In Vivo Real-Time Pharmaceutical Evaluations of Near-Infrared II Fluorescent Nanomedicine Bound Polyethylene Glycol Ligands for Tumor Photothermal Ablation
Abstract
Pharmaceutical evaluations of nanomedicines are of great significance for their further launch into industry and clinic. Near-infrared (NIR) fluorescence imaging plays essential roles in preclinical drug development by providing important insights into the biodistributions of drugs in vivo with deep tissue penetration and high spatiotemporal resolution. However, NIR-II fluorescence imaging has rarely been exploited for in vivo real-time pharmaceutical evaluations of nanomedicine. Herein, we developed a highly emissive NIR-II luminophore to establish a versatile nanoplatform to noninvasively monitor the in vivo metabolism of nanomedicines bound various polyethylene glycol (PEG) ligands in a real-time manner. An alternative D-A-D conjugated oligomer (DTTB) was synthesized to achieve NIR-II emission peaked at ∼1050 nm with high fluorescence QYs of 13.4% and a large absorption coefficient. By anchoring with the DTTB molecule, intrinsically fluorescent micelles were fabricated and bound with PEG ligands at various chain lengths. In vivo NIR-II fluorescence and photoacoustic imaging results revealed that an appropriate PEG chain length could effectively contribute to the longer blood circulation and better tumor targeting. In vivo therapeutic experiments also confirmed the optimized nanomedicines have efficient photothermal elimination of tumors and good biosafety. This work offered an alternative highly fluorescent NIR-II material and demonstrated a promising approach for real-tim…
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Each methylene unit inserted between donor and acceptor typically induces a red-shift of ~100 nm.
“A general rule, as exemplified in cyanine dyes, is that each methylene unit inserted between donor and acceptor typically induces a red-shift of ~100 nm111–123.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [116]
The BBTD core and its derivatives are potent electron-accepting units with excellent quantum efficiency, making them ideal for low-bandgap polymers and long-wavelength NIR-II fluorophores.
“The benzobisthiadiazole (BBTD) core and its derivatives are potent electron-accepting units with excellent quantum efficiency, making them ideal for the design of low-bandgap polymers and long-wavelength NIR-II fluorophores (Fig. 5 and Table 2)114–150.”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [116]
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- Authors
- Li, S., Chen, H., Liu, H., Liu, L., Yuan, Y., Mao, C., Zhang, W., Zhang, X., Guo, W., Lee, C.-S., & Liang, X.-J.
- Affiliations
- City University of Hong Kong (HK)
- Tianjin University (CN)
- Chinese Academy of Sciences (CN)
- Second Affiliated Hospital of Guangzhou Medical University (CN)
- Corresponding author
- Guo, Weisheng
- Lee, Chun-Sing
- Liang, Xing-Jie
- ORCID
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-09-14
- Volume
- 14
- Issue
- 10
- Pages
- 13681-13690
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W3086018990
- Cited by
- 60
- References
- 53
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 8 · 2023: 15 · 2022: 6 · 2021: 15 · 2020: 1
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Research Grants Council, University Grants Committee (JLFS/P-101/18)
- Deutsche Forschungsgemeinschaft (31761133013)
- National Research Foundation Singapore (R279-000-444-281, R279-000-483-281)
- City University of Hong Kong (9667179)
- National University of Singapore (R279-000-482-133)
- National Natural Science Foundation of China (31430031, 31630027, 31761133013, 31971302, 81601603)
- Chinese Academy of Sciences (XDA09030301)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence Properties of Advanced Materials
- Keywords
- NIR-II fluorescence imaging, nanomedicine, PEG chain length, photothermal ablation, tumor targeting, blood circulation, photoacoustic imaging
- MeSH terms
- Theranostic Nanomedicine, Humans, Hyperthermia, Induced, Ligands, Neoplasms, Phototherapy, Polyethylene Glycols, Nanomedicine, Photoacoustic Techniques
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