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Addressing preferred specimen orientation in single-particle cryo-EM through tilting
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We present a strategy for tackling preferred specimen orientation in single-particle cryogenic electron microscopy by employing tilts during data collection. We also describe a tool to quantify the resulting directional resolution using 3D Fourier shell correlation volumes. We applied these methods to determine the structures at near-atomic resolution of the influenza hemagglutinin trimer, which adopts a highly preferred specimen orientation, and of ribosomal biogenesis intermediates, which adopt moderately preferred orientations.
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Metadata
- Authors
- Tan, Y. Z., Baldwin, P. R., Davis, J. H., Williamson, J. R., Potter, C. S., Carragher, B., & Lyumkis, D.
- Venue
- Nature Methods
- Published
- 2017-07-03
- Volume
- 14
- Issue
- 8
- Pages
- 793-796
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2728385286
- Cited by
- 903
- References
- 2
- FWCI
- 23.56
- Type
- journal article
- Language
- en
- Open access
- green
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