Principles of Fluorescence Spectroscopy

Electron-Hole Diffusion Lengths Exceeding 1 Micrometer in an Organometal Trihalide Perovskite Absorber

Stranks, S. D., Eperon, G. E., Grancini, G., Menelaou, C., Alcocer, M. J. P., Leijtens, T., Herz, L. M., Petrozza, A., & Snaith, H. J. (2013) · Science · doi:10.1126/science.1243982 · Cited by 10,413

Fluorescence based explosive detection: from mechanisms to sensory materials

Sun, X., Wang, Y., & Lei, Y. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00496a · Cited by 1,243

Small-Molecule Two-Photon Probes for Bioimaging Applications

Kim, H. M., & Cho, B. R. (2015) · Chemical Reviews · doi:10.1021/cr5004425 · Cited by 1,047

Exciton diffusion in organic semiconductors

Mikhnenko, O. V., Blom, P. W. M., & Nguyen, T.-Q. (2015) · Energy & Environmental Science · doi:10.1039/c5ee00925a · Cited by 997

Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared window

Welsher, K., Sherlock, S. P., & Dai, H. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1014501108 · Cited by 958

Fluorescence lifetime imaging – techniques and applications

BECKER, W. (2012) · Journal of Microscopy · doi:10.1111/j.1365-2818.2012.03618.x · Cited by 890

Ascorbic acid enhances Tet-mediated 5-methylcytosine oxidation and promotes DNA demethylation in mammals

Yin, R., Mao, S.-Q., Zhao, B., Chong, Z., Yang, Y., Zhao, C., Zhang, D., Huang, H., Gao, J., Li, Z., et al. (2013) · Journal of the American Chemical Society · doi:10.1021/ja4028346 · Cited by 609

Local, persistent activation of Rho GTPases during plasticity of single dendritic spines

Murakoshi, H., Wang, H., & Yasuda, R. (2011) · Nature · doi:10.1038/nature09823 · Cited by 564

Microenvironment mapping via Dexter energy transfer on immune cells

Geri, J. B., Oakley, J. V., Reyes-Robles, T., Wang, T., McCarver, S. J., White, C. H., Rodriguez-Rivera, F. P., Parker, D. L., Hett, E. C., Fadeyi, O. O., et al. (2020) · Science · doi:10.1126/science.aay4106 · Cited by 481

Molybdenum Disulfide Quantum Dots as a Photoluminescence Sensing Platform for 2,4,6-Trinitrophenol Detection

Wang, Y., & Ni, Y. (2014) · Analytical Chemistry · doi:10.1021/ac5012014 · Cited by 422

Orbital Occupancy and Spin Polarization: From Mechanistic Study to Rational Design of Transition Metal-Based Electrocatalysts toward Energy Applications

Do, V.-H., & Lee, J.-M. (2022) · ACS Nano · doi:10.1021/acsnano.2c08919 · Cited by 292

Structural Basis for Pattern Recognition by the Receptor for Advanced Glycation End Products (RAGE)

Xie, J., Reverdatto, S., Frolov, A., Hoffmann, R., Burz, D. S., & Shekhtman, A. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m801622200 · Cited by 252

Analysis of cholesterol trafficking with fluorescent probes

Maxfield, F. R., & Wüstner, D. (2012) · Methods in Cell Biology · doi:10.1016/b978-0-12-386487-1.00017-1 · Cited by 240

Plug-and-Play Optical Materials from Fluorescent Dyes and Macrocycles

Benson, C. R., Kacenauskaite, L., VanDenburgh, K. L., Zhao, W., Qiao, B., Sadhukhan, T., Pink, M., Chen, J., Borgi, S., Chen, C.-H., et al. (2020) · Chem · doi:10.1016/j.chempr.2020.06.029 · Cited by 226

Holographic Sensors: Three-Dimensional Analyte-Sensitive Nanostructures and Their Applications

Yetisen, A. K., Naydenova, I., da Cruz Vasconcellos, F., Blyth, J., & Lowe, C. R. (2014) · Chemical Reviews · doi:10.1021/cr500116a · Cited by 224

Near-Infrared Fluorescent Dye-Doped Semiconducting Polymer Dots

Jin, Y., Ye, F., Zeigler, M., Wu, C., & Chiu, D. T. (2011) · ACS Nano · doi:10.1021/nn103304m · Cited by 211

FRET Based Biosensor: Principle Applications Recent Advances and Challenges

Verma, A. K., Noumani, A., Yadav, A. K., & Solanki, P. R. (2023) · Diagnostics · doi:10.3390/diagnostics13081375 · Cited by 202

Oxygen control with microfluidics

Brennan, M. D., Rexius-Hall, M. L., Elgass, L. J., & Eddington, D. T. (2014) · Lab Chip · doi:10.1039/c4lc00853g · Cited by 188

Spectroscopic sensors for in-line bioprocess monitoring in research and pharmaceutical industrial application

Claßen, J., Aupert, F., Reardon, K. F., Solle, D., & Scheper, T. (2017) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-016-0068-x · Cited by 176

Giant light-harvesting nanoantenna for single-molecule detection in ambient light

Trofymchuk, K., Reisch, A., Didier, P., Fras, F., Gilliot, P., Mely, Y., & Klymchenko, A. S. (2017) · Nature Photonics · doi:10.1038/s41566-017-0001-7 · Cited by 175

Fluorescence lifetime imaging microscopy in life sciences

Borst, J. W., & Visser, A. J. W. G. (2010) · Measurement Science and Technology · doi:10.1088/0957-0233/21/10/102002 · Cited by 168

Postsynaptic signaling during plasticity of dendritic spines

Murakoshi, H., & Yasuda, R. (2012) · Trends in Neurosciences · doi:10.1016/j.tins.2011.12.002 · Cited by 158

Aggregation-induced emission (AIE)-active polymers for explosive detection

Zhou, H., Chua, M. H., Tang, B. Z., & Xu, J. (2019) · Polymer Chemistry · doi:10.1039/c9py00322c · Cited by 156

Apocynin: Chemical and Biophysical Properties of a NADPH Oxidase Inhibitor

Petrônio, M., Zeraik, M., Fonseca, L., & Ximenes, V. (2013) · Molecules · doi:10.3390/molecules18032821 · Cited by 149

Two-photon excitation STED microscopy in two colors in acute brain slices

Bethge, P., Chéreau, R., Avignone, E., Marsicano, G., & Nägerl, U. V. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2012.12.054 · Cited by 139

AIE Polymers: Synthesis, Properties, and Biological Applications

Zhan, R., Pan, Y., Manghnani, P. N., & Liu, B. (2017) · Macromolecular Bioscience · doi:10.1002/mabi.201600433 · Cited by 132

Pepsin diffusivity in whey protein gels and its effect on gastric digestion

Luo, Q., Borst, J. W., Westphal, A. H., Boom, R. M., & Janssen, A. E. M. (2017) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2016.11.046 · Cited by 123

On mechanisms of fluorescence quenching by water

Dobretsov, G. E., Syrejschikova, T. I., & Smolina, N. V. (2014) · Biophysics · doi:10.1134/s0006350914020079 · Cited by 123

Fluorescence Spectroscopy and Chemometric Modeling for Bioprocess Monitoring

Faassen, S., & Hitzmann, B. (2015) · Sensors · doi:10.3390/s150510271 · Cited by 120

Targeting Protein–Protein Interactions and Fragment-Based Drug Discovery

Valkov, E., Sharpe, T., Marsh, M., Greive, S., & Hyvönen, M. (2011) · Topics in Current Chemistry · doi:10.1007/128_2011_265 · Cited by 107

Two-way communication between SecY and SecA suggests a Brownian ratchet mechanism for protein translocation.

Allen, W. J., Corey, R. A., Oatley, P., Sessions, R. B., Baldwin, S. A., Radford, S. E., Tuma, R., & Collinson, I. (2016) · eLife · doi:10.7554/elife.15598 · Cited by 103

Cytosolic NADH-NAD + Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging

Mongeon, R., Venkatachalam, V., & Yellen, G. (2016) · Antioxidants & Redox Signaling · doi:10.1089/ars.2015.6593 · Cited by 100

The fluorescence properties of aerosol larger than 0.8 μm in urban and tropical rainforest locations

Gabey, A. M., Stanley, W. R., Gallagher, M. W., & Kaye, P. H. (2011) · Atmospheric Chemistry and Physics · doi:10.5194/acp-11-5491-2011 · Cited by 97

Ultraviolet superradiance from mega-networks of tryptophan in biological architectures

Babcock, N. S., Montes-Cabrera, G., Oberhofer, K. E., Chergui, M., Celardo, G. L., & Kurian, P. (2024) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.3c07936 · Cited by 93

Bandwidth limits of luminescent solar concentrators as detectors in free-space optical communication systems

Portnoi, M., Haigh, P. A., Macdonald, T. J., Ambroz, F., Parkin, I. P., Darwazeh, I., & Papakonstantinou, I. (2021) · Light: Science & Applications · doi:10.1038/s41377-020-00444-y · Cited by 93

Critical new insights into the binding of poly- and perfluoroalkyl substances (PFAS) to albumin protein

Alesio, J. L., Slitt, A., & Bothun, G. D. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2021.131979 · Cited by 91

Spin-Polarized Photon Emission by Resonant Multipolar Nanoantennas

Kruk, S. S., Decker, M., Staude, I., Schlecht, S., Greppmair, M., Neshev, D. N., & Kivshar, Y. S. (2014) · ACS Photonics · doi:10.1021/ph500288u · Cited by 83

Toward Prediction of Nonradiative Decay Pathways in Organic Compounds II: Two Internal Conversion Channels in BODIPYs

Lin, Z., Kohn, A. W., & Van Voorhis, T. (2020) · The Journal of Physical Chemistry C · doi:10.1021/acs.jpcc.9b08292 · Cited by 78

Resazurin Reduction-Based Assays Revisited: Guidelines for Accurate Reporting of Relative Differences on Metabolic Status

Vieira-da-Silva, B., & Castanho, M. A. R. B. (2023) · Molecules · doi:10.3390/molecules28052283 · Cited by 76

Origin of the Red-Shifted Optical Spectra Recorded for Aza-BODIPY Dyes

Karlsson, J. K. G., & Harriman, A. (2016) · The Journal of Physical Chemistry A · doi:10.1021/acs.jpca.6b01278 · Cited by 68

Molecular weight dependence of emission intensity and emitting sites distribution within single conjugated polymer molecules

Habuchi, S., Onda, S., & Vacha, M. (2011) · Phys. Chem. Chem. Phys. · doi:10.1039/c0cp01729a · Cited by 65

Observations of fluorescent and biological aerosol at a high-altitude site in central France

Gabey, A. M., Vaitilingom, M., Freney, E., Boulon, J., Sellegri, K., Gallagher, M. W., Crawford, I. P., Robinson, N. H., Stanley, W. R., & Kaye, P. H. (2013) · Atmospheric Chemistry and Physics · doi:10.5194/acp-13-7415-2013 · Cited by 64

Lrat-specific domain facilitates Vitamin A metabolism by domain swapping in HRASLS3

Golczak, M., Sears, A. E., Kiser, P. D., & Palczewski, K. (2015) · Nature Chemical Biology · doi:10.1038/nchembio.1687 · Cited by 58

A novel lysosome‐to‐mitochondria signaling pathway disrupted by amyloid‐β oligomers

Norambuena, A., Wallrabe, H., Cao, R., Wang, D. B., Silva, A., Svindrych, Z., Periasamy, A., Hu, S., Tanzi, R. E., Kim, D. Y., et al. (2018) · The EMBO Journal · doi:10.15252/embj.2018100241 · Cited by 61

Measuring and regulating oxygen levels in microphysiological systems: design, material, and sensor considerations

Rivera, K. R., Yokus, M. A., Erb, P. D., Pozdin, V. A., & Daniele, M. (2019) · The Analyst · doi:10.1039/c8an02201a · Cited by 57

sAβPPα is a Potent Endogenous Inhibitor of BACE1

Peters-Libeu, C., Campagna, J., Mitsumori, M., Poksay, K. S., Spilman, P., Sabogal, A., Bredesen, D. E., & John, V. (2015) · Journal of Alzheimer's Disease · doi:10.3233/jad-150282 · Cited by 58

Aggregation induced enhanced emission of conjugated dendrimers with a large intrinsic two-photon absorption cross-section

Xu, B., Zhang, J., Fang, H., Ma, S., Chen, Q., Sun, H., Im, C., & Tian, W. (2014) · Polym. Chem. · doi:10.1039/c3py00974b · Cited by 56

Conjugated Polymer-Based Hybrid Nanoparticles with Two-Photon Excitation and Near-Infrared Emission Features for Fluorescence Bioimaging within the Biological Window

Lv, Y., Liu, P., Ding, H., Wu, Y., Yan, Y., Liu, H., Wang, X., Huang, F., Zhao, Y., & Tian, Z. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b05150 · Cited by 56

Fluorescence Lifetime Imaging Microscopy for Quantitative Biological Imaging

Chen, L.-C., Lloyd, W. R., Chang, C.-W., Sud, D., & Mycek, M.-A. (2013) · Methods in Cell Biology · doi:10.1016/b978-0-12-407761-4.00020-8 · Cited by 55

What limits photosynthetic energy conversion efficiency in nature? Lessons from the oceans

Falkowski, P. G., Lin, H., & Gorbunov, M. Y. (2017) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2016.0376 · Cited by 52

Efficient ultrafast laser writing with elliptical polarization

Lei, Y., Shayeganrad, G., Wang, H., Sakakura, M., Yu, Y., Wang, L., Kliukin, D., Skuja, L., Svirko, Y., & Kazansky, P. G. (2023) · Light: Science & Applications · doi:10.1038/s41377-023-01098-2 · Cited by 48

Apolipoprotein E4 has extensive conformational heterogeneity in lipid-free and lipid-bound forms

Stuchell-Brereton, M. D., Zimmerman, M. I., Miller, J. J., Mallimadugula, U. L., Incicco, J. J., Roy, D., Smith, L. G., Cubuk, J., Baban, B., DeKoster, G. T., et al. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2215371120 · Cited by 48

Comprehensive Experimental and Computational Analysis of Binding Energy Hot Spots at the NF-κB Essential Modulator/IKKβ Protein–Protein Interface

Golden, M. S., Cote, S. M., Sayeg, M., Zerbe, B. S., Villar, E. A., Beglov, D., Sazinsky, S. L., Georgiadis, R. M., Vajda, S., Kozakov, D., et al. (2013) · Journal of the American Chemical Society · doi:10.1021/ja400914z · Cited by 47

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao, F., Sá, M., Teles (Cabanelas, I., Wijffels, R. H., & Barbosa, M. J. (2021) · Biotechnology and Bioengineering · doi:10.1002/bit.27657 · Cited by 47

Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells

Takahashi, N., Sawada, W., Noguchi, J., Watanabe, S., Ucar, H., Hayashi-Takagi, A., Yagishita, S., Ohno, M., Tokumaru, H., & Kasai, H. (2015) · Nature Communications · doi:10.1038/ncomms9531 · Cited by 46

The Na(+)/K(+) pump dominates control of glycolysis in hippocampal dentate granule cells

Meyer, D. J., Díaz-García, C. M., Nathwani, N., Rahman, M., & Yellen, G. (2022) · eLife · doi:10.7554/elife.81645 · Cited by 40

Near‐Infrared Fluorophores Based on Heptamethine Cyanine Dyes: From Their Synthesis and Photophysical Properties to Recent Optical Sensing and Bioimaging Applications

Medeiros, N. G., Braga, C. A., Câmara, V. S., Duarte, R. C., & Rodembusch, F. S. (2022) · Asian Journal of Organic Chemistry · doi:10.1002/ajoc.202200095 · Cited by 36

Fluorescence spectroscopy of atmospherically relevant bacterial and fungal spores and potential interferences

Saari, S. E., Putkiranta, M. J., & Keskinen, J. (2013) · Atmospheric Environment · doi:10.1016/j.atmosenv.2013.02.023 · Cited by 35

Fatty Acids Bind Tightly to the N-terminal Domain of Angiopoietin-like Protein 4 and Modulate Its Interaction with Lipoprotein Lipase

Robal, T., Larsson, M., Martin, M., Olivecrona, G., & Lookene, A. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.303529 · Cited by 30

Perspectives of fluorescence spectroscopy for online monitoring in microalgae industry

Sá, M., Ferrer‐Ledo, N., Gao, F., Bertinetto, C. G., Jansen, J., Crespo, J. G., Wijffels, R. H., Barbosa, M., & Galinha, C. F. (2022) · Microbial Biotechnology · doi:10.1111/1751-7915.14013 · Cited by 29

Cell culture media analysis using rapid spectroscopic methods

Ryder, A. G. (2018) · Current Opinion in Chemical Engineering · doi:10.1016/j.coche.2018.08.008 · Cited by 30

What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding

Farrens, D. L. (2010) · Photochemical & Photobiological Sciences · doi:10.1039/c0pp00283f · Cited by 28

Strong difference between optical properties and morphologies for J-Aggregates of similar cyanine dyes

Sorokin, A. V., Ropakova, I. Y., Grynyov, R. S., Vilkisky, M. M., Liakh, V. M., Borovoy, I. A., Yefimova, S. L., & Malyukin, Y. V. (2018) · Dyes and Pigments · doi:10.1016/j.dyepig.2018.01.032 · Cited by 27

Sensitized ZnO nanorod assemblies to detect heavy metal contaminated phytomedicines: Spectroscopic and simulation studies

Bagchi, D., Maji, T. K., Sardar, S., Lemmens, P., Bhattacharya, C., Karmakar, D., & Pal, S. K. (2017) · Physical Chemistry Chemical Physics · doi:10.1039/c6cp08016b · Cited by 25

Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life

Jensen, G. C., Janis, M. K., Nguyen, H. N., David, O. W., & Zastrow, M. L. (2024) · ACS Sensors · doi:10.1021/acssensors.3c02695 · Cited by 25

Förster Resonance Energy Transfer Methods for Quantification of Protein–Protein Interactions on Microarrays

Schäferling, M., & Nagl, S. (2011) · Methods in Molecular Biology · doi:10.1007/978-1-61779-043-0_19 · Cited by 22

Next-Generation Wearable Optical Sensors for Personalized Health and Point-of-Care Diagnostics—A Systematic Review

Duarah, R., Torné‐Morató, H., Zhang, G., Amin, Y., Pabast, M., Sharma, N., He, S., Das, M. R., & Pompa, P. P. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.202504419 · Cited by 24

Basics of Optical Spectroscopy

Hof, M., & Macháň, R. (2014) · Handbook of Spectroscopy · doi:10.1002/9783527654703.ch3 · Cited by 18

Methylene Blue Blocks and Reverses the Inhibitory Effect of Tau on PMCA Function

Berrocal, M., Caballero-Bermejo, M., Gutierrez-Merino, C., & Mata, A. M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20143521 · Cited by 17

Understanding the good and poor cell targeting activity of gold nanostructures functionalized with molecular units for the epidermal growth factor receptor

Mazzuca, C., Di Napoli, B., Biscaglia, F., Ripani, G., Rajendran, S., Braga, A., Benna, C., Mocellin, S., Gobbo, M., Meneghetti, M., et al. (2019) · Nanoscale Advances · doi:10.1039/c9na00096h · Cited by 16

MAP2 caps tau fibrils and inhibits aggregation

Holden, M. R., Krzesinski, B. J., Weismiller, H. A., Shady, J. R., & Margittai, M. (2023) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2023.104891 · Cited by 13

Apolipoprotein E4 exhibits intermediates with domain interaction

Dolai, S., Cherakara, S., & Garai, K. (2020) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2020.140535 · Cited by 13

Structural insights into light harvesting by antenna-containing rhodopsins in marine Asgard archaea

Tzlil, G., Marín, M. D. C., Matsuzaki, Y., Nag, P., Itakura, S., Mizuno, Y., Murakoshi, S., Tanaka, T., Larom, S., Konno, M., et al. (2025) · Nature Microbiology · doi:10.1038/s41564-025-02016-5 · Cited by 9

Implementation of Innovative Process Analytical Technologies to Characterize Critical Quality Attributes of Co‐Formulated Monoclonal Antibody Products

Godbole, A., Chen, L., Desai, J., Raghava, S., Ruzanski, R., Patel, B., Appiah‐Amponsah, E., & Feng, H. (2025) · Biotechnology and Bioengineering · doi:10.1002/bit.28881 · Cited by 4

Flavoproteins as native and genetically encoded spin probes for in cell ESR spectroscopy

(2025) · doi:10.1038/s41467-025-60623-6 · Cited by 4

Interrogating the structure and function of the human voltage-gated proton channel (hHv1) with a fluorescent noncanonical amino acid

Carmona, E. M., Zagotta, W. N., & Gordon, S. E. (2025) · doi:10.1101/2025.10.23.684235

Interrogating the structure and function of the human voltage-gated proton channel (hHv1) with a fluorescent noncanonical amino acid

Carmona, E. M., Zagotta, W. N., & Gordon, S. E. (2026) · eLife · doi:10.7554/elife.110161