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Mechanisms of Alternative Pre-Messenger RNA Splicing
Open accessAbstract
▪ Abstract Alternative pre-mRNA splicing is a central mode of genetic regulation in higher eukaryotes. Variability in splicing patterns is a major source of protein diversity from the genome. In this review, I describe what is currently known of the molecular mechanisms that control changes in splice site choice. I start with the best-characterized systems from the Drosophila sex determination pathway, and then describe the regulators of other systems about whose mechanisms there is some data. How these regulators are combined into complex systems of tissue-specific splicing is discussed. In conclusion, very recent studies are presented that point to new directions for understanding alternative splicing and its mechanisms.
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“U2 snRNA binds to the branch point during spliceosome assembly and then forms base pairs with the U6 snRNA to become a key portion of the spliceosome catalytic centre for all major class introns6,8,183,184”
From The neurogenetics of alternative splicing (2016) · cites this paper as [184]
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“However, the molecular mechanism by which tissue-specific alternative splicing is regulated remains largely unknown, except for a few well-characterized examples (Smith and Valcarcel, 2000; Maniatis and Tasic, 2002; Black, 2003).”
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Metadata
- Authors
- Black, D. L.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Black, Douglas L.
- ORCID
- Black, Douglas L.
- Venue
- Annual Review of Biochemistry
- Publisher
- Annual Reviews
- Published
- 2003-06
- Volume
- 72
- Issue
- 1
- Pages
- 291-336
- ISSN
- 0066-4154 (ISSN-L)
- 1545-4509
- OpenAlex
- W2109936378
- Cited by
- 2,704
- References
- 377
- Citations by year
- 2026: 69 · 2025: 76 · 2024: 92 · 2023: 83 · 2022: 79 · 2021: 91 · 2020: 90 · 2019: 103 · 2018: 103 · 2017: 99 · 2016: 108 · 2015: 130 · 2014: 121 · 2013: 153 · 2012: 139
- FWCI
- 10.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Annual Review of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- alternative splicing, splicing regulation, tissue-specific splicing, protein diversity, splicing regulators, genetic control
- MeSH terms
- Animals, Drosophila, Exons, Introns, Organ Specificity, RNA Precursors, RNA, Messenger, Transcription Factors, RNA-Binding Proteins, Spliceosomes, Alternative Splicing, Sex Determination Processes
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