The mPer2 gene encodes a functional component of the mammalian circadian clock
Abstract
Circadian rhythms are driven by endogenous biological clocks that regulate many biochemical, physiological and behavioural processes in a wide range of life forms. In mammals, there is a master circadian clock in the suprachiasmatic nucleus of the anterior hypothalamus. Three putative mammalian homologues (mPer1, mPer2 and mPer3) of the Drosophila circadian clock gene period (per) have been identified. The mPer genes share a conserved PAS domain (a dimerization domain found in Per, Arnt and Sim) and show a circadian expression pattern in the suprachiasmatic nucleus. To assess the in vivo function of mPer2, we generated and characterized a deletion mutation in the PAS domain of the mouse mPer2 gene. Here we show that mice homozygous for this mutation display a shorter circadian period followed by a loss of circadian rhythmicity in constant darkness. The mutation also diminishes the oscillating expression of both mPer1 and mPer2 in the suprachiasmatic nucleus, indicating that mPer2 may regulate mPer1 in vivo. These data provide evidence that an mPer gene functions in the circadian clock, and define mPer2 as a component of the mammalian circadian oscillator.
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Loss of mPER2 function results in significantly reduced peak expression of mPer1, mPer2, and mCry1.
“loss of mPER2 function results in significantly reduced peak expression of mPer1, mPer2, and mCry1 (Shearman et al., 2000b; Zheng et al., 1999)”
From Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock. (2001) · cites this paper as Zheng et al., 1999
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- Authors
- Zheng, B., Larkin, D. W., Albrecht, U., Sun, Z. S., Sage, M., Eichele, G., Lee, C. C., & Bradley, A.
- Affiliations
- Baylor College of Medicine (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Lee, Cheng Chi
- Venue
- Nature
- Published
- 1999-07
- Volume
- 400
- Issue
- 6740
- Pages
- 169-173
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1629086938
- Cited by
- 718
- References
- 29
- Citations by year
- 2026: 16 · 2025: 21 · 2024: 22 · 2023: 19 · 2022: 28 · 2021: 28 · 2020: 25 · 2019: 17 · 2018: 28 · 2017: 31 · 2016: 24 · 2015: 20 · 2014: 32 · 2013: 35 · 2012: 20
- FWCI
- 14.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Keywords
- mPer2, circadian clock, period gene, PAS domain, suprachiasmatic nucleus, circadian period, circadian rhythmicity, mPer1, clock gene regulation, constant darkness
- MeSH terms
- Animals, Biological Clocks, Circadian Rhythm, Darkness, Drosophila, Fourier Analysis, Homozygote, Mice, Inbred C57BL, Molecular Sequence Data, Nuclear Proteins, Phenotype, Transcription Factors, Sequence Deletion, Cell Cycle Proteins, Reverse Transcriptase Polymerase Chain Reaction, Mice, Period Circadian Proteins
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