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Porous 3-D Scaffolds from Regenerated Silk Fibroin
Abstract
Three fabrication techniques, freeze-drying, salt leaching and gas foaming, were used to form porous three-dimensional silk biomaterial matrixes. Matrixes were characterized for morphological and functional properties related to processing method and conditions. The porosity of the salt leached scaffolds varied between 84 and 98% with a compressive strength up to 175 +/- 3 KPa, and the gas foamed scaffolds had porosities of 87-97% and compressive strength up to 280 +/- 4 KPa. The freeze-dried scaffolds were prepared at different freezing temperatures (-80 and -20 degrees C) and subsequently treated with different concentrations (15 and 25%) and hydrophilicity alcohols. The porosity of these scaffolds was up to 99%, and the maximum compressive strength was 30 +/- 2 KPa. Changes in silk fibroin structure during processing to form the 3D matrixes were determined by FT-IR and XrD. The salt leached and gas foaming techniques produced scaffolds with a useful combination of high compressive strength, interconnected pores, and pore sizes greater than 100 microns in diameter. The results suggest that silk-based 3D matrixes can be formed for utility in biomaterial applications.
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Metadata
- Authors
- Nazarov, R., Jin, H.-J., & Kaplan, D. L.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-01-23
- Volume
- 5
- Issue
- 3
- Pages
- 718-726
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2091107068
- Cited by
- 888
- References
- 19
- FWCI
- 13.36
- Type
- journal article
- Language
- en
- Open access
- closed
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