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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
Open accessAbstract
Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary to short sequence elements within the 16S rRNA common to phylogenetically coherent assemblages of microorganisms, were labeled with tetramethylrhodamine and hybridized to suspensions of fixed cells. Flow cytometry was used to resolve individual target and nontarget bacteria (1 to 5 microns) via probe-conferred fluorescence. Target cells were quantified in an excess of nontarget cells. The intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.
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Durand, L., Zbinden, M., Cueff-Gauchard, V., Duperron, S., Roussel, E. G., Shillito, B., & Cambon-Bonavita, M.-A. (2010) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2009.00806.x · Cited by 100
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Jan, C., Petersen, J. M., Werner, J., Teeling, H., Huang, S., Glöckner, F. O., Golyshina, O. V., Dubilier, N., Golyshin, P. N., Jebbar, M., et al. (2014) · Environmental Microbiology · doi:10.1111/1462-2920.12406 · Cited by 83
Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.-P., Rothballer, M., Schmid, M., & Bashan, Y. (2012) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2011.08.007 · Cited by 82
de-Bashan, L. E., Mayali, X., Bebout, B. M., Weber, P. K., Detweiler, A. M., Hernandez, J. P., Prufert-Bebout, L., & Bashan, Y. (2016) · Algal Research · doi:10.1016/j.algal.2016.02.019 · Cited by 79
Hügler, M., Petersen, J. M., Dubilier, N., Imhoff, J. F., & Sievert, S. M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016018 · Cited by 80
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Yang, Y., Shi, Y., Wiklund, P., Tan, X., Wu, N., Zhang, X., Tikkanen, O., Zhang, C., Munukka, E., & Cheng, S. (2017) · Nutrients · doi:10.3390/nu9080792 · Cited by 72
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Laso-Pérez, R., Wu, F., Crémière, A., Speth, D. R., Magyar, J. S., Zhao, K., Krupovic, M., & Orphan, V. J. (2023) · Nature Microbiology · doi:10.1038/s41564-022-01297-4 · Cited by 68
Stokke, R., Dahle, H., Roalkvam, I., Wissuwa, J., Daae, F. L., Tooming‐Klunderud, A., Thorseth, I. H., Pedersen, R. B., & Steen, I. H. (2015) · Environmental Microbiology · doi:10.1111/1462-2920.12970 · Cited by 63
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Metadata
- Authors
- Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., & Stahl, D. A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1990-06
- Volume
- 56
- Issue
- 6
- Pages
- 1919-1925
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1701869895
- Cited by
- 4,021
- References
- 0
- FWCI
- 43.87
- Type
- journal article
- Language
- en
- Open access
- bronze
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
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