The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Insulin selectively increases SREBP-1c mRNA in the livers of rats with streptozotocin-induced diabetes
Open accessAbstract
Sterol regulatory element binding proteins (SREBPs) enhance transcription of genes encoding enzymes of cholesterol and fatty acid biosynthesis and uptake. In the current experiments, we observed a decline in the mRNA encoding one SREBP isoform, SREBP-1c, in the livers of rats that were rendered diabetic by treatment with streptozotocin. There was no change in the mRNA encoding SREBP-1a, which is derived from the same gene as SREBP-1c but uses a different promoter. The ratio of SREBP-1c:1a transcripts fell 25-fold from 5:1 in control rats to 0.2:1 in the diabetic animals. The SREBP-1c mRNA rose nearly to normal, and the 1c:1a ratio increased 17-fold when the diabetic rats were treated for 6 h with insulin. These treatments produced no change in the mRNA for SREBP-2, which is encoded by a separate gene. The SREBP-1c mRNA also fell selectively in freshly isolated rat hepatocytes and rose when the cells were treated with insulin. Considered together with recent data on hepatocytes [Foretz, M., Pacot, C., Dugal, I., et al. (1999) Mol. Cell. Biol. 19, 3760–3768], the current in vivo studies suggest that insulin may stimulate lipid synthesis in the liver by selectively inducing transcription of the SREBP-1c gene.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Protein–protein interaction in insulin signaling and the molecular mechanisms of insulin resistance
Virkamäki, A., Ueki, K., & Kahn, C. R. (1999) · Journal of Clinical Investigation · doi:10.1172/jci6609 · Cited by 854
What if Minkowski Had Been Ageusic? An Alternative Angle on Diabetes
McGarry, J. D. (1992) · Science · doi:10.1126/science.1439783 · Cited by 657
Cited by
Insulin signalling and the regulation of glucose and lipid metabolism
Saltiel, A. R., & Kahn, C. R. (2001) · Nature · doi:10.1038/414799a · Cited by 5,326
SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver
Horton, J. D., Goldstein, J. L., & Brown, M. S. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15593 · Cited by 4,794
Self-citationSREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver
Horton, J. D., Goldstein, J. L., & Brown, M. S. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215593 · Cited by 4,092
Self-citationObesity and the regulation of energy balance
Spiegelman, B. M., & Flier, J. S. (2001) · Cell · doi:10.1016/s0092-8674(01)00240-9 · Cited by 2,398
Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and clinical implications
Fabbrini, E., Sullivan, S., & Klein, S. (2010) · Hepatology · doi:10.1002/hep.23280 · Cited by 2,100
Molecular mediators of hepatic steatosis and liver injury
Browning, J. D., & Horton, J. D. (2004) · Journal of Clinical Investigation · doi:10.1172/jci200422422 · Cited by 1,772
Self-citationProtein Sensors for Membrane Sterols
Goldstein, J. L., DeBose-Boyd, R. A., & Brown, M. S. (2006) · Cell · doi:10.1016/j.cell.2005.12.022 · Cited by 1,517
Self-citationMcGarry, J. D. (2002) · Diabetes · doi:10.2337/diabetes.51.1.7 · Cited by 1,435
Targeting bile‐acid signalling for metabolic diseases
Thomas, C., Pellicciari, R., Pruzanski, M., Auwerx, J., & Schoonjans, K. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2619 · Cited by 1,422
A Century of Cholesterol and Coronaries: From Plaques to Genes to Statins
Goldstein, J. L., & Brown, M. S. (2015) · Cell · doi:10.1016/j.cell.2015.01.036 · Cited by 1,233
Self-citationMichael, M. D., Kulkarni, R. N., Postic, C., Previs, S. F., Shulman, G. I., Magnuson, M. A., & Kahn, C. R. (2000) · Molecular Cell · doi:10.1016/s1097-2765(05)00015-8 · Cited by 1,296
SREBP-regulated lipid metabolism: convergent physiology - divergent pathophysiology
Shimano, H., & Sato, R. (2017) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2017.91 · Cited by 1,174
Insulin resistance drives hepatic de novo lipogenesis in nonalcoholic fatty liver disease
Smith, G. I., Shankaran, M., Yoshino, M., Schweitzer, G. G., Chondronikola, M., Beals, J. W., Okunade, A. L., Patterson, B. W., Nyangau, E., Field, T., et al. (2020) · Journal of Clinical Investigation · doi:10.1172/jci134165 · Cited by 771
Li, S., Brown, M. S., & Goldstein, J. L. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914798107 · Cited by 733
Self-citationKammoun, H. L., Chabanon, H., Hainault, I., Luquet, S., Magnan, C., Koike, T., Ferré, P., & Foufelle, F. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37007 · Cited by 732
Role of Dietary Fructose and Hepatic De Novo Lipogenesis in Fatty Liver Disease
Softic, S., Cohen, D. E., & Kahn, C. R. (2016) · Digestive Diseases and Sciences · doi:10.1007/s10620-016-4054-0 · Cited by 709
Liang, G., Yang, J., Horton, J. D., Hammer, R. E., Goldstein, J. L., & Brown, M. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m111421200 · Cited by 669
Self-citationOsborne, T. F. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.r000017200 · Cited by 521
Dissociation of Hepatic Steatosis and Insulin Resistance in Mice Overexpressing DGAT in the Liver
Monetti, M., Levin, M. C., Watt, M. J., Sajan, M. P., Marmor, S., Hubbard, B. K., Stevens, R. D., Bain, J. R., Newgard, C. B., Farese, R. V., et al. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.05.005 · Cited by 511
Mechanisms by which Dietary Fatty Acids Modulate Plasma Lipids
Fernandez, M. L., & West, K. L. (2005) · The Journal of Nutrition · doi:10.1093/jn/135.9.2075 · Cited by 469
Biddinger, S. B., Hernandez-Ono, A., Rask-Madsen, C., Haas, J. T., Alemán, J. O., Suzuki, R., Scapa, E. F., Agarwal, C., Carey, M. C., Stephanopoulos, G., et al. (2008) · Cell Metabolism · doi:10.1016/j.cmet.2007.11.013 · Cited by 469
Insulin signaling in health and disease
Saltiel, A. R. (2021) · Journal of Clinical Investigation · doi:10.1172/jci142241 · Cited by 446
Kim, C.-W., Addy, C., Kusunoki, J., Anderson, N. N., Deja, S., Fu, X., Burgess, S. C., Li, C., Ruddy, M., Chakravarthy, M., et al. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.07.009 · Cited by 419
Self-citationMoon, Y.-A., Liang, G., Xie, X., Frank-Kamenetsky, M., Fitzgerald, K., Koteliansky, V., Brown, M. S., Goldstein, J. L., & Horton, J. D. (2012) · Cell Metabolism · doi:10.1016/j.cmet.2011.12.017 · Cited by 320
Self-citationDiabetes and apoptosis: lipotoxicity
Kusminski, C. M., Shetty, S., Orci, L., Unger, R. H., & Scherer, P. E. (2009) · Apoptosis · doi:10.1007/s10495-009-0352-8 · Cited by 302
Matsuda, M., Korn, B. S., Hammer, R. E., Moon, Y.-A., Komuro, R., Horton, J. D., Goldstein, J. L., Brown, M. S., & Shimomura, I. (2001) · Genes & Development · doi:10.1101/gad.891301 · Cited by 303
Self-citationBo, T., Gao, L., Yao, Z., Shao, S., Wang, X., Proud, C. G., & Zhao, J. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.04.006 · Cited by 293
New Perspectives on Atherogenesis: Role of Abnormal Triglyceride-Rich Lipoprotein Metabolism
Ginsberg, H. N. (2002) · Circulation · doi:10.1161/01.cir.0000035280.64322.31 · Cited by 255
Osborne, T. F., & Espenshade, P. J. (2009) · Genes & Development · doi:10.1101/gad.1854309 · Cited by 238
Regional differences of insulin action in adipose tissue: insights from in vivo and in vitro studies
Giorgino, F., Laviola, L., & Eriksson, J. W. (2005) · Acta Physiologica Scandinavica · doi:10.1111/j.1365-201x.2004.01385.x · Cited by 230
Promoter Analysis of the Mouse Sterol Regulatory Element-binding Protein-1c Gene
Amemiya-Kudo, M., Shimano, H., Yoshikawa, T., Yahagi, N., Hasty, A. H., Okazaki, H., Tamura, Y., Shionoiri, F., Iizuka, Y., Ohashi, K., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m005353200 · Cited by 226
Decreased lipid synthesis in livers of mice with disrupted Site-1 protease gene
Yang, J., Goldstein, J. L., Hammer, R. E., Moon, Y.-A., Brown, M. S., & Horton, J. D. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.201524598 · Cited by 217
Self-citationDeBose-Boyd, R. A., Ou, J., Goldstein, J. L., & Brown, M. S. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.4.1477 · Cited by 209
Self-citationCoactivator-Dependent Acetylation Stabilizes Members of the SREBP Family of Transcription Factors
Giandomenico, V., Simonsson, M., Grünroos, E., & Ericsson, J. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.7.2587-2599.2003 · Cited by 205
Longevity, lipotoxicity and leptin: the adipocyte defense against feasting and famine
Unger, R. H. (2005) · Biochimie · doi:10.1016/j.biochi.2004.11.014 · Cited by 184
PPARα is necessary for the lipopenic action of hyperleptinemia on white adipose and liver tissue
Lee, Y., Yu, X., Gonzales, F., Mangelsdorf, D. J., Wang, M.-Y., Richardson, C., Witters, L. A., & Unger, R. H. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.182420899 · Cited by 181
Nagashima, K., Lopez, C., Donovan, D., Ngai, C., Fontanez, N., Bensadoun, A., Fruchart-Najib, J., Holleran, S., Cohn, J. S., Ramakrishnan, R., et al. (2005) · Journal of Clinical Investigation · doi:10.1172/jci200523219 · Cited by 201
Botolin, D., Wang, Y., Christian, B., & Jump, D. B. (2006) · Journal of Lipid Research · doi:10.1194/jlr.m500365-jlr200 · Cited by 162
MicroRNA 33 Regulates Glucose Metabolism
Ramírez, C. M., Goedeke, L., Rotllan, N., Yoon, J.-H., Cirera-Salinas, D., Mattison, J. A., Suárez, Y., de Cabo, R., Gorospe, M., & Fernández-Hernando, C. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.00016-13 · Cited by 154
Gene expression profile of rat adipose tissue at the onset of high-fat-diet obesity
Li, J., Yu, X., Pan, W., & Unger, R. H. (2002) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00516.2001 · Cited by 153
Zhang, Y.-L., Hernandez-Ono, A., Siri, P., Weisberg, S., Conlon, D., Graham, M. J., Crooke, R. M., Huang, L.-S., & Ginsberg, H. N. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m604709200 · Cited by 147
Rong, S., Cortés, V. A., Rashid, S., Anderson, N. N., McDonald, J. G., Liang, G., Moon, Y.-A., Hammer, R. E., & Horton, J. D. (2017) · eLife · doi:10.7554/elife.25015 · Cited by 134
Self-citationInteractions between hepatic iron and lipid metabolism with possible relevance to steatohepatitis
Ahmed, U. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i34.4651 · Cited by 121
van der Meer, R. W., Hammer, S., Lamb, H. J., Frölich, M., Diamant, M., Rijzewijk, L. J., de Roos, A., Romijn, J. A., & Smit, J. W. A. (2008) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2007-2524 · Cited by 113
SREBP-1c Mediates the Insulin-dependent Hepatic Glucokinase Expression
Kim, S.-Y., Kim, H.-I., Kim, T.-H., Im, S.-S., Park, S.-K., Lee, I.-K., Kim, K.-S., & Ahn, Y.-H. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m313223200 · Cited by 108
Rong, S., Xia, M., Vale, G., Wang, S., Kim, C.-W., Li, S., McDonald, J. G., Radhakrishnan, A., & Horton, J. D. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.01.011 · Cited by 102
Self-citationGut microbiota modulation in Alzheimer's disease: Focus on lipid metabolism
Bonfili, L., Cuccioloni, M., Gong, C., Cecarini, V., Spina, M., Zheng, Y., Angeletti, M., & Eleuteri, A. M. (2022) · Clinical Nutrition · doi:10.1016/j.clnu.2022.01.025 · Cited by 69
Sajan, M. P., Standaert, M. L., Rivas, J., Miura, A., Kanoh, Y., Soto, J., Taniguchi, C. M., Kahn, C. R., & Farese, R. V. (2009) · Diabetologia · doi:10.1007/s00125-009-1336-5 · Cited by 60
Disturbed lipid metabolism in glycogen storage disease type 1
Bandsma, R., Smit, P., & Kuipers, F. (2002) · European Journal of Pediatrics · doi:10.1007/s00431-002-1007-8 · Cited by 81
Hepatic deletion of Mboat7 (LPIAT1) causes activation of SREBP-1c and fatty liver
Xia, M., Chandrasekaran, P., Rong, S., Fu, X., & Mitsche, M. A. (2021) · Journal of Lipid Research · doi:10.1194/jlr.ra120000856 · Cited by 60
Warrier, M., Hinds, T. D., Ledford, K. J., Cash, H. A., Patel, P. R., Bowman, T. A., Stechschulte, L. A., Yong, W., Shou, W., Najjar, S. M., et al. (2010) · Endocrinology · doi:10.1210/en.2009-1158 · Cited by 57
Regulation of fatty acid and cholesterol synthesis: co-operation or competition?
Gibbons, G. F. (2003) · Progress in Lipid Research · doi:10.1016/s0163-7827(03)00034-1 · Cited by 51
Early growth response 1 (Egr1) regulates cholesterol biosynthetic gene expression
Gokey, N. G., Lopez-Anido, C., Gillian-Daniel, A. L., & Svaren, J. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.263509 · Cited by 51
Insulin Regulation of Hepatic Lipid Homeostasis
Uehara, K., Santoleri, D., Whitlock, A. E. G., & Titchenell, P. M. (2023) · Comprehensive Physiology · doi:10.1002/cphy.c220015 · Cited by 49
Metabolic injury of hepatocytes promotes progression of NAFLD and AALD
Carvalho-Gontijo, R., Han, C., Zhang, L., Zhang, V., Hosseini, M., Mekeel, K., Schnabl, B., Loomba, R., Karin, M., Brenner, D. A., et al. (2022) · Seminars in Liver Disease · doi:10.1055/s-0042-1755316 · Cited by 23
Ren, Y., Ren, Q., Lu, J., Guo, X., Huo, X., Ji, L., & Yang, X. (2018) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.2960 · Cited by 18
Zhang, Z.-H., Liu, X.-Q., Zhang, C., He, W., Wang, H., Chen, Y.-H., Liu, X.-J., Chen, X., & Xu, D.-X. (2016) · Scientific Reports · doi:10.1038/srep33513 · Cited by 18
Physiological and pathophysiological actions of insulin in the liver
Kubota, N., Kubota, T., & Kadowaki, T. (2025) · Endocrine Journal · doi:10.1507/endocrj.ej24-0192 · Cited by 19
Attie, A. D. (2004) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000137288.82390.04 · Cited by 16
Metadata
- Authors
- Shimomura, I., Bashmakov, Y., Ikemoto, S., Horton, J. D., Brown, M. S., & Goldstein, J. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1999-11-23
- Volume
- 96
- Issue
- 24
- Pages
- 13656-13661
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2030610610
- Cited by
- 793
- References
- 2
- FWCI
- 13.74
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
