The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells
Abstract
Exosomes are nanovesicles released by leukocytes and epithelial cells. Although their function remains enigmatic, exosomes are a source of antigen and transfer functional major histocompatibility complex (MHC)–I/peptide complexes to dendritic cells (DCs) for CD8+ T-cell activation. Here we demonstrate that exosomes also are internalized and processed by immature DCs for presentation to CD4+ T cells. Endocytosed exosomes are sorted into the endocytic compartment of DCs for processing, followed by loading of exosome-derived peptides in MHC-II molecules for presentation to CD4+ T cells. Targeting of exosomes to DCs is mediated via milk fat globule (MFG)–E8/lactadherin, CD11a, CD54, phosphatidylserine, and the tetraspanins CD9 and CD81 on the exosome and αv/β3 integrin, and CD11a and CD54 on the DCs. Circulating exosomes are internalized by DCs and specialized phagocytes of the spleen and by hepatic Kupffer cells. Internalization of blood-borne allogeneic exosomes by splenic DCs does not affect DC maturation and is followed by loading of the exosome-derived allopeptide IEα52-68 in IAb by host CD8α+ DCs for presentation to CD4+ T cells. These data imply that exosomes present in circulation or extracellular fluids constitute an alternative source of self- or allopeptides for DCs during maintenance of peripheral tolerance or initiation of the indirect pathway of allorecognition in transplantation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
B lymphocytes secrete antigen-presenting vesicles.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546
Identification of a factor that links apoptotic cells to phagocytes
Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., & Nagata, S. (2002) · Nature · doi:10.1038/417182a · Cited by 1,401
Cited by
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834
Shedding light on the cell biology of extracellular vesicles
van Niel, G., D'Angelo, G., & Raposo, G. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.125 · Cited by 8,369
Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Biological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Routes and mechanisms of extracellular vesicle uptake
Mulcahy, L. A., Pink, R. C., & Carter, D. R. F. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24641 · Cited by 2,832
Regulation of immune responses by extracellular vesicles
Robbins, P. D., & Morelli, A. E. (2014) · Nature Reviews Immunology · doi:10.1038/nri3622 · Cited by 2,260
Self-citationMechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes.
Montecalvo, A., Larregina, A. T., Shufesky, W. J., Beer Stolz, D., Sullivan, M. L. G., Karlsson, J. M., Baty, C. J., Gibson, G. A., Erdos, G., Wang, Z., et al. (2012) · Blood · doi:10.1182/blood-2011-02-338004 · Cited by 1,429
Self-citationAlzheimer’s disease β-amyloid peptides are released in association with exosomes
Rajendran, L., Honsho, M., Zahn, T. R., Keller, P., Geiger, K. D., Verkade, P., & Simons, K. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0603838103 · Cited by 1,343
Ha, D., Yang, N., & Nadithe, V. (2016) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2016.02.001 · Cited by 1,346
Challenges and directions in studying cell–cell communication by extracellular vesicles
van Niel, G., Carter, D. R. F., Clayton, A., Lambert, D. W., Raposo, G., & Vader, P. (2022) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00460-3 · Cited by 1,229
Human saliva, plasma and breast milk exosomes contain RNA: Uptake by macrophages
Lässer, C., Seyed Alikhani, V., Ekström, K., Eldh, M., Torregrosa Paredes, P., Bossios, A., Sjöstrand, M., Gabrielsson, S., Lötvall, J., & Valadi, H. (2011) · Journal of Translational Medicine · doi:10.1186/1479-5876-9-9 · Cited by 899
Exosomes are released by cultured cortical neurones
Fauré, J., Lachenal, G., Court, M., Hirrlinger, J., Chatellard-Causse, C., Blot, B., Grange, J., Schoehn, G., Goldberg, Y., Boyer, V., et al. (2006) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2005.12.003 · Cited by 896
Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis
Fitzner, D., Schnaars, M., van Rossum, D., Krishnamoorthy, G., Dibaj, P., Bakhti, M., Regen, T., Hanisch, U.-K., & Simons, M. (2011) · Journal of Cell Science · doi:10.1242/jcs.074088 · Cited by 866
Acid sphingomyelinase activity triggers microparticle release from glial cells
Bianco, F., Perrotta, C., Novellino, L., Francolini, M., Riganti, L., Menna, E., Saglietti, L., Schuchman, E. H., Furlan, R., Clementi, E., et al. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.45 · Cited by 615
Endogenous RNAs modulate microRNA sorting to exosomes and transfer to acceptor cells.
Squadrito, M. L., Baer, C., Burdet, F., Maderna, C., Gilfillan, G. D., Lyle, R., Ibberson, M., & De Palma, M. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.07.035 · Cited by 604
Lai, C. P., Kim, E. Y., Badr, C. E., Weissleder, R., Mempel, T. R., Tannous, B. A., & Breakefield, X. O. (2015) · Nature Communications · doi:10.1038/ncomms8029 · Cited by 574
Qin, Y., Wang, L., Gao, Z., Chen, G., & Zhang, C. (2016) · Scientific Reports · doi:10.1038/srep21961 · Cited by 458
Extracellular vesicles in neurodegenerative disease — pathogenesis to biomarkers
Thompson, A. G., Gray, E., Heman-Ackah, S. M., Mäger, I., Talbot, K., Andaloussi, S. E., Wood, M. J., & Turner, M. R. (2016) · Nature Reviews Neurology · doi:10.1038/nrneurol.2016.68 · Cited by 431
The ins-and-outs of exosome biogenesis, secretion, and internalization
Arya, S. B., Collie, S. P., & Parent, C. A. (2024) · Trends in Cell Biology · doi:10.1016/j.tcb.2023.06.006 · Cited by 427
Cell-cell communication: New insights and clinical implications
Su, J., Song, Y., Zhu, Z., Huang, X., Fan, J., Qiao, J., & Mao, F. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01888-z · Cited by 433
Exosomes: Vehicles for the Transfer of Toxic Proteins Associated with Neurodegenerative Diseases?
Bellingham, S. A., Guo, B. B., Coleman, B. M., & Hill, A. F. (2012) · Frontiers in Physiology · doi:10.3389/fphys.2012.00124 · Cited by 412
Microglia: Agents of the CNS Pro-Inflammatory Response
Rodríguez-Gómez, J. A., Kavanagh, E., Engskog-Vlachos, P., Engskog, M. K. R., Herrera, A. J., Espinosa-Oliva, A. M., Joseph, B., Hajji, N., Venero, J. L., & Burguillos, M. A. (2020) · Cells · doi:10.3390/cells9071717 · Cited by 365
Exosome biogenesis, bioactivities and functions as new delivery systems of natural compounds
Farooqi, A. A., Desai, N. N., Qureshi, M. Z., Librelotto, D. R. N., Gasparri, M. L., Bishayee, A., Nabavi, S. M., Curti, V., & Daglia, M. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.12.010 · Cited by 359
Lipid-based carriers of microRNAs and intercellular communication
Vickers, K. C., & Remaley, A. T. (2012) · Current Opinion in Lipidology · doi:10.1097/mol.0b013e328350a425 · Cited by 314
Plant derived edible nanoparticles as a new therapeutic approach against diseases
Zhang, M., Viennois, E., Xu, C., & Merlin, D. (2016) · Tissue Barriers · doi:10.1080/21688370.2015.1134415 · Cited by 311
Iraci, N., Leonardi, T., Gessler, F., Vega, B., & Pluchino, S. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020171 · Cited by 301
Pregnancy-Associated Exosomes and Their Modulation of T Cell Signaling
Taylor, D. D., Akyol, S., & Gercel-Taylor, C. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.3.1534 · Cited by 297
RetractedInternalization of Exosomes through Receptor-Mediated Endocytosis
Gonda, A., Kabagwira, J., Senthil, G. N., & Wall, N. R. (2019) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-18-0891 · Cited by 296
The function and clinical application of extracellular vesicles in innate immune regulation
Zhou, X., Xie, F., Wang, L., Zhang, L., Zhang, S., Fang, M., & Zhou, F. (2020) · Cellular & Molecular Immunology · doi:10.1038/s41423-020-0391-1 · Cited by 294
Alzheimer’s disease brain-derived extracellular vesicles spread tau pathology in interneurons
Ruan, Z., Pathak, D., Venkatesan Kalavai, S., Yoshii-Kitahara, A., Muraoka, S., Bhatt, N., Takamatsu-Yukawa, K., Hu, J., Wang, Y., Hersh, S., et al. (2021) · Brain · doi:10.1093/brain/awaa376 · Cited by 283
Coakley, G., McCaskill, J. L., Borger, J. G., Simbari, F., Robertson, E., Millar, M., Harcus, Y., McSorley, H. J., Maizels, R. M., & Buck, A. H. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.05.001 · Cited by 219
Kim, Y.-J., Yoo, S. M., Park, H. H., Lim, H. J., Kim, Y.-L., Lee, S., Seo, K.-W., & Kang, K.-S. (2017) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2017.09.056 · Cited by 212
Nishida-Aoki, N., Tominaga, N., Takeshita, F., Sonoda, H., Yoshioka, Y., & Ochiya, T. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2016.10.009 · Cited by 208
Xia, X., Wang, Y., Huang, Y., Zhang, H., Lu, H., & Zheng, J. C. (2019) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2019.101694 · Cited by 202
You, Y., Borgmann, K., Edara, V. V., Stacy, S., Ghorpade, A., & Ikezu, T. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1706801 · Cited by 176
BMP-regulated exosomes from Drosophila male reproductive glands reprogram female behavior
Corrigan, L., Redhai, S., Leiblich, A., Fan, S.-J., Perera, S. M. W., Patel, R., Gandy, C., Wainwright, S. M., Morris, J. F., Hamdy, F., et al. (2014) · Journal of Cell Biology · doi:10.1083/jcb.201401072 · Cited by 155
Nooshabadi, V. T., Mardpour, S., Yousefi‐Ahmadipour, A., Allahverdi, A., Izadpanah, M., Daneshimehr, F., Ai, J., Banafshe, H. R., & Ebrahimi‐Barough, S. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26726 · Cited by 133
Microglia-derived extracellular vesicles in Alzheimer’s Disease: A double-edged sword
Trotta, T., Antonietta Panaro, M., Cianciulli, A., Mori, G., Di Benedetto, A., & Porro, C. (2018) · Biochemical Pharmacology · doi:10.1016/j.bcp.2017.12.020 · Cited by 126
Elashiry, M., Elashiry, M. M., Elsayed, R., Rajendran, M., Auersvald, C., Zeitoun, R., Rashid, M. H., Ara, R., Meghil, M. M., Liu, Y., et al. (2020) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2020.1795362 · Cited by 116
Harnessing the exosome-induced immune response for cancer immunotherapy
Gehrmann, U., Näslund, T. I., Hiltbrunner, S., Larssen, P., & Gabrielsson, S. (2014) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2014.05.003 · Cited by 111
Strategies for the use of Extracellular Vesicles for the Delivery of Therapeutics
Sil, S., Dagur, R. S., Liao, K., Peeples, E. S., Hu, G., Periyasamy, P., & Buch, S. (2020) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-019-09873-y · Cited by 109
Targeted Oral Delivery of Paclitaxel Using Colostrum-Derived Exosomes
Kandimalla, R., Aqil, F., Alhakeem, S., Jeyabalan, J., Tyagi, N., Agrawal, A., Yan, J., Spencer, W., Bondada, S., & Gupta, R. (2021) · Cancers · doi:10.3390/cancers13153700 · Cited by 99
Exosomes as Emerging Pro-Tumorigenic Mediators of the Senescence-Associated Secretory Phenotype
Jakhar, R., & Crasta, K. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20102547 · Cited by 98
Engineering strategies for customizing extracellular vesicle uptake in a therapeutic context
Esmaeili, A., Alini, M., Baghaban Eslaminejad, M., & Hosseini, S. (2022) · Stem Cell Research & Therapy · doi:10.1186/s13287-022-02806-2 · Cited by 94
Yang, L., Patel, K. D., Rathnam, C., Thangam, R., Hou, Y., Kang, H., & Lee, K. (2022) · Small · doi:10.1002/smll.202104783 · Cited by 81
Extracellular vesicles: translational challenges and opportunities
Clemmens, H., & Lambert, D. W. (2018) · Biochemical Society Transactions · doi:10.1042/bst20180112 · Cited by 70
Filannino, F. M., Panaro, M. A., Benameur, T., Pizzolorusso, I., & Porro, C. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25031629 · Cited by 60
Fuller, O. K., Whitham, M., Mathivanan, S., & Febbraio, M. A. (2020) · Cells · doi:10.3390/cells9102182 · Cited by 60
The Dichotomous Role of Extracellular Vesicles in the Central Nervous System
Graykowski, D. R., Wang, Y.-Z., Upadhyay, A., & Savas, J. N. (2020) · iScience · doi:10.1016/j.isci.2020.101456 · Cited by 39
Exosomes in cancer immunoediting and immunotherapy
Zhao, Y., Liu, L., Sun, R., Cui, G., Guo, S., Han, S., Li, Z., Bai, T., & Teng, L. (2022) · Asian Journal of Pharmaceutical Sciences · doi:10.1016/j.ajps.2021.12.001 · Cited by 39
Remyelinating effect driven by transferrin‐loaded extracellular vesicles
Mattera, V., Occhiuzzi, F., Correale, J., & Pasquini, J. M. (2024) · Glia · doi:10.1002/glia.24478 · Cited by 21
Ghosh, S., & Ghosh, S. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.878058 · Cited by 13
Extracellular Vesicles and Their Interplay with Biological Membranes
Yeo Xian Ping, J., Raj Neupane, Y., & Pastorin, G. (2022) · Physiology · doi:10.5772/intechopen.101297 · Cited by 9
Metadata
- Authors
- Morelli, A. E., Larregina, A. T., Shufesky, W. J., Sullivan, M. L. G., Stolz, D. B., Papworth, G. D., Zahorchak, A. F., Logar, A. J., Wang, Z., Watkins, S. C., Falo, L. D., & Thomson, A. W.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2004-11-15
- Volume
- 104
- Issue
- 10
- Pages
- 3257-3266
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2123614057
- Cited by
- 1,073
- References
- 2
- FWCI
- 7.11
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
