Enzymatic Determination of Total Serum Cholesterol

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Allain, Charles C, Poon, Lucy S, Chan, Cicely S G, Richmond, W, Fu, Paul C·1974·Clinical Chemistry

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Abstract

An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximum absorption at 500 nm. The method is reproducible, and the results correlate well with those obtained by automated Liebermann—Burchard procedures (AA-2 and SMA 12/60) and the method of Abell et al. The present method affords better specificity than those previously reported and has excellent precision.

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Authors: Allain, C. C., Poon, L. S., Chan, C. S. G., Richmond, W., & Fu, P. C.
Venue: Clinical Chemistry
Publisher: Oxford University Press (OUP)
Published: 1974-04-01
Volume: 20
Issue: 4
Pages: 470-475
ISSN: 0009-9147 (ISSN-L)
1530-8561
DOI: 10.1093/clinchem/20.4.470
OpenAlex: W1946263363
Cited by: 9,125
References: 0
FWCI: 27.79
Type: journal article
Language: en
Open access: bronze

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Enzymatic Determination of Total Serum Cholesterol

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