A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing
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Fungal biofilms show higher antifungal resistance than planktonic populations.
“Fungal biofilms show properties different from planktonic (free-living) populations, including a higher antifungal resistance level [39].”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [39]
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- Authors
- Pierce, C. G., Uppuluri, P., Tristan, A. R., Wormley, F. L., Mowat, E., Ramage, G., & Lopez-Ribot, J. L.
- Affiliations
- The University of Texas at San Antonio (US)
- University of Glasgow (GB)
- Corresponding author
- Lopez-Ribot, Jose L
- Venue
- Nature Protocols
- Published
- 2008-08-28
- Volume
- 3
- Issue
- 9
- Pages
- 1494-1500
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- PMC
- PMC2741160
- OpenAlex
- W4253842255
- Cited by
- 1,041
- References
- 31
- Citations by year
- 2026: 53 · 2025: 62 · 2024: 69 · 2023: 71 · 2022: 90 · 2021: 68 · 2020: 86 · 2019: 102 · 2018: 83 · 2017: 91 · 2016: 73 · 2015: 47 · 2014: 44 · 2013: 33 · 2012: 23
- FWCI
- 9.69
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Protocols (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (R21DE017294)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- fungal biofilms, antifungal susceptibility testing, biofilm formation, 96-well plate, tetrazolium salt reduction, formazan, biofilm quantification, antifungal resistance, implant materials
- MeSH terms
- Formazans, Fungi, Biofilms, Cell Culture Techniques, Drug Resistance, Fungal
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