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I-TASSER: a unified platform for automated protein structure and function prediction

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Roy, Ambrish, Kucukural, Alper, Zhang, Yang·2010·Nature Protocols
DOI 10.1038/nprot.2010.5
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Abstract

The iterative threading assembly refinement (I-TASSER) server is an integrated platform for automated protein structure and function prediction based on the sequence-to-structure-to-function paradigm. Starting from an amino acid sequence, I-TASSER first generates three-dimensional (3D) atomic models from multiple threading alignments and iterative structural assembly simulations. The function of the protein is then inferred by structurally matching the 3D models with other known proteins. The output from a typical server run contains full-length secondary and tertiary structure predictions, and functional annotations on ligand-binding sites, Enzyme Commission numbers and Gene Ontology terms. An estimate of accuracy of the predictions is provided based on the confidence score of the modeling. This protocol provides new insights and guidelines for designing of online server systems for the state-of-the-art protein structure and function predictions. The server is available at http://zhanglab.ccmb.med.umich.edu/I-TASSER.

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Cited by

Metadata

Authors
Roy, A., Kucukural, A., & Zhang, Y.
Venue
Nature Protocols
Publisher
Springer Science and Business Media LLC
Published
2010-03-25
Volume
5
Issue
4
Pages
725-738
ISSN
  • 1750-2799 (ISSN-L)
  • 1754-2189
DOI
10.1038/nprot.2010.5
OpenAlex
W2037312364
Cited by
6,170
References
7
FWCI
116.26
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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