CoChat
Sign Up
Cited by 246Gut microbiota and healthGastrointestinal motility and disordersAnimal Nutrition and Physiology

Memory and learning behavior in mice is temporally associated with diet-induced alterations in gut bacteria

Li, Wang, Dowd, Scot E., Scurlock, Bobbie, Acosta-Martinez, Veronica, Lyte, Mark·2009·Physiology & Behavior
DOI 10.1016/j.physbeh.2008.12.004
Open this paper in CoChat — free account

Abstract

The ability of dietary manipulation to influence learning and behavior is well recognized and almost exclusively interpreted as direct effects of dietary constituents on the central nervous system. The role of dietary modification on gut bacterial populations and the possibility of such microbial population shifts related to learning and behavior is poorly understood. The purpose of this study was to examine whether shifts in bacterial diversity due to dietary manipulation could be correlated with changes in memory and learning. Five week old male CF1 mice were randomly assigned to receive standard rodent chow (PP diet) or chow containing 50% lean ground beef (BD diet) for 3 months. As a measure of memory and learning, both groups were trained and tested on a hole-board open field apparatus. Following behavioral testing, all mice were sacrificed and colonic stool samples collected and analyzed by automated rRNA intergenic spacer analysis (ARISA) and bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) approach for microbial diversity. Results demonstrated significantly higher bacterial diversity in the beef supplemented diet group according to ARISA and bTEFAP. Compared to the PP diet, the BD diet fed mice displayed improved working (P=0.0008) and reference memory (P<0.0001). The BD diet fed animals also displayed slower speed (P<0.0001) in seeking food as well as reduced anxiety level in the first day of testing (P=0.0004). In conclusion, we observed a correlation between dietary induced shifts in bacteria diversity and animal behavior that may indicate a role for gut bacterial diversity in memory and learning.

Integrity signals

Registry

Crossref · 10.1016/j.physbeh.2008.12.004

Cites

81 of 81 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The L. casei intervention mitigates intestinal barrier dysfunction, inhibits microglia activation, and protects brain neurons from oxidative stress and neuroinflammation.

    “The intervention was shown to mitigate intestinal barrier dysfunction, inhibit the activation of microglia, and protect brain neurons from oxidative stress and neuroinflammation.”

    From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [502]

  • In a D-galactose/aluminum chloride-induced Alzheimer's disease model, selenium nanoparticles-enriched L. casei (ATCC 393) administered for thirteen weeks significantly improves cognitive dysfunction and minimizes β-amyloid aggregation, Tau hyperphosphorylation, and neuronal death by modulating the Akt/CREB/BDNF signaling pathway.

    “In a D-galactose/aluminum chloride-induced model of Alzheimer’s disease, selenium nanoparticles-enriched L. casei (ATCC 393) administered for thirteen weeks significantly improved cognitive dysfunction and minimized β-amyloid aggregation, hyperphosphorylation of Tau protein, and prevented neuronal death by modulating the Akt/cAMP-response element binding protein/BDNF signaling pathway (502).”

    From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [502]

  • Proposed mechanisms linking poor diet and obesity to cognitive dysfunction include changes in GM composition and function, alterations in microbial metabolites, compromised barrier integrity, peripheral and central inflammatory processes, and hormonal, glucoregulatory, and cardiovascular changes.

    “Proposed mechanisms include changes in GM composition and function, alterations in microbial metabolites, compromised barrier integrity, and peripheral and central inflammatory processes, as well as hormonal, glucoregulatory, and cardiovascular changes (502, 511, 512).”

    From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [502]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Li, W., Dowd, S. E., Scurlock, B., Acosta-Martinez, V., & Lyte, M.
Affiliations
  • Texas Tech University (US)
  • Cropping Systems Research Laboratory (US)
Corresponding author
Lyte, Mark
ORCID
Venue
Physiology & Behavior
Publisher
Elsevier BV
Published
2009-03
Volume
96
Issue
4-5
Pages
557-567
ISSN
0031-9384 (ISSN-L)
DOI
10.1016/j.physbeh.2008.12.004
OpenAlex
W2044729604
Cited by
246
References
81
Citations by year
2026: 3 · 2025: 8 · 2024: 12 · 2023: 16 · 2022: 18 · 2021: 11 · 2020: 13 · 2019: 16 · 2018: 17 · 2017: 22 · 2016: 21 · 2015: 15 · 2014: 19 · 2013: 18 · 2012: 11
FWCI
3.55
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Physiology & Behavior (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
gut microbiota, dietary intervention, memory, learning behavior, gut microbiota diversity, anxiety, working memory, reference memory, CF1 mice
MeSH terms
Analysis of Variance, Animals, Animal Feed, Association Learning, Bacteria, Colon, Dietary Proteins, Discrimination Learning, Exploratory Behavior, Longitudinal Studies, Male, Memory, Random Allocation, RNA, Bacterial, RNA, Ribosomal, Spatial Behavior, Time Factors, Colony Count, Microbial, Statistics, Nonparametric, DNA, Intergenic, Biodiversity, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review