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Cited by 3,188

The DOI matches its Crossref record.

Quantitative Determination of Serum Triglycerides by the Use of Enzymes

Bucolo, Giovanni, David, Harold·1973·Clinical Chemistry
DOI 10.1093/clinchem/19.5.476
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Abstract

We describe a novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure. Under the conditions of the assay, the enzymatic hydrolysis can be completed in less than 10 min by the combined action of a microbial lipase and a protease. We have been able to demonstrate complete hydrolysis of triglycerides by thin-layer chromatography of the reaction products, by recovery of glycerol from sera of known triglycerides content, and by comparison of triglyceride assays on a number of sera assayed by our method vs. the AutoAnalyzer procedure. The hydrolysis is directly coupled to the enzymatic determination of glycerol, and is followed through absorbance changes at 340 nm. The assay is simple, rapid, and requires only 50 µl or less of sample. Because the enzymes used do not release glycerol from other compounds in serum, the hydrolysis can be considered specific for triglycerides.

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Metadata

Authors
Bucolo, G., & David, H.
Venue
Clinical Chemistry
Publisher
Oxford University Press (OUP)
Published
1973-05-01
Volume
19
Issue
5
Pages
476-482
ISSN
  • 0009-9147 (ISSN-L)
  • 1530-8561
DOI
10.1093/clinchem/19.5.476
OpenAlex
W2118313918
Cited by
3,188
References
0
FWCI
7.59
Type
journal article
Language
en
Open access
closed

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.