Homeostasis in intestinal epithelium is orchestrated by the circadian clock and microbiota cues transduced by TLRs
Open accessAbstract
Alterations of symbiosis between microbiota and intestinal epithelial cells (IEC) are associated with intestinal and systemic pathologies. Interactions between bacterial products (MAMPs) and Toll-like receptors (TLRs) are known to be mandatory for IEC homeostasis, but how TLRs may time homeostatic functions with circadian changes is unknown. Our functional and molecular dissections of the IEC circadian clock demonstrate that its integrity is required for microbiota-IEC dialog. In IEC, the antiphasic expression of the RORα activator and RevErbα repressor clock output regulators generates a circadian rhythmic TLR expression that converts the temporally arrhythmic microbiota signaling into circadian rhythmic JNK and IKKβ activities, which prevents RevErbα activation by PPARα that would disrupt the circadian clock. Moreover, through activation of AP1 and NF-κB, these activities, together with RORα and RevErbα, enable timing homeostatic functions of numerous genes with IEC circadian events. Interestingly, microbiota signaling deficiencies induce a prediabetic syndrome due to ileal corticosterone overproduction consequent to clock disruption.
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There is only preliminary evidence that under basal conditions the GM contributes to the regulation of intestinal glucocorticoid production.
“However, there is only preliminary evidence available demonstrating that under basal conditions the GM contributes to the regulation of intestinal glucocorticoid production (527, 528).”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [528]
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- Authors
- Mukherji, A., Kobiita, A., Ye, T., & Chambon, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Chambon, Pierre
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 153
- Issue
- 4
- Pages
- 812-827
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2051984731
- Cited by
- 560
- References
- 61
- Citations by year
- 2026: 21 · 2025: 36 · 2024: 45 · 2023: 43 · 2022: 58 · 2021: 43 · 2020: 54 · 2019: 68 · 2018: 24 · 2017: 48 · 2016: 39 · 2015: 39 · 2014: 37 · 2013: 5
- FWCI
- 21.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche (07-Physio 002-01)
- Institut National de la Santé et de la Recherche Médicale
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- intestinal epithelium, circadian clock, gut microbiota, Toll-like receptors, RORα, NR1D1, PPARα, JNK, IKKβ, NF-κB, AP-1, prediabetes, corticosterone, intestinal homeostasis
- MeSH terms
- Animals, Corticosterone, Ileum, Intestinal Mucosa, Male, Mice, Inbred C57BL, NF-kappa B, Toll-Like Receptors, Mice, Metagenome, Nuclear Receptor Subfamily 1, Group D, Member 1, Nuclear Receptor Subfamily 1, Group F, Member 1
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