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Cited by 4,722

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization

Open access
Spellman, Paul T., Sherlock, Gavin, Zhang, Michael Q., Iyer, Vishwanath R., Anders, Kirk, Eisen, Michael B., Brown, Patrick O., Botstein, David, Futcher, Bruce·1998·Molecular Biology of the Cell

Abstract

We sought to create a comprehensive catalog of yeast genes whose transcript levels vary periodically within the cell cycle. To this end, we used DNA microarrays and samples from yeast cultures synchronized by three independent methods: α factor arrest, elutriation, and arrest of a cdc15 temperature-sensitive mutant. Using periodicity and correlation algorithms, we identified 800 genes that meet an objective minimum criterion for cell cycle regulation. In separate experiments, designed to examine the effects of inducing either the G1 cyclin Cln3p or the B-type cyclin Clb2p, we found that the mRNA levels of more than half of these 800 genes respond to one or both of these cyclins. Furthermore, we analyzed our set of cell cycle–regulated genes for known and new promoter elements and show that several known elements (or variations thereof) contain information predictive of cell cycle regulation. A full description and complete data sets are available at http://cellcycle-www.stanford.edu

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Crossref · 10.1091/mbc.9.12.3273

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Metadata

Authors
Spellman, P. T., Sherlock, G., Zhang, M. Q., Iyer, V. R., Anders, K., Eisen, M. B., Brown, P. O., Botstein, D., & Futcher, B.
Venue
Molecular Biology of the Cell
Published
1998-12
Volume
9
Issue
12
Pages
3273-3297
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2103453943
Cited by
4,722
References
1
FWCI
49.64
Type
journal article
Language
en
Open access
green

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