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Cited by 8,116

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Genomic sequencing.

Open access
Church, G M, Gilbert, W·1984·Proceedings of the National Academy of Sciences

Abstract

Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence "ladder" extending from the 3' or 5' end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing. Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.

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Crossref · 10.1073/pnas.81.7.1991

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Metadata

Authors
Church, G. M., & Gilbert, W.
Venue
Proceedings of the National Academy of Sciences
Published
1984-04
Volume
81
Issue
7
Pages
1991-1995
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2576734941
Cited by
8,116
References
1
FWCI
56.10
Type
journal article
Language
en
Open access
green

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.