CoChat
Sign Up
Cited by 7,029Nitric Oxide and Endothelin EffectsRedox biology and oxidative stressNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.

Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide

Open access
Beckman, J S, Beckman, T W, Chen, J, Marshall, P A, Freeman, B A·1990·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.87.4.1620PMC PMC53527
Open this paper in CoChat — free account

Abstract

Superoxide dismutase reduces injury in many disease processes, implicating superoxide anion radical (O2-.) as a toxic species in vivo. A critical target of superoxide may be nitric oxide (NO.) produced by endothelium, macrophages, neutrophils, and brain synaptosomes. Superoxide and NO. are known to rapidly react to form the stable peroxynitrite anion (ONOO-). We have shown that peroxynitrite has a pKa of 7.49 +/- 0.06 at 37 degrees C and rapidly decomposes once protonated with a half-life of 1.9 sec at pH 7.4. Peroxynitrite decomposition generates a strong oxidant with reactivity similar to hydroxyl radical, as assessed by the oxidation of deoxyribose or dimethyl sulfoxide. Product yields indicative of hydroxyl radical were 5.1 +/- 0.1% and 24.3 +/- 1.0%, respectively, of added peroxynitrite. Product formation was not affected by the metal chelator diethyltriaminepentaacetic acid, suggesting that iron was not required to catalyze oxidation. In contrast, desferrioxamine was a potent, competitive inhibitor of peroxynitrite-initiated oxidation because of a direct reaction between desferrioxamine and peroxynitrite rather than by iron chelation. We propose that superoxide dismutase may protect vascular tissue stimulated to produce superoxide and NO. under pathological conditions by preventing the formation of peroxynitrite.

Overview

Integrity signals

Registry

Crossref · 10.1073/pnas.87.4.1620

Cites

25 of 25 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Citations

Cites

Flagged first, then by citations

Show all 25 references

Cited by

Sorted by citations

Items 101–199 of 199

Related

See all

Metadata

Authors
Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A.
Affiliations
  • University of Alabama at Birmingham (US)
  • Department of Anesthesiology, University of Alabama, Birmingham 35233.
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1990-02
Volume
87
Issue
4
Pages
1620-1624
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.87.4.1620
PMC
PMC53527
OpenAlex
W2058137293
Cited by
7,029
References
25
Citations by year
2026: 33 · 2025: 43 · 2024: 76 · 2023: 75 · 2022: 84 · 2021: 83 · 2020: 96 · 2019: 94 · 2018: 104 · 2017: 123 · 2016: 123 · 2015: 124 · 2014: 179 · 2013: 160 · 2012: 187
FWCI
67.23
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Heart Association
  • National Institutes of Health (5r01ns024275-04)
  • National Institute of Neurological Disorders and Stroke (NS 24338, NS24275)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
peroxynitrite, hydroxyl radicals, superoxide dismutase, nitric oxide, superoxide, endothelial injury, peroxynitrite decomposition, deferoxamine, deoxyribose oxidation, dimethyl sulfoxide oxidation, pKa, oxidative stress
MeSH terms
Animals, Endothelium, Vascular, Free Radicals, Half-Life, Hydrogen-Ion Concentration, Hydroxides, Kinetics, Models, Biological, Nitrates, Nitric Oxide, Peroxides, Superoxides, Superoxide Dismutase, Hydroxyl Radical

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review