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Cited by 1,601

The DOI matches its Crossref record.

Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow.

Open access
Hardy, R R, Carmack, C E, Shinton, S A, Kemp, J D, Hayakawa, K·1991·The Journal of experimental medicine
DOI 10.1084/jem.173.5.1213
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Abstract

We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others, expressing S7, include B lineage cells before pre-B. These S7+ fractions, provisionally termed Fr. A, Fr. B, and Fr. C, can differentiate in a stromal layer culture system. Phenotypic alteration during such culture suggests an ordering of these stages from Fr. A to Fr. B to Fr. C and thence to S7- pre-B cells. Using polymerase chain reaction amplification with pairs of oligonucleotide primers for regions 5' of JH1, DFL16.1, and Jk1, we find that the Ig genes of Fr. A are in germline configuration, whereas Fr. B and C are pro-B cell stages with increasing D-J rearrangement, but no V-D-J. Finally, functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr. A.

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Authors
Hardy, R. R., Carmack, C. E., Shinton, S. A., Kemp, J. D., & Hayakawa, K.
Venue
The Journal of experimental medicine
Publisher
Rockefeller University Press
Published
1991-05-01
Volume
173
Issue
5
Pages
1213-1225
ISSN
  • 0022-1007 (ISSN-L)
  • 1540-9538
DOI
10.1084/jem.173.5.1213
OpenAlex
W2069501059
Cited by
1,601
References
0
FWCI
21.96
Type
journal article
Language
en
Open access
green

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