Proteins, Rosaniline Dyes, Time Factors
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