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Extracellular vesicle isolation and characterization: toward clinical application
Open accessAbstract
Two broad categories of extracellular vesicles (EVs), exosomes and shed microvesicles (sMVs), which differ in size distribution as well as protein and RNA profiles, have been described. EVs are known to play key roles in cell-cell communication, acting proximally as well as systemically. This Review discusses the nature of EV subtypes, strategies for isolating EVs from both cell-culture media and body fluids, and procedures for quantifying EVs. We also discuss proteins selectively enriched in exosomes and sMVs that have the potential for use as markers to discriminate between EV subtypes, as well as various applications of EVs in clinical diagnosis.
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Cites
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., et al. (2015) · Nature · doi:10.1038/nature14581 · Cited by 2,880
Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver
Costa-Silva, B., Aiello, N. M., Ocean, A. J., Singh, S., Zhang, H., Thakur, B. K., Becker, A., Hoshino, A., Mark, M. T., Molina, H., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3169 · Cited by 2,692
Regulation of immune responses by extracellular vesicles
Robbins, P. D., & Morelli, A. E. (2014) · Nature Reviews Immunology · doi:10.1038/nri3622 · Cited by 2,260
Filipe, V., Hawe, A., & Jiskoot, W. (2010) · Pharmaceutical Research · doi:10.1007/s11095-010-0073-2 · Cited by 1,885
Christianson, H. C., Svensson, K. J., van Kuppevelt, T. H., Li, J.-P., & Belting, M. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1304266110 · Cited by 903
Webber, J., & Clayton, A. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.19861 · Cited by 817
Isolation and characterization of platelet-derived extracellular vesicles
Aatonen, M. T., Öhman, T., Nyman, T. A., Laitinen, S., Grönholm, M., & Siljander, P. R. ‐. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24692 · Cited by 333
Elevated concentration of microvesicles isolated from peripheral blood in breast cancer patients
Galindo-Hernandez, O., Villegas-Comonfort, S., Candanedo, F., González-Vázquez, M.-C., Chavez-Ocaña, S., Jimenez-Villanueva, X., Sierra-Martinez, M., & Salazar, E. P. (2013) · Archives of Medical Research · doi:10.1016/j.arcmed.2013.03.002 · Cited by 152
Cited by
Concise review: MSC-derived exosomes for cell-free therapy
Phinney, D. G., & Pittenger, M. F. (2017) · Stem Cells · doi:10.1002/stem.2575 · Cited by 1,834
Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory
Poggio, M., Hu, T., Pai, C.-C., Chu, B., Belair, C. D., Chang, A., Montabana, E., Lang, U. E., Fu, Q., Fong, L., et al. (2019) · Cell · doi:10.1016/j.cell.2019.02.016 · Cited by 1,376
Exosomes: key players in cancer and potential therapeutic strategy
Dai, J., Su, Y., Zhong, S., Cong, L., Liu, B., Yang, J., Tao, Y., He, Z., Chen, C., & Jiang, Y. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00261-0 · Cited by 1,186
Application of exosomes as liquid biopsy in clinical diagnosis
Zhou, B., Xu, K., Zheng, X., Chen, T., Wang, J., Song, Y., Shao, Y., & Zheng, S. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00258-9 · Cited by 685
Efficient RNA drug delivery using red blood cell extracellular vesicles
Usman, W. M., Pham, T. C., Kwok, Y. Y., Vu, L. T., Ma, V., Peng, B., Chan, Y. S., Wei, L., Chin, S. M., Azad, A., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04791-8 · Cited by 663
Extracellular vesicles: novel promising delivery systems for therapy of brain diseases.
Rufino-Ramos, D., Albuquerque, P. R., Carmona, V., Perfeito, R., Nobre, R. J., & Pereira de Almeida, L. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.07.001 · Cited by 418
Regulation of exosome production and cargo sorting
Wei, H., Chen, Q., Lin, L., Sha, C., Li, T., Liu, Y., Yin, X., Xu, Y., Chen, L., Gao, W., et al. (2021) · International Journal of Biological Sciences · doi:10.7150/ijbs.53671 · Cited by 385
The function and clinical application of extracellular vesicles in innate immune regulation
Zhou, X., Xie, F., Wang, L., Zhang, L., Zhang, S., Fang, M., & Zhou, F. (2020) · Cellular & Molecular Immunology · doi:10.1038/s41423-020-0391-1 · Cited by 294
New windows into the brain: Central nervous system-derived extracellular vesicles in blood
Shi, M., Sheng, L., Stewart, T., Zabetian, C. P., & Zhang, J. (2019) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2019.01.005 · Cited by 181
McKeever, P. M., Schneider, R., Taghdiri, F., Weichert, A., Multani, N., Brown, R. A., Boxer, A. L., Karydas, A., Miller, B., Robertson, J., et al. (2018) · Molecular Neurobiology · doi:10.1007/s12035-018-1032-x · Cited by 166
Chen, J., & Chopp, M. (2018) · Stroke · doi:10.1161/strokeaha.117.018292 · Cited by 152
Extracellular vesicles: mediators and biomarkers of pathology along CNS barriers
Ramirez, S. H., Andrews, A. M., Paul, D., & Pachter, J. S. (2018) · Fluids and Barriers of the CNS · doi:10.1186/s12987-018-0104-7 · Cited by 128
Zhong, L., Wang, J., Wang, P., Liu, X., Liu, P., Cheng, X., Cao, L., Wu, H., Chen, J., & Zhou, L. (2023) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03409-1 · Cited by 110
Strategies for the use of Extracellular Vesicles for the Delivery of Therapeutics
Sil, S., Dagur, R. S., Liao, K., Peeples, E. S., Hu, G., Periyasamy, P., & Buch, S. (2020) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-019-09873-y · Cited by 109
Exosomal PD‐L1: New Insights Into Tumor Immune Escape Mechanisms and Therapeutic Strategies
Zhou, K., Guo, S., Li, F., Sun, Q., & Liang, G. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.569219 · Cited by 99
Astrocyte-derived extracellular vesicles: A double-edged sword in central nervous system disorders
Zhao, S., Sheng, S., Wang, Y., Ding, L., Xu, X., Xia, X., & Zheng, J. C. (2021) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2021.02.027 · Cited by 90
Extracellular vesicles: translational challenges and opportunities
Clemmens, H., & Lambert, D. W. (2018) · Biochemical Society Transactions · doi:10.1042/bst20180112 · Cited by 70
A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides
Zhang, N., Sun, N., & Deng, C. (2020) · Chemical Communications · doi:10.1039/d0cc06147f · Cited by 69
Matrix Bound Nanovesicles Have Tissue-Specific Characteristics That Suggest a Regulatory Role
Turner, N. J., Quijano, L. M., Hussey, G. S., Jiang, P., & Badylak, S. F. (2022) · Tissue Engineering Part A · doi:10.1089/ten.tea.2022.0091 · Cited by 32
Jiang, S., Li, X., Li, Y., Chang, Z., Yuan, M., Zhang, Y., Zhu, H., Xiu, Y., Cong, H., Yin, L., et al. (2024) · Science Translational Medicine · doi:10.1126/scitranslmed.adg5116 · Cited by 30
Self-citationLiu, Y., Xia, Y., Smollar, J., Mao, W., & Wan, Y. (2021) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2021.188539 · Cited by 23
Minic, Z., Li, Y., Hüttmann, N., Uppal, G. K., D’Mello, R., & Berezovski, M. V. (2023) · Biomedicines · doi:10.3390/biomedicines11041076 · Cited by 20
Clinical implications of extracellular vesicles in neurodegenerative diseases
Vinaiphat, A., & Sze, S. K. (2019) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2019.1657407 · Cited by 20
Extracellular vesicle therapy for obesity-induced NAFLD: a comprehensive review of current evidence
Zhang, J., Ma, B., Wang, Z., Chen, Y., Li, C., & Dong, Y. (2024) · Cell Communication and Signaling · doi:10.1186/s12964-023-01292-0 · Cited by 17
Research progress of exosomes in the angiogenesis of digestive system tumour
Liu, Y., Wu, H., Sang, Y., Chong, W., Shang, L., & Li, L. (2024) · Discover Oncology · doi:10.1007/s12672-024-00879-4 · Cited by 7
Metadata
- Authors
- Xu, R., Greening, D. W., Zhu, H.-J., Takahashi, N., & Simpson, R. J.
- Venue
- Journal of Clinical Investigation
- Published
- 2016-04-01
- Volume
- 126
- Issue
- 4
- Pages
- 1152-1162
- ISSN
- 0021-9738 (ISSN-L)
- 1558-8238
- OpenAlex
- W2325305253
- Cited by
- 882
- References
- 10
- FWCI
- 35.96
- Type
- journal article
- Language
- en
- Open access
- bronze
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