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Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations

Open access
Amann, R I, Binder, B J, Olson, R J, Chisholm, S W, Devereux, R, Stahl, D A·1990·Applied and Environmental Microbiology

Abstract

Fluorescent oligonucleotide hybridization probes were used to label bacterial cells for analysis by flow cytometry. The probes, complementary to short sequence elements within the 16S rRNA common to phylogenetically coherent assemblages of microorganisms, were labeled with tetramethylrhodamine and hybridized to suspensions of fixed cells. Flow cytometry was used to resolve individual target and nontarget bacteria (1 to 5 microns) via probe-conferred fluorescence. Target cells were quantified in an excess of nontarget cells. The intensity of fluorescence was increased additively by the combined use of two or three fluorescent probes complementary to different regions of the same 16S rRNA.

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Crossref · 10.1128/aem.56.6.1919-1925.1990

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Metadata

Authors
Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R., & Stahl, D. A.
Venue
Applied and Environmental Microbiology
Published
1990-06
Volume
56
Issue
6
Pages
1919-1925
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W1701869895
Cited by
4,021
References
0
FWCI
43.87
Type
journal article
Language
en
Open access
bronze
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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