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Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins
Abstract
Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains (μ 1 and μ 2 ) of two clathrin-associated protein complexes (AP-1 and AP-2, respectively) specifically interacted with tyrosine-based signals of several integral membrane proteins. The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery.
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Metadata
- Authors
- Ohno, H., Stewart, J., Fournier, M.-C., Bosshart, H., Rhee, I., Miyatake, S., Saito, T., Gallusser, A., Kirchhausen, T., & Bonifacino, J. S.
- Venue
- Science
- Published
- 1995-09-29
- Volume
- 269
- Issue
- 5232
- Pages
- 1872-1875
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1979815209
- Cited by
- 969
- References
- 1
- FWCI
- 26.75
- Type
- journal article
- Language
- en
- Open access
- closed
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