Optimizing neuronal differentiation from induced pluripotent stem cells to model ASD
Open accessAbstract
Autism spectrum disorder (ASD) is an early-onset neurodevelopmental disorder characterized by deficits in social communication, and restricted and repetitive patterns of behavior. Despite its high prevalence, discovery of pathophysiological mechanisms underlying ASD has lagged due to a lack of appropriate model systems. Recent advances in induced pluripotent stem cell (iPSC) technology and neural differentiation techniques allow for detailed functional analyses of neurons generated from living individuals with ASD. Refinement of cortical neuron differentiation methods from iPSCs will enable mechanistic studies of specific neuronal subpopulations that may be preferentially impaired in ASD. In this review, we summarize recent accomplishments in differentiation of cortical neurons from human pluripotent stems cells and efforts to establish in vitro model systems to study ASD using personalized neurons.
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Differentiation of induced pluripotent stem cells into neural cells recapitulates many neurodevelopmental features observed in prenatal human tissue and occurs in a temporal sequence that roughly corresponds to in vivo corticogenesis.
“Although these models come with their own limitations, including variability and limited functional maturation (Box 1), the differentiation of iPSCs into neural cells recapitulates many of the neurodevelopmental features observed in prenatal human tissue and occurs in a temporal sequence that roughly corresponds to in vivo corticogenesis29–31.”
From Consequences of trisomy 21 for brain development in Down syndrome (2024) · cites this paper as [31]
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The Evolution of Stem Cells, Disease Modeling, and Drug Discovery for Neurological Disorders
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- Authors
- Kim, D.-S., Ross, P. J., Zaslavsky, K., & Ellis, J.
- Affiliations
- Hospital for Sick Children (CA)
- University of Toronto (CA)
- Corresponding author
- Ellis, James
- Venue
- Frontiers in Cellular Neuroscience
- Publisher
- Frontiers Media SA
- Published
- 2014-04-11
- Volume
- 8
- Pages
- 109-109
- ISSN
- 1662-5102 (ISSN-L)
- PMC
- PMC3990101
- OpenAlex
- W2008468014
- Cited by
- 70
- References
- 139
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 5 · 2022: 1 · 2021: 3 · 2020: 9 · 2019: 7 · 2018: 5 · 2017: 4 · 2016: 12 · 2015: 19 · 2014: 2
- FWCI
- 5.06
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Cellular Neuroscience (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- IslandScholar (University of Prince Edward Island) (submittedVersion, cc-by)
- Funders
- Ontario Brain Institute
- National Research Foundation
- National Research Foundation of Korea (2012039296)
- Ministry of Education, Science and Technology
- Canadian Institute for Military and Veteran Health Research
- National Institutes of Health
- Canadian Institutes of Health Research (129129_1)
- National Institute of Mental Health (R33MH087908)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- autism spectrum disorder, induced pluripotent stem cells, cortical neuron differentiation, in vitro disease model, neurodevelopmental disorders, neuronal subpopulations
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