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Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii

Li, Yantao, Han, Danxiang, Hu, Guangrong, Sommerfeld, Milton, Hu, Qiang·2010·Biotechnology and Bioengineering

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Abstract

Starch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP‐glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8‐ and 3.5‐fold higher, respectively, than the wild‐type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation. Biotechnol. Bioeng. 2010;107: 258–268. © 2010 Wiley Periodicals, Inc.

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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Biodiesel from microalgae
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524
Chlorophyll fluorescence—a practical guide
Maxwell, K., & Johnson, G. N. (2000) · Journal of Experimental Botany · doi:10.1093/jexbot/51.345.659 · Cited by 9,347
Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879
Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans
Li, Y., Horsman, M., Wang, B., Wu, N., & Lan, C. Q. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1681-1 · Cited by 1,116
Self-citation
Increase in Chlorella strains calorific values when grown in low nitrogen medium
Illman, A. M., Scragg, A. H., & Shales, S. W. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00266-0 · Cited by 1,008
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681
Self-citation
A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution
Rosenberg, J. N., Oyler, G. A., Wilkinson, L., & Betenbaugh, M. J. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.07.008 · Cited by 631

Show all 8 references

Research works printed but not linked: 42

10.1007/978-1-4020-3218-9_3 · DOI · Paper page
10.1146/annurev.arplant.54.031902.134927 · DOI · Paper page
10.1007/BF00194509 · DOI
10.1046/j.1432-1327.2000.00961.x · DOI · Paper page
10.1016/0005-2728(81)90113-4 · DOI · Paper page
10.1007/s10142-007-0070-7 · doi:10.1007/s10142-007-0070-7
10.1104/pp.120.4.993 · DOI
10.1007/BF00303649 · doi:10.1007/bf00303649
10.1007/BF02941703 · DOI · Paper page
10.1111/j.1365-313X.2008.03442.x · DOI · Paper page
10.5962/bhl.title.5893 · DOI
10.1016/S0065-2296(08)60238-4 · DOI · Paper page
10.1111/j.1365-313X.2008.03492.x · DOI · Paper page
10.1104/pp.126.2.861 · DOI · Paper page
10.1007/s00425-004-1236-3 · DOI
10.1016/j.ymben.2010.02.002 · DOI · Paper page
10.1007/s10529-009-9975-7 · DOI · Paper page
10.1007/s00425-002-0810-9 · doi:10.1007/s00425-002-0810-9
10.1093/jxb/erg020 · DOI
10.1016/j.copbio.2007.02.001 · DOI · Paper page
Mouille G, 1996, Preamylopectin processing: A mandatory step for starch biosynthesis in plants, Plant Cell, 8, 1353, 10.2307/3870306 · DOI
10.1104/pp.125.4.1558 · DOI · Paper page
Murphy D, 2004, Plant lipids: Biology, utilisation and manipulation
10.1104/pp.122.4.1193 · DOI
10.1016/0168-9452(91)90135-U · doi:10.1016/0168-9452(91)90135-u
10.1016/S0163-7827(01)00023-6 · DOI · Paper page
10.1111/j.0022-3646.1994.00972.x · DOI · Paper page
10.1104/pp.113.1.75 · DOI · Paper page
SheehanJ DunahayT BenemannJ RoesslerPG.1998.US Department of Energy's Office of Fuels Development July 1998. A Look Back at the US Department of Energy's Aquatic Species Program—Biodiesel from Algae Close Out Report TP‐580‐24190. Golden CO: National Renewable Energy Laboratory. · DOI · Paper page
10.1016/S0098-8472(96)01031-3 · DOI
10.1074/jbc.271.27.16281 · DOI
10.1104/pp.104.046854 · DOI
10.1111/j.1467-7652.2007.00252.x · DOI · Paper page
10.1128/EC.00272-09 · DOI · Paper page
10.1104/pp.102.016527 · doi:10.1104/pp.102.016527
10.1016/S0176-1617(11)81192-2 · DOI · Paper page
10.1093/jxb/ern206 · DOI · Paper page
10.1016/j.biotechadv.2009.07.001 · DOI · Paper page
Yamaberi K, 1998, Nitrogen depletion for intracellular triglyceride accumulation to enhance liquefaction yield of marine microalgal cells into a fuel oil, J Mar Biotechnol, 6, 44
10.1128/JB.183.3.1069-1077.2001 · DOI · Paper page
10.1038/ng.85 · DOI · Paper page
10.1105/tpc.9.6.909 · DOI · Paper page

Microalgae biofuels: A critical review of issues, problems and the way forward
Lam, M. K., & Lee, K. T. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.11.008 · Cited by 936
Microalgae-based carbohydrates for biofuel production
Chen, C.-Y., Zhao, X.-Q., Yen, H.-W., Ho, S.-H., Cheng, C.-L., Lee, D.-J., Bai, F.-W., & Chang, J.-S. (2013) · Biochemical Engineering Journal · doi:10.1016/j.bej.2013.03.006 · Cited by 724
The lipid biochemistry of eukaryotic algae
Li-Beisson, Y., Thelen, J. J., Fedosejevs, E., & Harwood, J. L. (2019) · Progress in Lipid Research · doi:10.1016/j.plipres.2019.01.003 · Cited by 453
Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas
Boyle, N. R., Page, M. D., Liu, B., Blaby, I. K., Casero, D., Kropat, J., Cokus, S. J., Hong-Hermesdorf, A., Shaw, J., Karpowicz, S. J., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.334052 · Cited by 419
Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae
Li, J., Han, D., Wang, D., Ning, K., Jia, J., Wei, L., Jing, X., Huang, S., Chen, J., Li, Y., et al. (2014) · The Plant Cell · doi:10.1105/tpc.113.121418 · Cited by 412
Self-citation
Current status and challenges on microalgae-based carbon capture
Lam, M. K., Lee, K. T., & Mohamed, A. R. (2012) · International Journal of Greenhouse Gas Control · doi:10.1016/j.ijggc.2012.07.010 · Cited by 393
Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169
Msanne, J., Xu, D., Konda, A. R., Casas-Mollano, J. A., Awada, T., Cahoon, E. B., & Cerutti, H. (2012) · Phytochemistry · doi:10.1016/j.phytochem.2011.12.007 · Cited by 391
Phospholipid:Diacylglycerol Acyltransferase Is a Multifunctional Enzyme Involved in Membrane Lipid Turnover and Degradation While Synthesizing Triacylglycerol in the Unicellular Green Microalga Chlamydomonas reinhardtii
Yoon, K., Han, D., Li, Y., Sommerfeld, M., & Hu, Q. (2012) · The Plant Cell · doi:10.1105/tpc.112.100701 · Cited by 377
Self-citation
Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: A review
Khoo, K. S., Ahmad, I., Chew, K. W., Iwamoto, K., Bhatnagar, A., & Show, P. L. (2023) · Progress in Energy and Combustion Science · doi:10.1016/j.pecs.2023.101071 · Cited by 363
Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
Work, V. H., Radakovits, R., Jinkerson, R. E., Meuser, J. E., Elliott, L. G., Vinyard, D. J., Laurens, L. M. L., Dismukes, G. C., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00075-10 · Cited by 353
A chloroplast pathway for the de novo biosynthesis of triacylglycerol in Chlamydomonas reinhardtii
Fan, J., Andre, C., & Xu, C. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.018 · Cited by 347
Trends and novel strategies for enhancing lipid accumulation and quality in microalgae
Singh, P., Kumari, S., Guldhe, A., Misra, R., Rawat, I., & Bux, F. (2016) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.11.001 · Cited by 309
Flow cytometry for the development of biotechnological processes with microalgae
Hyka, P., Lickova, S., Přibyl, P., Melzoch, K., & Kovar, K. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.04.007 · Cited by 286
Accumulation of energy reserves in algae: From cell cycles to biotechnological applications
Vitova, M., Bisova, K., Kawano, S., & Zachleder, V. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.04.012 · Cited by 283
Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production
Ma, Y., Wang, Z., Yu, C., Yin, Y., & Zhou, G. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.06.047 · Cited by 263
Advances in the Production of High-Value Products by Microalgae
Leu, S., & Boussiba, S. (2014) · Industrial Biotechnology · doi:10.1089/ind.2013.0039 · Cited by 253
Strategies for Lipid Production Improvement in Microalgae as a Biodiesel Feedstock
Zhu, L. D., Li, Z. H., & Hiltunen, E. (2016) · BioMed Research International · doi:10.1155/2016/8792548 · Cited by 245
Genetic Engineering of the Calvin Cycle toward Enhanced Photosynthetic CO2 Fixation in Microalgae
Yang, B., Liu, J., Ma, X., Guo, B., Liu, B., Wu, T., Jiang, Y., & Chen, F. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0916-8 · Cited by 231
Current trends to comprehend lipid metabolism in diatoms
Zulu, N. N., Zienkiewicz, K., Vollheyde, K., & Feussner, I. (2018) · Progress in Lipid Research · doi:10.1016/j.plipres.2018.03.001 · Cited by 226
Storage of starch and lipids in microalgae: Biosynthesis and manipulation by nutrients
Ran, W., Wang, H., Liu, Y., Qi, M., Xiang, Q., Yao, C., Zhang, Y., & Lan, X. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.121894 · Cited by 224
Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation
Yao, C., Ai, J., Cao, X., Xue, S., & Zhang, W. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.030 · Cited by 213
Biochemical activities in Chlorella sp. and Nannochloropsis salina during lipid and sugar synthesis in a lab-scale open pond simulating reactor
Bellou, S., & Aggelis, G. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.01.010 · Cited by 189
Stresses as First-Line Tools for Enhancing Lipid and Carotenoid Production in Microalgae
Shi, T.-Q., Wang, L.-R., Zhang, Z.-X., Sun, X.-M., & Huang, H. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00610 · Cited by 182
Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production
Shin, Y. S., Jeong, J., Nguyen, T. H. T., Kim, J. Y. H., Jin, E., & Sim, S. J. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2018.09.121 · Cited by 179
Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery
Dong, H.-P., Williams, E., Wang, D.-Z., Xie, Z.-X., Hsia, R.-C., Jenck, A., Halden, R., Li, J., Chen, F., & Place, A. R. (2013) · Plant Physiology · doi:10.1104/pp.113.214320 · Cited by 173
“Also, overproduction of neutral lipids in a mutant of C. reinhardtii under nitrogen starvation has been achieved through blocking a competing pathway to produce starch (Li et al., 2010).”
Background
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Wang, H., Alvarez, S., & Hicks, L. M. (2012) · Journal of Proteome Research · doi:10.1021/pr2008225 · Cited by 142
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Authors: Li, Y., Han, D., Hu, G., Sommerfeld, M., & Hu, Q.
Venue: Biotechnology and Bioengineering
Publisher: Wiley
Published: 2010-08-02
Volume: 107
Issue: 2
Pages: 258-268
ISSN: 0006-3592 (ISSN-L)
1097-0290
DOI: 10.1002/bit.22807
OpenAlex: W2076019237
Cited by: 429
References: 8
FWCI: 13.19
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)

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Inhibition of starch synthesis results in overproduction of lipids in Chlamydomonas reinhardtii

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