Cited by 223Alzheimer's disease research and treatmentsNeuroinflammation and Neurodegeneration MechanismsNeurological Disease Mechanisms and Treatments

Intrahippocampal LPS injections reduce Aβ load in APP+PS1 transgenic mice

DiCarlo, G·2001·Neurobiology of Aging

Abstract

Multiple lines of evidence indicate that inflammatory processes are involved in the pathogenesis of Alzheimer's disease. Lipopolysaccharide (LPS) is widely used to induce inflammation in experimental systems. Consequently we injected LPS or saline intrahippocampally in 11 and 16 months old APP+PS1 transgenic mice to induce brain inflammation, then used immunocytochemistry to examine amyloid pathology 7 days later. As expected, LPS activated microglia as indicated by a significant increase in the area covered by major histocompatibility complex-II (MHC-II) immunostaining in the mice injected with LPS compared to the saline injected. Simultaneously, Abeta immunostaining showed an unexpected reduction of the Abeta load in the mice injected with LPS compared to the saline injected. This effect of LPS on the Abeta load in APP+PS1 mice strengthens the hypothesis that moderate amounts of microglial activation may be beneficial in Alzheimer's disease, by increasing the clearance of Abeta.

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Crossref · 10.1016/s0197-4580(01)00292-5

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  • Increased gliosis induced by brain infusion of lipopolysaccharide or overexpression of interleukin-6 or interferon-γ markedly reduces amyloid plaque burden.

    “For example, increased gliosis induced by brain infusion of lipopolysaccharide (4) or overexpression of the inflammatory cytokine interleukin-6 (5) or interferon-γ (6) markedly reduces amyloid plaque burden.”

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Authors
DiCarlo, G.
Affiliations
University of South Florida (US)
Corresponding author
DiCarlo, G
Venue
Neurobiology of Aging
Publisher
Elsevier BV
Published
2001-12
Volume
22
Issue
6
Pages
1007-1012
ISSN
0197-4580 (ISSN-L)
OpenAlex
W1993909442
Cited by
223
References
28
Citations by year
2026: 2 · 2025: 5 · 2024: 7 · 2023: 2 · 2022: 5 · 2021: 5 · 2020: 12 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 12 · 2015: 15 · 2014: 11 · 2013: 8 · 2012: 21
FWCI
4.78
Type
journal article
Language
en
Open access
closed
Locations
  • Neurobiology of Aging (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (R01AG018478, AG 15490, R01AG015490, AG18478)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
lipopolysaccharide, Alzheimer's disease, amyloid-beta, APP/PS1 mice, microglial activation, neuroinflammation, hippocampus, MHC class II, amyloid-beta clearance, intrahippocampal injection
MeSH terms
Animals, Gene Expression Regulation, Genes, MHC Class II, Hippocampus, Immunohistochemistry, Lipopolysaccharides, Membrane Proteins, Mice, Transgenic, Microinjections, Amyloid beta-Peptides, Amyloid beta-Protein Precursor, Microglia, Mice, Presenilin-1

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