The DOI matches its Crossref record; 54 of the 55 references it cites were found in a registry or an index.
Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA
Open accessAbstract
Clinical application of induced pluripotent stem cells (iPSCs) is limited by the low efficiency of iPSC derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover, safe and effective means of directing the fate of patient-specific iPSCs toward clinically useful cell types are lacking. Here we describe a simple, nonintegrating strategy for reprogramming cell fate based on administration of synthetic mRNA modified to overcome innate antiviral responses. We show that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols. We further show that the same technology can be used to efficiently direct the differentiation of RNA-induced pluripotent stem cells (RiPSCs) into terminally differentiated myogenic cells. This technology represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Using enhanced green fluorescent protein mRNA, Rossi and colleagues confirmed that nucleoside modifications can strongly enhance protein expression and suppress cytokine secretion.”
From Sequence-engineered mRNA without chemical nucleoside modifications enables an effective protein therapy in large animals (2015) · cites this paper as [24]
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Our work principally confirms that conventional unmodified mRNAs are inferior to molecules harboring specific nucleoside modifications as suggested by various recent reports,23,24,25,26,27 however, the picture changes dramatically with a sophisticated sequence-engineering.”
From Sequence-engineered mRNA without chemical nucleoside modifications enables an effective protein therapy in large animals (2015) · cites this paper as [24]
The cited source says what the claim attributes to it.
In the citing paper
“Second, we studied the efficiency and robustness of cellular reprogramming of human fibroblasts to iPSCs with mRNA-encoded transcription factors, which is considered to benefit from prolonged mRNA stability.17,51”
From Improving mRNA-Based Therapeutic Gene Delivery by Expression-Augmenting 3′ UTRs Identified by Cellular Library Screening (2019) · cites this paper as [51]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA
Diebold, S. S., Kaisho, T., Hemmi, H., Akira, S., & Reis e Sousa, C. (2004) · Science · doi:10.1126/science.1093616 · Cited by 3,444
Expression of a single transfected cDNA converts fibroblasts to myoblasts
Davis, R. L., Weintraub, H. M., & Lassar, A. B. (1987) · Cell · doi:10.1016/0092-8674(87)90585-x · Cited by 3,388
Karikó, K., Buckstein, M., Ni, H., & Weissman, D. (2005) · Immunity · doi:10.1016/j.immuni.2005.06.008 · Cited by 2,728
5'-Triphosphate RNA Is the Ligand for RIG-I
Hornung, V., Ellegast, J., Kim, S., Brzózka, K., Jung, A., Kato, H., Poeck, H., Akira, S., Conzelmann, K.-K., Schlee, M., et al. (2006) · Science · doi:10.1126/science.1132505 · Cited by 2,474
Reprogramming of human somatic cells to pluripotency with defined factors
Park, I.-H., Zhao, R., West, J. A., Yabuuchi, A., Huo, H., Ince, T. A., Lerou, P. H., Lensch, M. W., & Daley, G. Q. (2008) · Nature · doi:10.1038/nature06534 · Cited by 2,436
Self-citationHuman induced pluripotent stem cells free of vector and transgene sequences
Yu, J., Hu, K., Smuga-Otto, K., Tian, S., Stewart, R., Slukvin, I. I., & Thomson, J. A. (2009) · Science · doi:10.1126/science.1172482 · Cited by 2,386
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates
Pichlmair, A., Schulz, O., Tan, C. P., Näslund, T. I., Liljeström, P., Weber, F., & Reis e Sousa, C. (2006) · Science · doi:10.1126/science.1132998 · Cited by 2,210
Cited by
Sorted by citations
Lipid nanoparticles for mRNA delivery
Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00358-0 · Cited by 4,076
mRNA-based therapeutics--developing a new class of drugs
Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474
A more efficient method to generate integration-free human iPS cells
Okita, K., Matsumura, Y., Sato, Y., Okada, A., Morizane, A., Okamoto, S., Hong, H., Nakagawa, M., Tanabe, K., Tezuka, K.-I., et al. (2011) · Nature Methods · doi:10.1038/nmeth.1591 · Cited by 2,061
Induced pluripotent stem cell technology: a decade of progress.
Shi, Y., Inoue, H., Wu, J. C., & Yamanaka, S. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.245 · Cited by 1,525
Somatic coding mutations in human induced pluripotent stem cells
Gore, A., Li, Z., Fung, H.-L., Young, J. E., Agarwal, S., Antosiewicz-Bourget, J., Canto, I., Giorgetti, A., Israel, M. A., Kiskinis, E., et al. (2011) · Nature · doi:10.1038/nature09805 · Cited by 1,273
Self-citationLaflamme, M. A., & Murry, C. E. (2011) · Nature · doi:10.1038/nature10147 · Cited by 1,272
Highly Efficient miRNA-Mediated Reprogramming of Mouse and Human Somatic Cells to Pluripotency
Anokye‐Danso, F., Trivedi, C. M., Juhr, D., Gupta, M., Cui, Z., Tian, Y., Zhang, Y., Yang, W., Gruber, P. J., Epstein, J. A., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2011.03.001 · Cited by 1,244
Gene therapy clinical trials worldwide to 2012 – an update
Ginn, S. L., Alexander, I. E., Edelstein, M. L., Abedi, M. R., & Wixon, J. (2013) · The Journal of Gene Medicine · doi:10.1002/jgm.2698 · Cited by 1,187
Heart repair by reprogramming non-myocytes with cardiac transcription factors
Song, K., Nam, Y., Luo, X., Qi, X., Tan, W. H., Huang, G. N., Acharya, A., Smith, C. L., Tallquist, M. D., Neilson, E. G., et al. (2012) · Nature · doi:10.1038/nature11139 · Cited by 1,162
The promise of induced pluripotent stem cells in research and therapy
Robinton, D. A., & Daley, G. Q. (2012) · Nature · doi:10.1038/nature10761 · Cited by 1,150
Self-citationWidespread occurrence of 5-methylcytosine in human coding and non-coding RNA
Squires, J. E., Patel, H. R., Nousch, M., Sibbritt, T., Humphreys, D. T., Parker, B. J., Suter, C. M., & Preiß, T. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks144 · Cited by 1,033
Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z. D., Ziller, M., Croft, G. F., Amoroso, M. W., Oakley, D. H., et al. (2011) · Cell · doi:10.1016/j.cell.2010.12.032 · Cited by 1,009
Self-citationCopy number variation and selection during reprogramming to pluripotency
Hussein, S. M. I., Batada, N. N., Vuoristo, S., Ching, R. W., Autio, R., Närvä, E., Ng, S. H. S., Sourour, M., Hämäläinen, R. H., Olsson, C., et al. (2011) · Nature · doi:10.1038/nature09871 · Cited by 980
RNA N 6 -methyladenosine methylation in post-transcriptional gene expression regulationPreprint
Yue, Y., Liu, J., & He, C. (2015) · Genes & Development · doi:10.1101/gad.262766.115 · Cited by 937
Gene therapy clinical trials worldwide to 2017: An update
Ginn, S. L., Amaya, A. K., Alexander, I. E., Edelstein, M., & Abedi, M. R. (2018) · The Journal of Gene Medicine · doi:10.1002/jgm.3015 · Cited by 903
Karikó, K., Muramatsu, H., Ludwig, J., & Weissman, D. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr695 · Cited by 898
mRNA capping: biological functions and applications
Ramanathan, A., Robb, G. B., & Chan, S. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw551 · Cited by 961
The tumorigenicity of human embryonic and induced pluripotent stem cells
Ben-David, U., & Benvenisty, N. (2011) · Nature Reviews Cancer · doi:10.1038/nrc3034 · Cited by 889
In vitro and ex vivo strategies for intracellular delivery
Stewart, M. P., Sharei, A., Ding, X., Sahay, G., Langer, R., & Jensen, K. F. (2016) · Nature · doi:10.1038/nature19764 · Cited by 885
A decade of transcription factor-mediated reprogramming to pluripotency
Takahashi, K., & Yamanaka, S. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.8 · Cited by 894
Emerging trends and new developments in regenerative medicine: a scientometric update (2000 – 2014)
Chen, C., Dubin, R., & Kim, M. C. (2014) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2014.920813 · Cited by 875
Induced Pluripotent Stem Cells: Past, Present, and Future
Yamanaka, S. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.05.005 · Cited by 878
Wang, G., McCain, M. L., Yang, L., He, A., Pasqualini, F. S., Agarwal, A., Yuan, H., Jiang, D., Zhang, D., Zangi, L., et al. (2014) · Nature Medicine · doi:10.1038/nm.3545 · Cited by 837
Induced pluripotency: history, mechanisms, and applicationsPreprint
Stadtfeld, M., & Hochedlinger, K. (2010) · Genes & Development · doi:10.1101/gad.1963910 · Cited by 857
Knocking down disease: a progress report on siRNA therapeutics
Wittrup, A., & Lieberman, J. (2015) · Nature Reviews Genetics · doi:10.1038/nrg3978 · Cited by 827
Tools for translation: non-viral materials for therapeutic mRNA delivery
Hajj, K. A., & Whitehead, K. A. (2017) · Nature Reviews Materials · doi:10.1038/natrevmats.2017.56 · Cited by 799
Sellgren, C. M., Gracias, J., Watmuff, B., Biag, J. D., Thanos, J. M., Whittredge, P. B., Fu, T., Worringer, K. A., Brown, H. E., Wang, J. P., et al. (2019) · Nature Neuroscience · doi:10.1038/s41593-018-0334-7 · Cited by 810
Human iPSC-based modeling of late-onset disease via progerin-induced aging
Miller, J. D., Ganat, Y. M., Kishinevsky, S., Bowman, R. L., Liu, B., Tu, E. Y., Mandal, P. K., Vera, E., Shim, J.-W., Kriks, S., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.11.006 · Cited by 777
Self-citationReprogramming of Mouse and Human Cells to Pluripotency Using Mature MicroRNAs
Miyoshi, N., Ishii, H., Nagano, H., Haraguchi, N., Dewi, D. L., Kano, Y., Nishikawa, S., Tanemura, M., Mimori, K., Tanaka, F., et al. (2011) · Cell stem cell · doi:10.1016/j.stem.2011.05.001 · Cited by 756
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 767
Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
Kormann, M. S. D., Hasenpusch, G., Aneja, M. K., Nica, G., Flemmer, A. W., Herber-Jonat, S., Huppmann, M., Mays, L. E., Illenyi, M., Schams, A., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1733 · Cited by 733
Tumorigenicity as a clinical hurdle for pluripotent stem cell therapies
Lee, A. S., Tang, C. Z., Rao, M. S., Weissman, I. L., & Wu, J. C. (2013) · Nature Medicine · doi:10.1038/nm.3267 · Cited by 736
The epitranscriptome beyond m6A
Wiener, D., & Schwartz, S. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-020-00295-8 · Cited by 733
Andries, O., Mc Cafferty, S., De Smedt, S. C., Weiss, R., Sanders, N. N., & Kitada, T. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.051 · Cited by 701
Gene regulation in the immune system by long noncoding RNAs
Chen, Y. G., Satpathy, A. T., & Chang, H. Y. (2017) · Nature Immunology · doi:10.1038/ni.3771 · Cited by 694
Bright and stable near-infrared fluorescent protein for in vivo imaging
Filonov, G. S., Piatkevich, K. D., Ting, L.-M., Zhang, J., Kim, K., & Verkhusha, V. V. (2011) · Nature Biotechnology · doi:10.1038/nbt.1918 · Cited by 682
Zangi, L., Lui, K. O., von Gise, A., Ma, Q., Ebina, W., Ptaszek, L. M., Später, D., Xu, H., Tabebordbar, M., Gorbatov, R., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2682 · Cited by 646
Self-citationThe RNA-Editing Enzyme ADAR1 Controls Innate Immune Responses to RNA
Mannion, N., Greenwood, S. M., Young, R., Cox, S. L., Brindle, J., Read, D. F., Nellåker, C., Vesely, C., Ponting, C. P., McLaughlin, P. J., et al. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.10.041 · Cited by 644
Bahl, K., Senn, J. J., Yuzhakov, O., Bulychev, A., Brito, L. A., Hassett, K. J., Laska, M. E., Smith, M., Almarsson, Ö., Thompson, J., et al. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.03.035 · Cited by 642
Roberts, L. D., Boström, P., O’Sullivan, J. F., Schinzel, R. T., Lewis, G. D., Dejam, A., Lee, Y.-K., Palma, M. J., Calhoun, S., Georgiadi, A., et al. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2013.12.003 · Cited by 643
Self-citationGeneration of vascular endothelial and smooth muscle cells from human pluripotent stem cells
Patsch, C., Meylan, L. C., Thoma, E., Urich, E., Heckel, T., O’Sullivan, J., Grainger, S. J., Kapp, F., Sun, L., Christensen, K., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3205 · Cited by 643
Self-citationDeveloping mRNA-vaccine technologies
Schlake, T., Thess, A., Fotin-Mleczek, M., & Kallen, K.-J. (2012) · RNA Biology · doi:10.4161/rna.22269 · Cited by 622
Unlocking the promise of mRNA therapeutics
Rohner, E., Yang, R., Foo, K. S., Goedel, A., & Chien, K. R. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01491-z · Cited by 612
Ban, H., Nishishita, N., Fusaki, N., Tabata, T., Saeki, K., Shikamura, M., Takada, N., Inoue, M., Hasegawa, M., Kawamata, S., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103509108 · Cited by 628
Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells
Ohi, Y., Qin, H., Hong, C., Blouin, L., Polo, J. M., Guo, T., Qi, Z., Downey, S. L., Manos, P. D., Rossi, D. J., et al. (2011) · Nature Cell Biology · doi:10.1038/ncb2239 · Cited by 609
Self-citationDirect reprogramming of mouse fibroblasts to neural progenitors
Kim, J., Efe, J. A., Zhu, S., Talantova, M. V., Xu, Y., Wang, S., Lipton, S. A., Zhang, K., & Ding, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103113108 · Cited by 580
Efficient generation of lung and airway epithelial cells from human pluripotent stem cells
Huang, S. X. L., Islam, M. N., O’Neill, J. D., Hu, Z., Yang, Y., Chen, Y., Mumau, M. D., Green, M. D., Vunjak‐Novakovic, G., Bhattacharya, J., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2754 · Cited by 565
The tangled history of mRNA vaccines
Dolgin, E. (2021) · Nature · doi:10.1038/d41586-021-02483-w · Cited by 578
Nori, S., Okada, Y., Yasuda, A., Tsuji, O., Takahashi, Y., Kobayashi, Y., Fujiyoshi, K., Koike, M., Uchiyama, Y., Ikeda, E., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1108077108 · Cited by 546
Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications
Liu, G., David, B. T., Trawczynski, M., & Fessler, R. G. (2019) · Stem Cell Reviews and Reports · doi:10.1007/s12015-019-09935-x · Cited by 557
Deterministic direct reprogramming of somatic cells to pluripotency
Rais, Y., Zviran, A., Geula, S., Gafni, O., Chomsky, E., Viukov, S., Mansour, A. A., Caspi, I., Krupalnik, V., Zerbib, M., et al. (2013) · Nature · doi:10.1038/nature12587 · Cited by 522
Kim, J., Eygeris, Y., Gupta, M., & Sahay, G. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.12.014 · Cited by 514
Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors
Han, D., Tàpia, N., Hermann, A., Hemmer, K., Höing, S., Araúzo-Bravo, M. J., Zaehres, H., Wu, G., Frank, S., Moritz, S., et al. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.02.021 · Cited by 524
Thess, A., Grund, S., Mui, B. L., Hope, M. J., Baumhof, P., Fotin-Mleczek, M., & Schlake, T. (2015) · Molecular Therapy · doi:10.1038/mt.2015.103 · Cited by 513
A comparison of non-integrating reprogramming methods
Schlaeger, T. M., Daheron, L. M., Brickler, T., Entwisle, S. W., Chan, K., Cianci, A. L., DeVine, A. L., Ettenger, A., Fitzgerald, K. N., Godfrey, M., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.3070 · Cited by 525
Self-citationMethods for making induced pluripotent stem cells: reprogramming à la carte
Gonzãlez, F., Boué, S., & Belmonte, J. C. I. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2937 · Cited by 542
A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models
Ding, Q., Lee, Y., Schaefer, E. A. K., Peters, D., Veres, A., Kim, K. J., Kuperwasser, N., Motola, D., Meissner, T. B., Hendriks, W. T. J., et al. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.11.011 · Cited by 515
Self-citationInduced Pluripotent Stem Cells Meet Genome Editing
Hockemeyer, D., & Jaenisch, R. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.04.013 · Cited by 499
“…Woltjen et al., 2009), by avoiding integration of the reprogramming factors all together using non-integrating viruses (Fusaki et al., 2009), episomal vectors (Yu et al., 2009) or direct transfection of the reprogramming factors as either mRNA (Warren et al., 2010) or protein (Kim et al., 2009).”
BackgroundDirect conversion of fibroblasts into stably expandable neural stem cells
Thier, M., Wörsdörfer, P., Lakes, Y. B., Gorris, R., Herms, S., Opitz, T., Seiferling, D., Quandel, T., Hoffmann, P., Nöthen, M. M., et al. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.03.003 · Cited by 447
Chou, B.-K., Mali, P., Huang, X., Ye, Z., Dowey, S. N., Resar, L. M. S., Zou, C., Zhang, Y. A., Tong, J., & Cheng, L. (2011) · Cell Research · doi:10.1038/cr.2011.12 · Cited by 482
Darabi, R., Arpke, R. W., Irion, S., Dimos, J. T., Grskovic, M., Kyba, M., & Perlingeiro, R. C. R. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.02.015 · Cited by 481
Sensing Self and Foreign Circular RNAs by Intron Identity
Chen, Y. G., Kim, M. V., Chen, X., Batista, P. J., Aoyama, S., Wilusz, J. E., Iwasaki, A., & Chang, H. Y. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.05.022 · Cited by 475
Induced pluripotent stem cells — opportunities for disease modelling and drug discovery
Grs̆ković, M., Javaherian, A., Strulovici, B., & Daley, G. Q. (2011) · Nature Reviews Drug Discovery · doi:10.1038/nrd3577 · Cited by 492
Self-citationModifications in an Emergency: The Role of N1-Methylpseudouridine in COVID-19 Vaccines
Nance, K. D., & Meier, J. L. (2021) · ACS Central Science · doi:10.1021/acscentsci.1c00197 · Cited by 466
Pluripotent stem cells in regenerative medicine: challenges and recent progress
Tabar, V. S., & Studer, L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3563 · Cited by 467
Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications
Cerneckis, J., Cai, H., & Shi, Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01809-0 · Cited by 452
Induced pluripotent stem cells: the new patient?
Bellin, M., Marchetto, M. C., Gage, F. H., & Mummery, C. L. (2012) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3448 · Cited by 454
Generation of induced pluripotent stem cells from urine
Zhou, T., Benda, C., Duzinger, S., Huang, Y., Li, X., Li, Y., Guo, X., Cao, G., Chen, S., Hao, L., et al. (2011) · Journal of the American Society of Nephrology · doi:10.1681/asn.2011010106 · Cited by 421
Singh, V. K., Kalsan, M., Kumar, N., Saini, A., & Chandra, R. (2015) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2015.00002 · Cited by 442
Burridge, P. W., Thompson, S. A., Millrod, M. A., Weinberg, S. H., Yuan, X., Peters, A., Mahairaki, V., Koliatsos, V. E., Tung, L., & Zambidis, E. T. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018293 · Cited by 432
The challenge and prospect of mRNA therapeutics landscape
Weng, Y., Li, C., Yang, T., Hu, B., Zhang, M., Guo, S., Xiao, H., Liang, X.-J., & Huang, Y. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107534 · Cited by 417
Steps Toward Safe Cell Therapy Using Induced Pluripotent Stem Cells
Okano, H., Nakamura, M., Yoshida, K., Okada, Y., Tsuji, O., Nori, S., Ikeda, E., Yamanaka, S., & Miura, K. (2013) · Circulation Research · doi:10.1161/circresaha.111.256149 · Cited by 425
Synthetic Biology Moving into the Clinic
Ruder, W. C., Lu, T., & Collins, J. J. (2011) · Science · doi:10.1126/science.1206843 · Cited by 410
Self-citationChen, J., Huang, Z., Seok, H., Ding, J., Kataoka, M., Zhang, Z., Hu, X., Wang, G., Lin, Z., Wang, S., et al. (2013) · Circulation Research · doi:10.1161/circresaha.112.300658 · Cited by 403
Liang, F., Lindgren, G., Lin, A., Thompson, E. A., Ols, S., Röhss, J., John, S., Hassett, K., Yuzhakov, O., Bahl, K., et al. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2017.08.006 · Cited by 395
New Modalities for Challenging Targets in Drug Discovery
Valeur, E., Guéret, S. M., Adihou, H., Gopalakrishnan, R., Lemurell, M., Waldmann, H., Grossmann, T. N., & Plowright, A. T. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201611914 · Cited by 394
Synthetic Biology and Personalized Medicine
Jain, K. (2012) · Medical Principles and Practice · doi:10.1159/000341794 · Cited by 380
Stem cells in dentistry – Part I: Stem cell sources
Egusa, H., Sonoyama, W., Nishimura, M., Atsuta, I., & Akiyama, K. (2012) · Journal of Prosthodontic Research · doi:10.1016/j.jpor.2012.06.001 · Cited by 387
Development of Defective and Persistent Sendai Virus Vector
Nishimura, K., Sano, M., Ohtaka, M., Furuta, B., Umemura, Y., Nakajima, Y., Ikehara, Y., Kobayashi, T., Segawa, H., Takayasu, S., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.183780 · Cited by 368
Induced Pluripotent Stem Cells and Their Use in Human Models of Disease and Development
Karagiannis, P., Takahashi, K., Saito, M. K., Yoshida, Y., Okita, K., Watanabe, A., Inoue, H., Yamashita, J. K., Todani, M., Nakagawa, M., et al. (2018) · Physiological Reviews · doi:10.1152/physrev.00039.2017 · Cited by 391
Orlandini von Niessen, A. G., Poleganov, M. A., Rechner, C., Plaschke, A., Kranz, L. M., Fesser, S., Diken, M., Löwer, M., Vallazza, B., Beissert, T., et al. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2018.12.011 · Cited by 365
A Review of the Methods for Human iPSC Derivation
Malik, N., & Rao, M. S. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-348-0_3 · Cited by 382
Bioengineering human myocardium on native extracellular matrix
Guyette, J. P., Charest, J. M., Mills, R. W., Jank, B. J., Moser, P. T., Gilpin, S. E., Gershlak, J. R., Okamoto, T., Gonzalez, G., Milan, D. J., et al. (2016) · Circulation Research · doi:10.1161/circresaha.115.306874 · Cited by 343
Realistic control of network dynamics
Cornelius, S. P., Kath, W. L., & Motter, A. E. (2013) · Nature Communications · doi:10.1038/ncomms2939 · Cited by 340
Islam, M. A., Xu, Y., Tao, W., Ubellacker, J. M., Lim, M., Aum, D., Lee, G. Y., Zhou, K., Zope, H., Yu, M., et al. (2018) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0284-0 · Cited by 339
Mou, H., Zhao, R., Sherwood, R. I., Ahfeldt, T., Lapey, A., Wain, J. R., Sicilian, L., Izvolsky, K. I., Lau, F. H., Musunuru, K., et al. (2012) · Cell stem cell · doi:10.1016/j.stem.2012.01.018 · Cited by 316
Self-citationEfficient generation of human iPSCs by a synthetic self-replicative RNA
Yoshioka, N., Gros, E., Li, H.-R., Kumar, S., Deacon, D. C., Maron, C., Muotri, A. R., Chi, N. C., Fu, X.-D., Yu, B. D., et al. (2013) · Cell stem cell · doi:10.1016/j.stem.2013.06.001 · Cited by 312
Self-citationHajj, K. A., Ball, R. L., Deluty, S. B., Singh, S. R., Strelkova, D., Knapp, C. M., & Whitehead, K. A. (2019) · Small · doi:10.1002/smll.201805097 · Cited by 312
Sebastiano, V., Maeder, M. L., Angstman, J. F., Haddad, B., Khayter, C., Yeo, D. T., Goodwin, M. J., Hawkins, J. S., Ramirez, C. L., Batista, L. F. Z., et al. (2011) · Stem Cells · doi:10.1002/stem.718 · Cited by 311
Targeted gene knockout by direct delivery of zinc-finger nuclease proteins
Gaj, T., Guo, J., Kato, Y., Sirk, S. J., & Barbas, C. F. (2012) · Nature Methods · doi:10.1038/nmeth.2030 · Cited by 306
Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O’Bara, M. A., Sadiq, S., Sim, F. J., Goldman, J., & Fossati, V. (2014) · Stem Cell Reports · doi:10.1016/j.stemcr.2014.06.012 · Cited by 302
A double-edged sword of immuno-microenvironment in cardiac homeostasis and injury repair
Sun, K., Li, Y., & Jin, J. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00455-6 · Cited by 311
5-Methylcytosine RNA Methylation in Arabidopsis Thaliana
Cui, X., Liang, Z., Shen, L., Zhang, Q., Bao, S., Geng, Y., Zhang, B., Leo, V. I., Vardy, L. A., Lu, T., et al. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.09.013 · Cited by 299
Denning, C., Borgdorff, V., Crutchley, J., Firth, K. S. A., George, V., Kalra, S., Kondrashov, A., Hoang, M. D., Mosqueira, D., Patel, A., et al. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2015.10.014 · Cited by 293
“This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).”
BackgroundInfluentialProgress in understanding reprogramming to the induced pluripotent state
Plath, K., & Lowry, W. E. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2955 · Cited by 291
Turning straw into gold: directing cell fate for regenerative medicine
Cohen, D. E., & Melton, D. A. (2011) · Nature Reviews Genetics · doi:10.1038/nrg2938 · Cited by 299
Translation of Human-Induced Pluripotent Stem Cells
Sayed, N., Liu, C., & Wu, J. C. (2016) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2016.01.083 · Cited by 290
Genomic Instability of iPSCs: Challenges Towards Their Clinical Applications
Yoshihara, M., Hayashizaki, Y., & Murakawa, Y. (2017) · Stem Cell Reviews and Reports · doi:10.1007/s12015-016-9680-6 · Cited by 283
Itoh, M., Kiuru, M., Cairo, M. S., & Christiano, A. M. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100332108 · Cited by 282
Svitkin, Y. V., Cheng, Y. M., Chakraborty, T., Presnyak, V., John, M., & Sonenberg, N. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx135 · Cited by 278
Metadata
- Authors
- Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y.-H., Li, H., Lau, F., Ebina, W., Mandal, P. K., Smith, Z. D., Meissner, A., Daley, G. Q., Brack, A. S., Collins, J. J., Cowan, C., Schlaeger, T. M., & Rossi, D. J.
- Affiliations
- Boston Children's Hospital (US)
- Harvard University (US)
- Boston University (US)
- Broad Institute (US)
- Brigham and Women's Hospital (US)
- Corresponding author
- Rossi, Derrick J.
- ORCID
- Venue
- Cell Stem Cell
- Publisher
- Elsevier BV
- Published
- 2010-11
- Volume
- 7
- Issue
- 5
- Pages
- 618-630
- ISSN
- 1875-9777 (ISSN-L)
- 1934-5909
- PMC
- PMC3656821
- OpenAlex
- W2098597036
- Cited by
- 2,662
- References
- 55
- Citations by year
- 2026: 74 · 2025: 67 · 2024: 89 · 2023: 81 · 2022: 109 · 2021: 153 · 2020: 148 · 2019: 130 · 2018: 178 · 2017: 167 · 2016: 201 · 2015: 208 · 2014: 235 · 2013: 264 · 2012: 288
- FWCI
- 106.76
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 989 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell stem cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- Howard Hughes Medical Institute
- National Heart, Lung, and Blood Institute (U01HL100001)
- National Institute of Dental and Craniofacial Research (RL1DE01902, RL1DE019021)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- synthetic mRNA, somatic cell reprogramming, pluripotency, directed differentiation, myogenesis, antiviral response, disease modeling, regenerative medicine
- MeSH terms
- Cell Differentiation, Cells, Cultured, Humans, RNA, Messenger, Cell Lineage, Induced Pluripotent Stem Cells, Cellular Reprogramming
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
