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Cited by 666Body Composition Measurement TechniquesMuscle metabolism and nutritionChemical Reactions and Isotopes

Total body water measurement in humans with 18O and 2H labeled water

Schoeller, D A, van Santen, E, Peterson, D W, Dietz, W, Jaspan, J, Klein, P D·1980·The American Journal of Clinical Nutrition
DOI 10.1093/ajcn/33.12.2686
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Abstract

Deuterium or tritium labeled water traditionally has been used for the measurement of total body water by application of the dilution principle. However, these methods have not enjoyed wide clinical use. The use of deuterium is hampered by the tedious and time consuming nature of the analysis while the use of tritium involves a radiation hazard. In addition, exchange of the label with nonaqueous hydrogen in the body raises questions about the accuracy of total body water values. To determine if water labeled with the stable isotope 18O can yield faster and more accurate results, total body water was measured simultaneously using water labeled with both 18O and 2H. The 18O and 3H dilutions were measured by mass spectrometry. The relative precision of the body water value using the 18O method was 2% for both serum and breath analysis. The 18O was fully equilibrated within 2 to 3 hr after administration, and results from the analysis of breath CO2 could be readily obtained within 1 hr after sampling. The H2(18)O dilution space averaged 3.0% (SE = 0.4) less than the 2HHO dilution space, because the latter exchanges with nonaqueous hydrogen. For this reason, the H2(18)O dilution should be a more accurate measure of total body water than the 3HHO dilution.

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Crossref · 10.1093/ajcn/33.12.2686

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  • The reference method measured total body water by stable-isotope dilution with deuterium and 18O; fat-free mass was then derived from total body water with a hydration factor of 0.73.

    “Total body water was measured as a constituent of the measurement of total energy expenditure by doubly labeled water, using dilution with stable isotopes, i.e., the average of total body water spaces as measured by deuterium and 18oxygen was used as the reference method (21). Fat-free mass was calculated from total body water using a hydration constant (0.73) (22).”

    From Prediction of fat-free mass using bioelectrical impedance analysis in young adults from five populations of African origin (2013) · cites this paper as [21]

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Authors
Schoeller, D. A., van Santen, E., Peterson, D. W., Dietz, W., Jaspan, J., & Klein, P. D.
Affiliations
  • Hewlett-Packard (United States) (US)
  • Massachusetts Institute of Technology (US)
ORCID
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
1980-12
Volume
33
Issue
12
Pages
2686-2693
ISSN
0002-9165 (ISSN-L)
DOI
10.1093/ajcn/33.12.2686
OpenAlex
W1808407909
Cited by
666
References
30
Citations by year
2026: 6 · 2025: 9 · 2024: 5 · 2023: 4 · 2022: 15 · 2021: 9 · 2020: 14 · 2019: 11 · 2018: 13 · 2017: 13 · 2016: 6 · 2015: 20 · 2014: 9 · 2013: 12 · 2012: 15
FWCI
3.23
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • American Journal of Clinical Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Body Composition Measurement Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Chemical Reactions and Isotopes
Keywords
total body water, dilution method, stable isotope tracers, serum dilution, mass spectrometry
MeSH terms
Body Water, Breath Tests, Carbon Dioxide, Deuterium, Female, Humans, Male, Obesity, Oxygen Isotopes, Mass Spectrometry

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