The role of protein in weight loss and maintenance
Open accessAbstract
Over the past 20 y, higher-protein diets have been touted as a successful strategy to prevent or treat obesity through improvements in body weight management. These improvements are thought to be due, in part, to modulations in energy metabolism, appetite, and energy intake. Recent evidence also supports higher-protein diets for improvements in cardiometabolic risk factors. This article provides an overview of the literature that explores the mechanisms of action after acute protein consumption and the clinical health outcomes after consumption of long-term, higher-protein diets. Several meta-analyses of shorter-term, tightly controlled feeding studies showed greater weight loss, fat mass loss, and preservation of lean mass after higher-protein energy-restriction diets than after lower-protein energy-restriction diets. Reductions in triglycerides, blood pressure, and waist circumference were also reported. In addition, a review of the acute feeding trials confirms a modest satiety effect, including greater perceived fullness and elevated satiety hormones after higher-protein meals but does not support an effect on energy intake at the next eating occasion. Although shorter-term, tightly controlled feeding studies consistently identified benefits with increased protein consumption, longer-term studies produced limited and conflicting findings; nevertheless, a recent meta-analysis showed persistent benefits of a higher-protein weight-loss diet on body weight and fat mass. Dietary compliance appears to be the primary contributor to the discrepant findings because improvements in weight management were detected in those who adhered to the prescribed higher-protein regimen, whereas those who did not adhere to the diet had no marked improvements. Collectively, these data suggest that higher-protein diets that contain between 1.2 and 1.6 g protein · kg-1 · d-1 and potentially include meal-specific protein quantities of at least ∼25-30 g protein/meal provide improvements in appetite, body weight management, cardiometabolic risk factors, or all of these health outcomes; however, further strategies to increase dietary compliance with long-term dietary interventions are warranted.
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Higher-protein diets (≥25 percent of energy) enhance fullness, preserve lean mass, and boost resting energy expenditure versus standard protein levels.
“Higher-protein diets (≥25 percent of energy) enhance fullness, preserve lean mass, and boost resting energy expenditure versus standard protein levels [97].”
From Obesity: Clinical Impact, Pathophysiology, Complications, and Modern Innovations in Therapeutic Strategies (2025) · cites this paper as [97]
The sections on controlled higher-protein diets and the Conclusions report greater fullness and preservation of lean mass compared with lower-protein diets, particularly in shorter-term controlled studies. The mechanistic outcomes section says both diet groups’ resting energy expenditure fell during weight loss, but it fell less with higher protein; that supports preservation, not a boost. The paper includes diets with at least 25% of energy from protein, but does not establish ≥25% as a general cutoff for all three effects. (LLM, conf=0.88)
Experimental — accuracy not yet measuredHigher protein diets (1.2-1.6 g/kg of adjusted bodyweight versus standard 0.8 g/kg) may prevent metabolic adaptation.
“Higher protein diets (1.2–1.6 g/kg of adjusted bodyweight versus standard 0.8 g/kg) may prevent this type of metabolic adaptation”
From Lifestyle Medicine for Obesity in the Era of Highly Effective Anti-Obesity Treatment (2025) · cites this paper as [141]
The Mechanistic Outcomes section (page not provided) reports that higher-protein diets preserved resting energy expenditure better during weight loss, which supports the claim's general point about attenuating a metabolic decline. But resting energy expenditure fell on both diets; the paper does not establish prevention of metabolic adaptation or specify protein intake per kilogram of adjusted body weight. The Clinical Outcomes section (page not provided) compares protein ranges that include roughly 0.8 g/kg, rather than a single standard 0.8 g/kg diet. (LLM, conf=0.86)
Experimental — accuracy not yet measuredDietary protein positively influences body composition during weight loss and has a small positive effect on resting metabolism.
“In contrast to the near equivalency of dietary carbohydrate and fat, dietary protein is known to positively influence body composition during weight loss55,56 and has a small positive effect on resting metabolism57.”
From Maintenance of Lost Weight and Long-Term Management of Obesity (2018) · cites this paper as [55]
Higher protein targets, such as 1.2-1.6 g/kg/day, have been proposed during active weight reduction.
“Higher targets, such as 1.2–1.6 g/kg/day, have also been proposed during active weight reduction [162, 163].”
Many have contended that higher protein intakes are warranted during weight management, with preservation of fat-free mass and skeletal muscle being a commonly cited reason for this recommendation.
“While the recommended dietary allowance (RDA) for protein is 0.8 g·kg−1·day−1, expressed relative to BM, many have contended that higher protein intakes are warranted during weight management, with preservation of FFM and skeletal muscle being a commonly cited reason for this recommendation.24–27”
From Preservation of lean soft tissue during weight loss induced by GLP-1 and GLP-1/GIP receptor agonists: A case series (2025) · cites this paper as [27]
Intakes of 1.1 to 1.6 g/kg preserved FFM during weight loss better than 0.6 to 0.9 g/kg in individuals with overweight and obesity.
“Other research found that intakes of 1.1 to 1.6 g/kg preserved FFM during weight loss better than 0.6 to 0.9 g/kg in individuals with overweight and obesity [58, 59].”
From Fundamental Body Composition Principles Provide Context for Fat-Free and Skeletal Muscle Loss With GLP-1 RA Treatments (2024) · cites this paper as [59]
Higher protein intakes have additional potential benefits for appetite regulation, improving body composition, and influencing other health components.
“higher protein intakes have additional potential benefits for appetite regulation, improving body composition, and influencing other health components [59]”
From Fundamental Body Composition Principles Provide Context for Fat-Free and Skeletal Muscle Loss With GLP-1 RA Treatments (2024) · cites this paper as [59]
According to the American College of Sports Medicine, people with obesity undergoing weight loss treatment should consume approximately 1.25-1.5 times the Recommended Dietary Allowance for daily protein intake (for athletes >1.5), with at least ∼25-30 g of protein per meal to limit muscle mass loss.
“According to the American College of Sports Medicine, people with obesity undergoing WL treatment should consume approximately 1.25–1.5 times the Recommended Dietary Allowance (RDA) for daily protein intake (for athletes > 1.5), with at least ∼25–30 g of protein per meal [83,84] to limit muscle mass loss.”
From Nutrition and Physical Activity in Optimizing Weight Loss and Lean Mass Preservation in the Incretin-Based Medications Era: A Narrative Review (2025) · cites this paper as [83]
Increasing daily protein intake to 1.2-1.6 g/kg of body weight was associated with spontaneous reductions in energy intake under ad libitum conditions.
“In several studies, increasing daily protein intake to 1.2–1.6 g/kg of body weight was associated with spontaneous reductions in energy intake under ad libitum conditions [76,77,79].”
From Dietary and Behavioral Strategies for Weight Loss and Weight Loss Maintenance: A Narrative Review (2025) · cites this paper as [76]
Higher protein intake was associated with reduced hunger and greater satiety.
“In studies evaluating dietary composition, higher protein intake was associated with reduced hunger and greater satiety.”
From Dietary and Behavioral Strategies for Weight Loss and Weight Loss Maintenance: A Narrative Review (2025) · cites this paper as [76]
These effects coincided with changes in GLP-1, PYY, and ghrelin concentrations as well as increased thermogenesis.
“These effects coincided with changes in GLP-1, PYY, and ghrelin concentrations as well as increased thermogenesis [76,77].”
From Dietary and Behavioral Strategies for Weight Loss and Weight Loss Maintenance: A Narrative Review (2025) · cites this paper as [76]
Higher protein intake, ranging from 1.2 to 1.6 g per kilogram of body weight, has been repeatedly associated with reduced hunger, greater postprandial satiety, and spontaneous reduction in energy intake under ad libitum feeding conditions.
“Higher protein intake, ranging from 1.2 to 1.6 g per kilogram of body weight, has been repeatedly associated with reduced hunger, greater postprandial satiety, and spontaneous reduction in energy intake under ad libitum feeding conditions [76,77].”
From Dietary and Behavioral Strategies for Weight Loss and Weight Loss Maintenance: A Narrative Review (2025) · cites this paper as [76]
Higher protein targets, such as 1.2-1.6 g/kg/d, have been proposed during active weight reduction.
“Higher targets, such as 1.2–1.6 g/kg/d, have also been proposed during active weight reduction [162,163].”
Short-term trials showed that high-protein diets led to more weight loss, more fat mass loss, and better lean mass preservation than normal-protein diets.
“In short-term trials, consumption of high-protein diets have been shown to produce greater weight loss, loss of fat mass, and preservation of lean mass compared with the consumption of normal-protein diets.5,6”
From Association of Animal and Plant Protein Intake With All-Cause and Cause-Specific Mortality in a Japanese Cohort (2019) · cites this paper as [6]
Low-energy-dense foods such as vegetables and fruits are associated with greater satiety, and some evidence suggests that higher intakes of fiber and protein promote satiety.
“Low-energy-dense foods such as vegetables and fruits are associated with greater satiety, and some evidence suggests that higher intakes of fiber and protein promote satiety.62,63”
From Popular dietary patterns: alignment with American Heart Association 2021 dietary guidance: a scientific statement from the American Heart Association. (2023) · cites this paper as [63]
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Obesity and cancer risk: Emerging biological mechanisms and perspectives
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“preventing cancer in rodents and humans (157, 158).”
BackgroundDietary protein intake and human health
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Protein "requirements" beyond the RDA: implications for optimizing health
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Plant Protein and Animal Proteins: Do They Differentially Affect Cardiovascular Disease Risk?
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Gut microbiota, diet, and obesity-related disorders: the good, the bad, and the future challenges
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Commonly consumed protein foods contribute to nutrient intake, diet quality, and nutrient adequacy
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Successful weight loss maintenance: A systematic review of weight control registries
Paixão, C., Dias, C. M., Jorge, R., Carraça, E. V., Yannakoulia, M., de Zwaan, M., Soini, S., Hill, J. O., Teixeira, P. J., & Santos, I. (2020) · Obesity Reviews · doi:10.1111/obr.13003 · Cited by 158 · see citing works
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Current Concepts and Unresolved Questions in Dietary Protein Requirements and Supplements in Adults
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Mozaffarian, D., Agarwal, M., Aggarwal, M., Alexander, L., Apovian, C. M., Bindlish, S., Bonnet, J., Butsch, W. S., Christensen, S., Gianos, E., et al. (2025) · The American Journal of Clinical Nutrition · doi:10.1016/j.ajcnut.2025.04.023 · Cited by 154 · see citing works
“Further studies are warranted to elucidate the effects of GLP-1s on brain reward circuits and psychological dimensions of appetite and eating.”
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Low protein diets produce divergent effects on energy balance
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Obesity Alters the Muscle Protein Synthetic Response to Nutrition and Exercise
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“In addition to FFM and skeletal muscle preservation, higher protein intakes have additional potential benefits for appetite regulation, improving body composition, and influencing other health components [59], which could play a supportive role in GLP-1RA – induced weight loss.”
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The Effects of Different Quantities and Qualities of Protein Intake in People with Diabetes Mellitus
Pfeiffer, A. F. H., Pedersen, E., Schwab, U., Risérus, U., Aas, A.-M., Uusitupa, M., Thanopoulou, A., Kendall, C., Sievenpiper, J. L., Kahleová, H., et al. (2020) · Nutrients · doi:10.3390/nu12020365 · Cited by 60 · see citing works
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Protein- and Calcium-Mediated GLP-1 Secretion: A Narrative Review
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“Higher-protein diets ( ≥ 25 percent of energy) enhance fullness, preserve lean mass, and boost resting energy expenditure versus standard protein levels [97].”
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Dietary protein content for an optimal diet: a clinical view
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Metabolic Adaptations to Weight Loss: A Brief Review
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Self-citationLifestyle Medicine for Obesity in the Era of Highly Effective Anti-Obesity Treatment
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“- Elderly individuals, those undergoing weight loss, and those who are engaging in an intensive strength training regime may benefit from 1.2 g–1.6 g/kg of adjusted or ideal body weight [136,141,158].”
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Dietary and Behavioral Strategies for Weight Loss and Weight Loss Maintenance: A Narrative Review
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“27 However, there are limitations to considering protein intakes relative to BM in the context of obesity and substantial weight loss, and expressing intakes relative to FFM may be preferable in this context.”
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- Authors
- Leidy, H. J., Clifton, P. M., Astrup, A., Wycherley, T. P., Westerterp-Plantenga, M. S., Luscombe-Marsh, N. D., Woods, S. C., & Mattes, R. D.
- Affiliations
- University of Copenhagen (DK)
- Corresponding author
- Leidy, Heather J
- ORCID
- Venue
- The American Journal of Clinical Nutrition
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 101
- Issue
- 6
- Pages
- 1320S-1329S
- ISSN
- 0002-9165 (ISSN-L)
- OpenAlex
- W2121331954
- Semantic Scholar
- 1aaed2817d16bc4414dfa2d19003995b9982e5be
- Cited by
- 475
- References
- 96
- Citations by year
- 2026: 53 · 2025: 62 · 2024: 42 · 2023: 38 · 2022: 40 · 2021: 31 · 2020: 38 · 2019: 39 · 2018: 45 · 2017: 39 · 2016: 33 · 2015: 14
- FWCI
- 23.22
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- American Journal of Clinical Nutrition (publishedVersion)
- PubMed (publishedVersion)
- UniSA Research Outputs Repository (University of South Australia) (submittedVersion)
- Research Publications (Maastricht University) (submittedVersion, other-oa)
- Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Funders
- Beef Checkoff, Dairy Research Institute
- Egg Nutrition Center
- Global Dairy Platform, Hillshire Brands
- National Pork Board
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Physiology › Diet and metabolism studies
- Keywords
- high-protein diet, weight loss, weight maintenance, obesity, caloric restriction, satiety, appetite, lean mass preservation, fat loss, cardiometabolic risk factors, dietary adherence, energy metabolism
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