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Cited by 273

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Skeletal myocytes are a source of interleukin‐6 mRNA expression and protein release during contraction: evidence of fiber type specificity

Hiscock, Natalie, Chan, M. H. Stanley, Bisucci, Teresa, Darby, Ian A., Febbraio, Mark A.·2004·The FASEB Journal
DOI 10.1096/fj.03-1259fje
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Abstract

In this study, we aimed to determine whether skeletal muscle cells per se are a source of interleukin (IL)‐6 during contraction and whether IL‐6 production is fiber type specific. Muscle biopsy samples were collected from seven males before (PRE) and after (POST) completing 120 min of continuous bicycle ergometry. Biopsies were sectioned and analyzed for the following: IL‐6 protein detected by immunohistochemistry (IHC), IL‐6 mRNA content detected by in situ hybridization, fiber type measured by either IHC or myofibrillar ATPase activity stain, and glycogen content measured by periodic acid schiff (PAS) assay. Fibers were qualitatively categorized according to glycogen content to one of five groups (1–5), with 1 being very low (LOW) and 5 being very high (HIGH) glycogen. Total fluorescence (PRE vs. POST) and glycogen‐dependent fluorescence (LOW vs. HIGH) of IL‐6 protein were quantitated using Metamorph software. Total IL‐6 protein was elevated from PRE to POST exercise ( P <0.05). At PRE, IL‐6 protein was evenly distributed across all fibers at low levels, consistent with glycogen distribution. At POST, IL‐6 protein was greater ( P <0.05) in HIGH compared with LOW glycogen fibers, which coincided with type 2 fibers. IL‐6 mRNA was distributed peripherally in all fibers at PRE. At POST, however, IL‐6 mRNA appeared predominantly in type 2 fibers, which also had higher glycogen content ( P <0.05). These data demonstrate that myocytes per se are a source of IL‐6 produced during contraction. Our data also suggest that type 2 fibers predominantly produce IL‐6 during muscle contractile activity.

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Metadata

Authors
Hiscock, N., Chan, M. H. S., Bisucci, T., Darby, I. A., & Febbraio, M. A.
Venue
The FASEB Journal
Publisher
Wiley
Published
2004-04
Volume
18
Issue
9
Pages
992-994
ISSN
  • 0892-6638 (ISSN-L)
  • 1530-6860
DOI
10.1096/fj.03-1259fje
OpenAlex
W2167753518
Cited by
273
References
3
FWCI
15.10
Type
journal article
Language
en
Open access
closed

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