Voluntary Running Exercise Alters Microbiota Composition and Increases n-Butyrate Concentration in the Rat Cecum
Abstract
The effects of voluntary wheel-running exercise on cecal microbiota and short-chain fatty acid production were investigated in rats. The microbiota composition was notably different between the exercised and sedentary rats. Furthermore, the exercised rats showed a significantly higher n-butyrate concentration than the sedentary rats. This alteration of the cecal microbial environment may contribute to the beneficial effect of exercise on gastrointestinal disorders.
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Regular running exercise in rats is related to an increase in butyrate-producing bacteria and an increase of butyrate concentration.
“Matsumoto et al. [72] reported that regular running exercise in rats was related to an increase in butyrate-producing bacteria in the microbiota composition along with an increase of butyrate concentration.”
From The athletic gut microbiota (2020) · cites this paper as [72]
The cecum was approximately 1.5 times larger in the exercise group than in the control group, and cecal tissue weights and contents were much greater in the exercise group, indicating a significant change in the cecal environment in response to voluntary wheel running.
“The cecum was approximately 1.5 times larger in the exercise group than in the control group, and the cecal tissue weights and contents were much greater in the exercise group than in the control group, indicating that a significant change had occurred in the cecal environment in response to voluntary wheel running [80].”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [80]
N-butyrate levels increased significantly in the exercise group (8.14 ± 1.36 mmol/g of cecal contents) compared to the control group (4.87 ± 0.41 mmol/g of cecal contents).
“Levels of N-butyrate increased significantly between the exercise groups (8.14 ± 1.36 mmol/g of cecal contents) compared to the control group (4.87 ± 0.41 mmol/g of cecal contents) [80].”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [80]
The cecal microbiota and SCFA profiles were much different between the exercise and control groups.
“Additionally, the cecal microbiota and SCFA profiles were much different between the exercise and control groups [80].”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [80]
In 14 male Wistar rats over five weeks, rats that voluntarily exercised using a wheel presented higher cecum levels of SCFA than the sedentary control group.
“Matsumoto et al [80] performed a study in 14 male Wistar rats during five weeks. The control group was sedentary and the exercise group had access to an exercise wheel in their cage. They discovered through 16S rRNA gene sequencing that the rats that voluntarily exercised using the wheel presented higher cecum levels of SCFA than the sedentary control group.”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [80]
Prolonged intense exercise could negatively affect neurotransmitter homeostasis and immune response when glutamine levels are depleted to detoxify ammonia in the brain, causing a more excitatory-glutamate driven response and a decline in GABA-mediated inhibitory pathways.
“Therefore, prolonged intense exercise could negatively affect neurotransmitter homeostasis and immune response when glutamine levels are depleted [80, 81] in order to detoxify ammonia in the brain causing a more excitatory-glutamate driven response to exercise-induced stress and a decline in GABA-mediated inhibitory pathways.”
From Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes (2016) · cites this paper as [80]
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- Authors
- MATSUMOTO, M., INOUE, R., TSUKAHARA, T., USHIDA, K., CHIJI, H., MATSUBARA, N., & HARA, H.
- Affiliations
- Hokkaido University (JP)
- Institute of Nutrition and Pathology (Japan) (JP)
- Kyoto Prefectural University (JP)
- Fuji Women's University (JP)
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-02-23
- Volume
- 72
- Issue
- 2
- Pages
- 572-576
- ISSN
- 0916-8451 (ISSN-L)
- 1347-6947
- OpenAlex
- W1975807766
- Cited by
- 352
- References
- 21
- Citations by year
- 2026: 17 · 2025: 26 · 2024: 37 · 2023: 35 · 2022: 43 · 2021: 37 · 2020: 24 · 2019: 27 · 2018: 25 · 2017: 24 · 2016: 21 · 2015: 11 · 2014: 8 · 2013: 3 · 2012: 3
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioscience Biotechnology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- voluntary wheel running, physical activity, gut microbiota, cecal microbiota, short-chain fatty acids, n-butyric acid, cecum, sedentary behavior, gut health
- MeSH terms
- Animals, Butyrates, Cecum, Male, Physical Conditioning, Animal, Rats, Wistar, Rats
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