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Cited by 11,429

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Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

Open access
Muyzer, G, de Waal, E C, Uitterlinden, A G·1993·Applied and Environmental Microbiology

Abstract

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the separation pattern, which were most likely derived from as many different species constituting these populations, and thereby generated a DGGE profile of the populations. We showed that it is possible to identify constituents which represent only 1% of the total population. With an oligonucleotide probe specific for the V3 region of 16S rRNA of sulfate-reducing bacteria, particular DNA fragments from some of the microbial populations could be identified by hybridization analysis. Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment. The results we obtained demonstrate that this technique will contribute to our understanding of the genetic diversity of uncharacterized microbial populations.

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Crossref · 10.1128/aem.59.3.695-700.1993

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Metadata

Authors
Muyzer, G., de Waal, E. C., & Uitterlinden, A. G.
Venue
Applied and Environmental Microbiology
Published
1993-03
Volume
59
Issue
3
Pages
695-700
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2111858111
Cited by
11,429
References
1
FWCI
38.85
Type
journal article
Language
en
Open access
bronze

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