Clonal populations of T cells in normal elderly humans: the T cell equivalent to "benign monoclonal gammapathy".

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Posnett, D N, Sinha, R, Kabak, S, Russo, C·1994·The Journal of experimental medicine

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Abstract

To determine whether T cells, like B cells, can become clonally expanded in normal individuals as a function of age, we compared the T cell V beta repertoire of cord blood to that of peripheral blood from normal donors over 65 yr of age. T cells from elderly subjects contained expanded subsets (greater than the mean+three standard deviations) of T cell receptor (TCR) V beta populations. These expanded subsets were observed primarily among CD8, but not CD4 cells, represented up to 37.5% of all CD8 cells, and were present in most elderly subjects. An expanded V beta 5.2/3 CD8 subset and a V beta 6.7a CD8 subset from separate donors were analyzed by reverse transcriptase-polymerase chain reaction, cloning and sequencing of the TCR beta chain VDJ junction. In both cases the expanded subsets were mono- or oligoclonal while control CD4 populations were polyclonal. Using two-color flow cytometry it was possible to identify the expanded V beta 6.7a subset as CD8+ CD28-CD11b+ cells. In three of five random old subjects similar expansions of V beta subsets were found specifically in the CD8+ CD28- subpopulation, an interesting subset of cytotoxic T lymphocytes, known to lack proliferative responses to TCR stimuli. It is common practice to use the demonstration of clonality as a diagnostic indicator for T cell lymphoma/leukemia. In view of the high frequency of expanded T clones of T cells in normal elderly subjects the diagnostic usefulness of this test should be reexamined.

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Authors: Posnett, D. N., Sinha, R., Kabak, S., & Russo, C.
Venue: The Journal of experimental medicine
Publisher: Rockefeller University Press
Published: 1994-02-01
Volume: 179
Issue: 2
Pages: 609-618
ISSN: 0022-1007 (ISSN-L)
1540-9538
DOI: 10.1084/jem.179.2.609
OpenAlex: W2133908456
Cited by: 760
References: 0
FWCI: 15.92
Type: journal article
Language: en
Open access: green

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Clonal populations of T cells in normal elderly humans: the T cell equivalent to "benign monoclonal gammapathy".

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