A General Strategy for Development of Activatable NIR‐II Fluorescent Probes for In Vivo High‐Contrast Bioimaging
Abstract
Organic dye based NIR‐II fluorescent probes, owing to their high signal‐to‐background ratio and deeper penetration, are highly useful for deep‐tissue high‐contrast imaging in vivo. However, it is still a challenge to design activatable NIR‐II fluorescent probes. Here, a novel class of polymethine dyes (NIRII‐RTs), with bright (quantum yield up to 2.03 %), stable, and anti‐solvent quenching NIR‐II emission, together with large Stokes shifts, was designed. Significantly, the novel NIR‐II dyes NIRII‐RT3 and NIRII‐RT4, equipped with a carboxylic acid group, can serve as effective NIR‐II platforms for the design of activatable bioimaging probes with high contrast. As a proof of concept, a series of target‐activatable NIRII‐RT probes (NIRII‐RT‐pH, NIRII‐RT‐ATP and NIRII‐RT‐Hg) for pH, adenosine triphosphate (ATP), and metal‐ion detection, were synthesized. By applying the NIRII‐RT probe, the real‐time monitoring of drug‐induced hepatotoxicity was realized.
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Xanthene dyes, particularly rhodamines and fluoresceins, have attracted considerable attention in biomedical imaging owing to outstanding photophysical properties including high molar absorptivity, excellent photostability, facile functionalization, and tunable emission.
“Xanthene dyes, particularly rhodamines and fluoresceins, have attracted considerable attention in biomedical imaging owing to their outstanding photophysical properties, including high molar absorptivity, excellent photostability, facile functionalization, and tunable emission”
From Organic small-molecule NIR-II fluorophores for tumor phototheranostics (2026) · cites this paper as [171]
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- Authors
- Ren, T., Wang, Z., Xiang, Z., Lu, P., Lai, H., Yuan, L., Zhang, X., & Tan, W.
- Affiliations
- Hunan University (CN)
- Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 P. R. China
- Venue
- Angewandte Chemie International Edition
- Publisher
- Wiley
- Published
- 2020-11-03
- Volume
- 60
- Issue
- 2
- Pages
- 800-805
- ISSN
- 1433-7851 (ISSN-L)
- 1521-3773
- OpenAlex
- W3086680449
- Cited by
- 273
- References
- 49
- Citations by year
- 2026: 25 · 2025: 40 · 2024: 46 · 2023: 61 · 2022: 73 · 2021: 27 · 2020: 1
- FWCI
- 15.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Angewandte Chemie International Edition (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Key Technologies Research and Development Program (2019YFA0210100)
- National Natural Science Foundation of China (21890744, 21622504, 21877029)
- National Postdoctoral Program for Innovative Talents (BX20190110)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Keywords
- activatable probes, polymethine dyes, pH sensing, ATP detection, metal ion sensing, drug-induced liver injury, real-time monitoring, carboxyl groups
- MeSH terms
- Animals, Blood Vessels, Contrast Media, Fluorescent Dyes, Indocyanine Green, Lymph Nodes, Polymers, Spectroscopy, Near-Infrared, Mice, Chemical and Drug Induced Liver Injury, Optical Imaging
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