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Cited by 9,125

The DOI matches its Crossref record.

Enzymatic Determination of Total Serum Cholesterol

Open access
Allain, Charles C, Poon, Lucy S, Chan, Cicely S G, Richmond, W, Fu, Paul C·1974·Clinical Chemistry
DOI 10.1093/clinchem/20.4.470
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Abstract

An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-one with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipyrine and phenol in the presence of peroxidase to yield a chromogen with maximum absorption at 500 nm. The method is reproducible, and the results correlate well with those obtained by automated Liebermann—Burchard procedures (AA-2 and SMA 12/60) and the method of Abell et al. The present method affords better specificity than those previously reported and has excellent precision.

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Metadata

Authors
Allain, C. C., Poon, L. S., Chan, C. S. G., Richmond, W., & Fu, P. C.
Venue
Clinical Chemistry
Publisher
Oxford University Press (OUP)
Published
1974-04-01
Volume
20
Issue
4
Pages
470-475
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
DOI
10.1093/clinchem/20.4.470
OpenAlex
W1946263363
Cited by
9,125
References
0
FWCI
27.79
Type
journal article
Language
en
Open access
bronze

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.