Cited by 475Muscle metabolism and nutritionRegulation of Appetite and ObesityDiet and metabolism studies

The role of protein in weight loss and maintenance

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Leidy, Heather J, Clifton, Peter M, Astrup, Arne, Wycherley, Thomas P, Westerterp-Plantenga, Margriet S, Luscombe-Marsh, Natalie D, Woods, Stephen C, Mattes, Richard D·2015·The American Journal of Clinical Nutrition

Abstract

Over the past 20 y, higher-protein diets have been touted as a successful strategy to prevent or treat obesity through improvements in body weight management. These improvements are thought to be due, in part, to modulations in energy metabolism, appetite, and energy intake. Recent evidence also supports higher-protein diets for improvements in cardiometabolic risk factors. This article provides an overview of the literature that explores the mechanisms of action after acute protein consumption and the clinical health outcomes after consumption of long-term, higher-protein diets. Several meta-analyses of shorter-term, tightly controlled feeding studies showed greater weight loss, fat mass loss, and preservation of lean mass after higher-protein energy-restriction diets than after lower-protein energy-restriction diets. Reductions in triglycerides, blood pressure, and waist circumference were also reported. In addition, a review of the acute feeding trials confirms a modest satiety effect, including greater perceived fullness and elevated satiety hormones after higher-protein meals but does not support an effect on energy intake at the next eating occasion. Although shorter-term, tightly controlled feeding studies consistently identified benefits with increased protein consumption, longer-term studies produced limited and conflicting findings; nevertheless, a recent meta-analysis showed persistent benefits of a higher-protein weight-loss diet on body weight and fat mass. Dietary compliance appears to be the primary contributor to the discrepant findings because improvements in weight management were detected in those who adhered to the prescribed higher-protein regimen, whereas those who did not adhere to the diet had no marked improvements. Collectively, these data suggest that higher-protein diets that contain between 1.2 and 1.6 g protein · kg-1 · d-1 and potentially include meal-specific protein quantities of at least ∼25-30 g protein/meal provide improvements in appetite, body weight management, cardiometabolic risk factors, or all of these health outcomes; however, further strategies to increase dietary compliance with long-term dietary interventions are warranted.

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Crossref · 10.3945/ajcn.114.084038

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  • Higher-protein diets (≥25 percent of energy) enhance fullness, preserve lean mass, and boost resting energy expenditure versus standard protein levels.

    “Higher-protein diets (≥25 percent of energy) enhance fullness, preserve lean mass, and boost resting energy expenditure versus standard protein levels [97].”

    From Obesity: Clinical Impact, Pathophysiology, Complications, and Modern Innovations in Therapeutic Strategies (2025) · cites this paper as [97]

    The sections on controlled higher-protein diets and the Conclusions report greater fullness and preservation of lean mass compared with lower-protein diets, particularly in shorter-term controlled studies. The mechanistic outcomes section says both diet groups’ resting energy expenditure fell during weight loss, but it fell less with higher protein; that supports preservation, not a boost. The paper includes diets with at least 25% of energy from protein, but does not establish ≥25% as a general cutoff for all three effects. (LLM, conf=0.88)

    Experimental — accuracy not yet measured
  • Higher protein diets (1.2-1.6 g/kg of adjusted bodyweight versus standard 0.8 g/kg) may prevent metabolic adaptation.

    “Higher protein diets (1.2–1.6 g/kg of adjusted bodyweight versus standard 0.8 g/kg) may prevent this type of metabolic adaptation”

    From Lifestyle Medicine for Obesity in the Era of Highly Effective Anti-Obesity Treatment (2025) · cites this paper as [141]

    The Mechanistic Outcomes section (page not provided) reports that higher-protein diets preserved resting energy expenditure better during weight loss, which supports the claim's general point about attenuating a metabolic decline. But resting energy expenditure fell on both diets; the paper does not establish prevention of metabolic adaptation or specify protein intake per kilogram of adjusted body weight. The Clinical Outcomes section (page not provided) compares protein ranges that include roughly 0.8 g/kg, rather than a single standard 0.8 g/kg diet. (LLM, conf=0.86)

    Experimental — accuracy not yet measured
  • Dietary protein positively influences body composition during weight loss and has a small positive effect on resting metabolism.

    “In contrast to the near equivalency of dietary carbohydrate and fat, dietary protein is known to positively influence body composition during weight loss55,56 and has a small positive effect on resting metabolism57.”
  • Higher protein targets, such as 1.2-1.6 g/kg/day, have been proposed during active weight reduction.

    “Higher targets, such as 1.2–1.6 g/kg/day, have also been proposed during active weight reduction [162, 163].”
  • Many have contended that higher protein intakes are warranted during weight management, with preservation of fat-free mass and skeletal muscle being a commonly cited reason for this recommendation.

    “While the recommended dietary allowance (RDA) for protein is 0.8 g·kg−1·day−1, expressed relative to BM, many have contended that higher protein intakes are warranted during weight management, with preservation of FFM and skeletal muscle being a commonly cited reason for this recommendation.24–27”

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Authors
Leidy, H. J., Clifton, P. M., Astrup, A., Wycherley, T. P., Westerterp-Plantenga, M. S., Luscombe-Marsh, N. D., Woods, S. C., & Mattes, R. D.
Affiliations
University of Copenhagen (DK)
Corresponding author
Leidy, Heather J
Venue
The American Journal of Clinical Nutrition
Publisher
Elsevier BV
Published
2015-06
Volume
101
Issue
6
Pages
1320S-1329S
ISSN
0002-9165 (ISSN-L)
OpenAlex
W2121331954
Cited by
475
References
96
Citations by year
2026: 53 · 2025: 62 · 2024: 42 · 2023: 38 · 2022: 40 · 2021: 31 · 2020: 38 · 2019: 39 · 2018: 45 · 2017: 39 · 2016: 33 · 2015: 14
FWCI
23.22
Type
journal article
Language
en
Open access
Locations
  • American Journal of Clinical Nutrition (publishedVersion)
  • PubMed (publishedVersion)
  • UniSA Research Outputs Repository (University of South Australia) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
  • Adelaide Research & Scholarship (AR&S) (University of Adelaide) (submittedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
  • Beef Checkoff, Dairy Research Institute
  • Egg Nutrition Center
  • Global Dairy Platform, Hillshire Brands
  • National Pork Board
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
  • Health Sciences › Medicine › Physiology › Diet and metabolism studies
Keywords
high-protein diet, weight loss, weight maintenance, obesity, caloric restriction, satiety, appetite, lean mass preservation, fat loss, cardiometabolic risk factors, dietary adherence, energy metabolism

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