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The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans

Tissenbaum, Heidi A., Guarente, Leonard·2001·Nature
DOI 10.1038/35065638
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Abstract

In Caenorhabditis elegans, mutations that reduce the activity of an insulin-like receptor (daf-2) or a phosphatidylinositol-3-OH kinase (age-1) favour entry into the dauer state during larval development and extend lifespan in adults. Downregulation of this pathway activates a forkhead transcription factor (daf-16), which may regulate targets that promote dauer formation in larvae and stress resistance and longevity in adults. In yeast, the SIR2 gene determines the lifespan of mother cells, and adding an extra copy of SIR2 extends lifespan. Sir2 mediates chromatin silencing through a histone deacetylase activity that depends on NAD (nicotinamide adenine dinucleotide) as a cofactor. We have surveyed the lifespan of C. elegans strains containing duplications of chromosomal regions. Here we report that a duplication containing sir-2.1-the C. elegans gene most homologous to yeast SIR2-confers a lifespan that is extended by up to 50%. Genetic analysis indicates that the sir-2.1 transgene functions upstream of daf-16 in the insulin-like signalling pathway. Our findings suggest that Sir2 proteins may couple longevity to nutrient availability in many eukaryotic organisms.

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Crossref · 10.1038/35065638

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4 of 4 references verified0 citation errors · 0 not checkable

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  • Enhanced expression of the worm sir-2.1 and fly dSir2 orthologs could extend lifespan in both invertebrate model systems.

    “and subsequent reports indicated that enhanced expression of the worm (sir-2.1) and fly (dSir2) orthologs could extend lifespan in both invertebrate model systems (Rogina and Helfand, 2004; Tissenbaum and Guarente, 2001).”

    From The Hallmarks of Aging (2013) · cites this paper as Tissenbaum and Guarente, 2001

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Research works printed but not linked: 23

  1. Kimura, K., Tissenbaum, H. A., Liu, Y. & Ruvkun, G. The daf-2 insulin receptor family member regulates longevity and diapause in Caenorhabditis elegans. Science 277, 942–946 (1997). · DOI
  2. Morris, J. Z., Tissenbaum, H. A. & Ruvkun, G. A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature 382, 536–539 (1996). · DOI
  3. Larsen, P. L., Albert, P. S. & Riddle, D. L. Genes that regulate both development and longevity in Caenorhabditis elegans. Genetics 139, 1567–1583 (1995). · DOI
  4. Friedman, D. B. & Johnson, T. E. A mutation in the age-1 gene in Caenorhabditis elegans lengthens life and reduces hermaphrodite fertility. Genetics 118, 75–86 (1988). · DOI
  5. Kenyon, C., Chang, J., Gensch, E., Rudner, A. & Tabtiang, R. A C. elegans mutant that lives twice as long as wild type. Nature 366, 461–464 (1993). · DOI · Paper page
  6. Dorman, J. B., Albinder, B., Shroyer, T. & Kenyon, C. The age-1 and daf-2 genes function in a common pathway to control the lifespan of Caenorhabditis elegans. Genetics 141, 1399–1406 (1995). · DOI
  7. Ogg, S. et al. The Fork head transcription factor DAF-16 transduces insulin-like metabolic and longevity signals in C. elegans. Nature 389, 994–999 (1997). · DOI
  8. Lin, K., Dorman, J. B., Rodan, A. & Kenyon, C. daf-16: An HNF-3/forkhead family member that can function to double the life-span of Caenorhabditis elegans. Science 278, 1319–1322 (1997). · DOI
  9. Lithgow, G. J., White, T. M., Melov, S. & Johnson, T. E. Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc. Natl Acad. Sci. USA 92, 7540–7544 (1995). · DOI · Paper page
  10. Smith, J. S. et al. A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family. Proc. Natl Acad. Sci. USA 97, 6658–6663 (2000). · DOI · Paper page
  11. Landry, J. et al. The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc. Natl Acad. Sci. USA 97, 5807–5811 (2000). · DOI · Paper page
  12. Frye, R. A. Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins. Biochem. Biophys. Res. Comm. 273, 793–798 (2000). · DOI · Paper page
  13. Herman, R. K., Madl, J. E. & Cari, C. K. Duplications in Caenorhabditis elegans. Dev. Biol. 49, 200–219 (1979).
  14. Paradis, S., Ailion, M., Toker, A., Thomas, J. H. & Ruvkun, G. A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans. Genes Dev. 13, 1438–1452 (1999). · DOI
  15. Lakowski, B. & Hekimi, S. Determination of life-span in Caenorhabditis elegans by four clock genes. Science 272, 1010–1013 (1996). · DOI
  16. Gems, D. et al. Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans. Genetics 150, 129–155 (1998). · DOI
  17. Tissenbaum, H. A. & Ruvkun, G. An insulin-like signaling pathway affects both longevity and reproduction in Caenorhabditis elegans. Genetics 148, 703–717 (1998). · DOI
  18. Riddle, D. L. & Albert, P. S. in C. elegans II (eds Riddle, D. L., Blumenthal, T., Meyer, B. J. & Priess, J. R.) 739–768 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1997).
  19. Brenner, S. The genetics of Caenorhabditis elegans. Genetics 77, 71–94 (1974). · DOI
  20. Sulston, J. & Hodgkin, J. in The Nematode Caenorhabditis elegans (ed. Wood, W. B.) 587–602 (Cold Spring Harbor Laboratory, Cold Spring Harbor, 1988).
  21. Mello, C. C., Kramer, J. M., Stinchcomb, D. & Ambros, V. Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J. 10, 3959–3970 (1991). · DOI
  22. Apfeld, J. & Kenyon, C. Cell nonautonomy of C. elegans daf-2 function in the regulation of diapause and lifespan. Cell 95, 199–210 (1998). · DOI
  23. Hedgecock, E. M. & Herman, R. K. The ncl-1 gene and genetic mosaics of Caenorhabditis elegans. Genetics 141, 989–1006 (1995). · DOI

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Metadata

Authors
Tissenbaum, H. A., & Guarente, L.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2001-03
Volume
410
Issue
6825
Pages
227-230
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/35065638
Cited by
1,603
References
4
Language
en
Licence
www.springer.com/tdm (tdm)

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