Cited by 1,111Sleep and Wakefulness ResearchNeuroscience of respiration and sleepCircadian rhythm and melatonin

Activation of ventrolateral preoptic neurons during sleep

Sherin, J. E., Shiromani, P. J., McCarley, R. W., Saper, C. B.·1996·Science

Abstract

The rostral hypothalamus and adjacent basal forebrain participate in the generation of sleep, but the neuronal circuitry involved in this process remains poorly characterized. Immunocytochemistry was used to identify the FOS protein, an immediate-early gene product, in a group of ventrolateral preoptic neurons that is specifically activated during sleep. The retrograde tracer cholera toxin B, in combination with FOS immunocytochemistry, was used to show that sleep-activated ventrolateral preoptic neurons innervate the tuberomammillary nucleus, a posterior hypothalamic cell group thought to participate in the modulation of arousal. This monosynaptic pathway in the hypothalamus may play a key role in determining sleep-wake states.

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Crossref · 10.1126/science.271.5246.216

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46 of 46 references verified0 citation errors · 0 not checkable

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Authors
Sherin, J. E., Shiromani, P. J., McCarley, R. W., & Saper, C. B.
Affiliations
Harvard University (US)
Corresponding author
Saper, C. B.
Venue
Science
Published
1996-01-12
Volume
271
Issue
5246
Pages
216-219
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1989776859
Cited by
1,111
References
46
Citations by year
2026: 21 · 2025: 24 · 2024: 25 · 2023: 33 · 2022: 30 · 2021: 43 · 2020: 24 · 2019: 48 · 2018: 54 · 2017: 46 · 2016: 42 · 2015: 42 · 2014: 31 · 2013: 43 · 2012: 53
FWCI
16.77
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ns022835-11, 1f31mh010709-01)
  • National Institute of Mental Health (MH10709)
  • National Institute of Neurological Disorders and Stroke (NS30140, NS-22835, R01NS030140)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Neuroscience of respiration and sleep
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
Keywords
ventrolateral preoptic nucleus, sleep, FOS, immediate early genes, tuberomammillary nucleus, hypothalamus, basal forebrain, sleep-wake regulation, arousal, monosynaptic pathway, neuronal activation
MeSH terms
Animals, Brain Chemistry, Cholera Toxin, Circadian Rhythm, Immunohistochemistry, Mammillary Bodies, Neural Pathways, Neurons, Preoptic Area, Sleep, Proto-Oncogene Proteins c-fos, Rats

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