No differences in muscle protein synthesis rates following ingestion of wheat protein, milk protein, and their protein blend in healthy, young males
Open accessAbstract
Plant-derived proteins have been suggested to have less anabolic properties when compared with animal-derived proteins. Whether blends of plant- and animal-derived proteins can compensate for their lesser anabolic potential has not been assessed. The present study compares post-prandial muscle protein synthesis rates following the ingestion of milk protein with wheat protein or a blend of wheat plus milk protein in healthy, young males. In a randomised, double-blind, parallel-group design, 36 males (23 (sd 3) years) received a primed continuous L-[ring-13C6]-phenylalanine infusion after which they ingested 30 g milk protein (MILK), 30 g wheat protein (WHEAT) or a 30 g blend combining 15 g wheat plus 15 g milk protein (WHEAT+MILK). Blood and muscle biopsies were collected frequently for 5 h to assess post-prandial plasma amino acid profiles and subsequent myofibrillar protein synthesis rates. Ingestion of protein increased myofibrillar protein synthesis rates in all treatments (P < 0·001). Post-prandial myofibrillar protein synthesis rates did not differ between MILK v. WHEAT (0·053 (sd 0·013) v. 0·056 (sd 0·012) %·h-1, respectively; t test P = 0·56) or between MILK v. WHEAT+MILK (0·053 (sd 0·013) v. 0·059 (sd 0·025) %·h-1, respectively; t test P = 0·46). In conclusion, ingestion of 30 g milk protein, 30 g wheat protein or a blend of 15 g wheat plus 15 g milk protein increases muscle protein synthesis rates in young males. Furthermore, muscle protein synthesis rates following the ingestion of 30 g milk protein do not differ from rates observed after ingesting 30 g wheat protein or a blend with 15 g milk plus 15 g wheat protein in healthy, young males.
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No differences in postprandial MPS rates were observed between isonitrogenous 30 g servings of milk and wheat protein in young men.
“and no differences in postprandial MPS rates were observed between isonitrogenous (30 g) servings of milk and wheat protein in young men [89].”
From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [89]
A plant/animal protein blend or exclusively plant protein blend can stimulate an equivalent MPS response to an animal-based comparison.
“A handful of studies have supported the blending theory by demonstrating that a plant/animal protein blend [89,90,92,93] or exclusively plant protein blend [88,94,95] can stimulate an equivalent MPS response to an animal-based comparison.”
From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [89]
There is a growing number of studies that contradict, or at least obfuscate, the narrative that nonanimal proteins are inferior.
“However, there is a growing number of studies that contradict, or at least obfuscate, this narrative [53,[88], [89], [90], [91]].”
From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [89]
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Slow and fast dietary proteins differently modulate postprandial protein accretion
Boirie, Y., Dangin, M., Gachon, P., Vasson, M.-P., Maubois, J.-L., & Beaufrère, B. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.26.14930 · Cited by 1,453 · see citing works
Converting Nitrogen into Protein—Beyond 6.25 and Jones' Factors
Mariotti, F., Tomé, D., & Mirand, P. P. (2008) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408390701279749 · Cited by 1,409 · see citing works
Protein content and amino acid composition of commercially available plant-based protein isolates
Gorissen, S. H. M., Crombag, J. J. R., Senden, J. M. G., Waterval, W. A. H., Bierau, J., Verdijk, L. B., & van Loon, L. J. C. (2018) · Amino Acids · doi:10.1007/s00726-018-2640-5 · Cited by 1,169 · see citing works
Self-citationPercutaneous Needle Biopsy of Skeletal Muscle in Physiological and Clinical Research
Bergström, J. P. (1975) · Scandinavian Journal of Clinical and Laboratory Investigation · doi:10.3109/00365517509095787 · Cited by 1,159 · see citing works
Sustainability of meat-based and plant-based diets and the environment
Pimentel, D., & Pimentel, M. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.3.660s · Cited by 1,021 · see citing works
Tang, J. E., Moore, D. R., Kujbida, G. W., Tarnopolsky, M. A., & Phillips, S. M. (2009) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00076.2009 · Cited by 998 · see citing works
Volpi, E., Kobayashi, H., Sheffield-Moore, M., Mittendorfer, B., & Wolfe, R. R. (2003) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/78.2.250 · Cited by 846 · see citing works
Moore, D. R., Churchward-Venne, T. A., Witard, O., Breen, L., Burd, N. A., Tipton, K. D., & Phillips, S. M. (2015) · The Journals of Gerontology: Series A · doi:10.1093/gerona/glu103 · Cited by 770 · see citing works
Plant proteins in relation to human protein and amino acid nutrition
Young, V., & Pellett, P. (1994) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/59.5.1203s · Cited by 717 · see citing works
Pennings, B., Boirie, Y., Senden, J. M., Gijsen, A. P., Kuipers, H., & van Loon, L. J. (2011) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.008102 · Cited by 656 · see citing works
Self-citationThe Skeletal Muscle Anabolic Response to Plant- versus Animal-Based Protein Consumption
van Vliet, S., Burd, N. A., & van Loon, L. J. (2015) · The Journal of Nutrition · doi:10.3945/jn.114.204305 · Cited by 651 · see citing works
Self-citationBreen, L., & Phillips, S. M. (2011) · Nutrition & Metabolism · doi:10.1186/1743-7075-8-68 · Cited by 576 · see citing works
Wilkinson, S. B., Tarnopolsky, M. A., MacDonald, M. J., MacDonald, J. R., Armstrong, D., & Phillips, S. M. (2007) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/85.4.1031 · Cited by 530 · see citing works
Wilkinson, D. J., Hossain, T., Hill, D. S., Phillips, B. E., Crossland, H., Williams, J., Loughna, P., Churchward‐Venne, T. A., Breen, L., Phillips, S. M., et al. (2013) · The Journal of Physiology · doi:10.1113/jphysiol.2013.253203 · Cited by 484 · see citing works
Rieu, I., Balage, M., Sornet, C., Giraudet, C., Pujos, E., Grizard, J., Mosoni, L., & Dardevet, D. (2006) · The Journal of Physiology · doi:10.1113/jphysiol.2006.110742 · Cited by 410 · see citing works
Anabolic Resistance of Muscle Protein Synthesis with Aging
Burd, N. A., Gorissen, S. H., & van Loon, L. J. C. (2013) · Exercise and Sport Sciences Reviews · doi:10.1097/jes.0b013e318292f3d5 · Cited by 384 · see citing works
Self-citationThe effects of plant-based diets on the body and the brain: a systematic review
Medawar, E., Hühn, S., Villringer, A., & Witte, A. V. (2019) · Translational Psychiatry · doi:10.1038/s41398-019-0552-0 · Cited by 355 · see citing works
Aging is accompanied by a blunted muscle protein synthetic response to protein ingestion
Wall, B. T., Gorissen, S. H., Pennings, B., Koopman, R., Groen, B. B. L., Verdijk, L. B., & van Loon, L. J. C. (2015) · PLOS ONE · doi:10.1371/journal.pone.0140903 · Cited by 352 · see citing works
Self-citationKoopman, R., Wagenmakers, A. J. M., MANDERS, R. J. F., Zorenc, A. H. G., Senden, J. M., Gorselink, M., KEIZER, H. A., & van Loon, L. J. C. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00413.2004 · Cited by 316 · see citing works
Tieland, M., Borgonjen-Van den Berg, K. J., van Loon, L. J. C., & de Groot, L. C. P. G. M. (2012) · European Journal of Nutrition · doi:10.1007/s00394-011-0203-6 · Cited by 297 · see citing works
Yang, Y., Churchward-Venne, T. A., Burd, N. A., Breen, L., Tarnopolsky, M. A., & Phillips, S. M. (2012) · Nutrition & Metabolism · doi:10.1186/1743-7075-9-57 · Cited by 286 · see citing works
Burd, N. A., Yang, Y., Moore, D. R., Tang, J. E., Tarnopolsky, M. A., & Phillips, S. M. (2012) · British Journal of Nutrition · doi:10.1017/s0007114511006271 · Cited by 276 · see citing works
Tanner, R. E., Brunker, L. B., Agergaard, J., Barrows, K. M., Briggs, R. A., Kwon, O. S., Young, L. M., Hopkins, P. N., Volpi, E., Marcus, R. L., et al. (2015) · The Journal of Physiology · doi:10.1113/jp270699 · Cited by 223 · see citing works
Gorissen, S. H., Horstman, A. M., Franssen, R., Crombag, J. J., Langer, H., Bierau, J., Respondek, F., & van Loon, L. J. (2016) · The Journal of Nutrition · doi:10.3945/jn.116.231340 · Cited by 221 · see citing works
Gorissen, S. H. M., & Witard, O. C. (2018) · Proceedings of the Nutrition Society · doi:10.1017/s002966511700194x · Cited by 221 · see citing works
Leucine co-ingestion improves post-prandial muscle protein accretion in elderly men
Wall, B. T., Hamer, H. M., de Lange, A., Kiskini, A., Groen, B. B. L., Senden, J. M. G., Gijsen, A. P., Verdijk, L. B., & van Loon, L. J. C. (2013) · Clinical Nutrition · doi:10.1016/j.clnu.2012.09.002 · Cited by 220 · see citing works
Self-citationMaximizing muscle protein anabolism: the role of protein quality
Tang, J. E., & Phillips, S. M. (2009) · Current Opinion in Clinical Nutrition & Metabolic Care · doi:10.1097/mco.0b013e32831cef75 · Cited by 178 · see citing works
Protein blend ingestion following resistance exercise promotes human muscle protein synthesis
Reidy, P. T., Walker, D. K., Dickinson, J. M., Gundermann, D. M., Drummond, M. J., Timmerman, K. L., Fry, C. S., Borack, M. S., Cope, M. B., Mukherjea, R., et al. (2013) · The Journal of Nutrition · doi:10.3945/jn.112.168021 · Cited by 178 · see citing works
Koopman, R., Walrand, S., Beelen, M., Gijsen, A. P., Kies, A. K., Boirie, Y., Saris, W. H. M., & van Loon, L. J. C. (2009) · The Journal of Nutrition · doi:10.3945/jn.109.109173 · Cited by 167 · see citing works
Post-Prandial Protein Handling: You Are What You Just Ate
Groen, B. B. L., Horstman, A. M. H., Hamer, H. M., de Haan, M. W., van Kranenburg, J. M. X., Bierau, J., Poeze, M., Wodzig, W. K. W. H., Rasmussen, B. B., & van Loon, L. J. C. (2015) · PLoS ONE · doi:10.1371/journal.pone.0141582 · Cited by 150 · see citing works
Protein Considerations for Optimising Skeletal Muscle Mass in Healthy Young and Older Adults
Witard, O., Wardle, S., Macnaughton, L., Hodgson, A., & Tipton, K. (2016) · Nutrients · doi:10.3390/nu8040181 · Cited by 132 · see citing works
Nutritional Interventions to Promote Post-Exercise Muscle Protein Synthesis
Koopman, R., Saris, W. H. M., Wagenmakers, A. J. M., & van Loon, L. J. C. (2007) · Sports Medicine · doi:10.2165/00007256-200737100-00005 · Cited by 111 · see citing works
The muscle protein synthetic response to food ingestion
Gorissen, S. H. M., Rémond, D., & van Loon, L. J. C. (2015) · Meat Science · doi:10.1016/j.meatsci.2015.05.009 · Cited by 90 · see citing works
Borack, M. S., Reidy, P. T., Husaini, S. H., Markofski, M. M., Deer, R. R., Richison, A. B., Lambert, B. S., Cope, M. B., Mukherjea, R., Jennings, K., et al. (2016) · The Journal of Nutrition · doi:10.3945/jn.116.231159 · Cited by 75 · see citing works
Churchward-Venne, T. A., Pinckaers, P. J. M., Smeets, J. S. J., Peeters, W. M., Zorenc, A. H., Schierbeek, H., Rollo, I., Verdijk, L. B., & van Loon, L. J. C. (2019) · The Journal of Nutrition · doi:10.1093/jn/nxy251 · Cited by 51 · see citing works
Self-citationBurd, N. A., Pennings, B., Groen, B. B. L., Gijsen, A. P., Senden, J. M., & van Loon, L. J. C. (2012) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00513.2012 · Cited by 38 · see citing works
Churchward-Venne, T. A., Pinckaers, P. J., Smeets, J. S., Peeters, W. M., Zorenc, A. H., Schierbeek, H., Rollo, I., Verdijk, L. B., & van Loon, L. J. (2019) · The Journal of Nutrition · doi:10.1093/jn/nxy244 · Cited by 31 · cited 1× · see citing works
“To minimise dilution of the steady-state plasma L-[ring-(13)C6]phenylalanine precursor pool, the phenylalanine content of each protein drink was enriched with 3·85 % free, crystalline L-[ring-(13)C6]-phenylalanine((21,27)).”
Weijzen, M. E. G., Kouw, I. W. K., Verschuren, A. A. J., Muyters, R., Geurts, J. A. P., Emans, P. J., Geerlings, P., Verdijk, L. B., & van Loon, L. J. C. (2019) · The journal of nutrition health & aging · doi:10.1007/s12603-019-1157-2 · Cited by 27 · see citing works
Self-citationMass Spectrometry and Stable Isotopes in Nutritional and Pediatric Research
(2017) · Wiley eBooks · doi:10.1002/9781119341185 · Cited by 20 · see citing works
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- Authors
- Pinckaers, P. J. M., Kouw, I. W. K., Hendriks, F. K., van Kranenburg, J. M. X., de Groot, L. C. P. G. M., Verdijk, L. B., Snijders, T., & van Loon, L. J. C.
- Affiliations
- Maastricht University Medical Centre (NL)
- Venue
- British Journal Of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 2021-02-18
- Volume
- 126
- Issue
- 12
- Pages
- 1832-1842
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W3133100105
- Cited by
- 76
- References
- 39
- Citations by year
- 2026: 13 · 2025: 13 · 2024: 18 · 2023: 11 · 2022: 16 · 2021: 5
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, cc-by)
- Socio-Environmental Systems Modeling (acceptedVersion, cc-by)
- VUBIR (Vrije Universiteit Brussel) (submittedVersion)
- Research Publications (Maastricht University) (submittedVersion, cc-by)
- Data Archiving and Networked Services (DANS) (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- muscle protein synthesis, wheat proteins, milk protein, protein blends, plant proteins, animal protein, myofibrillar protein synthesis, young men
- MeSH terms
- Dietary Proteins, Double-Blind Method, Eating, Humans, Male, Milk Proteins, Muscle Proteins, Triticum, Muscle, Skeletal, Postprandial Period, Young Adult
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