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Cited by 2,329Circadian rhythm and melatoninNonlinear Dynamics and Pattern FormationSleep and Work-Related Fatigue

PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues

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Yoo, Seung-Hee, Yamazaki, Shin, Lowrey, Phillip L., Shimomura, Kazuhiro, Ko, Caroline H., Buhr, Ethan D., Siepka, Sandra M., Hong, Hee-Kyung +4 more·2004·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0308709101PMC PMC397382
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Abstract

Mammalian circadian rhythms are regulated by the suprachiasmatic nucleus (SCN), and current dogma holds that the SCN is required for the expression of circadian rhythms in peripheral tissues. Using a PERIOD2::LUCIFERASE fusion protein as a real-time reporter of circadian dynamics in mice, we report that, contrary to previous work, peripheral tissues are capable of self-sustained circadian oscillations for >20 cycles in isolation. In addition, peripheral organs expressed tissue-specific differences in circadian period and phase. Surprisingly, lesions of the SCN in mPer2(Luciferase) knockin mice did not abolish circadian rhythms in peripheral tissues, but instead caused phase desynchrony among the tissues of individual animals and from animal to animal. These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level.

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Authors
Yoo, S.-H., Yamazaki, S., Lowrey, P. L., Shimomura, K., Ko, C. H., Buhr, E. D., Siepka, S. M., Hong, H.-K., Oh, W. J., Yoo, O. J., Menaker, M., & Takahashi, J. S.
Affiliations
Northwestern University (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2004-02-12
Volume
101
Issue
15
Pages
5339-5346
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0308709101
PMC
PMC397382
OpenAlex
W2014313900
Cited by
2,329
References
41
Citations by year
2026: 64 · 2025: 88 · 2024: 95 · 2023: 101 · 2022: 135 · 2021: 121 · 2020: 145 · 2019: 132 · 2018: 115 · 2017: 128 · 2016: 104 · 2015: 132 · 2014: 121 · 2013: 121 · 2012: 114
FWCI
29.04
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation
  • Howard Hughes Medical Institute
  • Korea Advanced Institute of Science and Technology
  • National Institutes of Health
  • National Institute of Mental Health (R01MH056647, MH 56647)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Physical Sciences › Computer Science › Computer Networks and Communications › Nonlinear Dynamics and Pattern Formation
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
Keywords
circadian rhythm, peripheral circadian rhythms, suprachiasmatic nucleus, circadian period, circadian phase
MeSH terms
Animals, Circadian Rhythm, Cornea, Female, Kidney, Liver, Luciferases, Luminescent Measurements, Lung, Male, Mice, Inbred C57BL, Mice, Transgenic, Motor Activity, Nuclear Proteins, Phenotype, Pituitary Gland, Recombinant Fusion Proteins, Suprachiasmatic Nucleus, Culture Techniques, Mice

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