Organoids: The current status and biomedical applications

Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors

Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673

Embryonic Stem Cell Lines Derived from Human Blastocysts

Thomson, J. A., Itskovitz-Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,926

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204

Cerebral organoids model human brain development and microcephaly

Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372

Seria cultivation of strains of human epidemal keratinocytes: the formation keratinizin colonies from single cell is

Rheinwatd, J. G., & Green, H. (1975) · Cell · doi:10.1016/s0092-8674(75)80001-8 · Cited by 4,537

Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett’s epithelium

Sato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753

Organoid Models and Applications in Biomedical Research

Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · Nephron · doi:10.1159/000433566 · Cited by 3,515

Modeling Development and Disease with Organoids

Clevers, H. (2016) · Cell · doi:10.1016/j.cell.2016.05.082 · Cited by 3,196

Organogenesis in a dish: Modeling development and disease using organoid technologies

Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931

Non-alcoholic fatty liver disease

Powell, E. E., Wong, V. W.-S., & Rinella, M. (2021) · The Lancet · doi:10.1016/s0140-6736(20)32511-3 · Cited by 2,789

Targeting apoptosis in cancer therapy

Carneiro, B. A., & El-Deiry, W. S. (2020) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-020-0341-y · Cited by 2,542

Human organoids: model systems for human biology and medicine

Kim, J., Koo, B.-K., & Knoblich, J. A. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0259-3 · Cited by 2,187

Vascularized and functional human liver from an iPSC-derived organ bud transplant

Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

Vlachogiannis, G., Hedayat, S., Vatsiou, A., Jamin, Y., Fernández-Mateos, J., Khan, K., Lampis, A., Eason, K., Huntingford, I., Burke, R., et al. (2018) · Science · doi:10.1126/science.aao2774 · Cited by 2,096

Self-organizing optic-cup morphogenesis in three-dimensional culture

Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T., & Sasai, Y. (2011) · Nature · doi:10.1038/nature09941 · Cited by 2,001

A living biobank of breast cancer organoids captures disease heterogeneity

Sachs, N., de Ligt, J., Kopper, O., Gogola, E., Bounova, G., Weeber, F., Balgobind, A. V., Wind, K., Gracanin, A., Begthel, H., et al. (2018) · Cell · doi:10.1016/j.cell.2017.11.010 · Cited by 1,973

Organoids in cancer research

Drost, J., & Clevers, H. (2018) · Nature Reviews Cancer · doi:10.1038/s41568-018-0007-6 · Cited by 1,790

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

Baker, B. M., & Chen, C. S. (2012) · Journal of Cell Science · doi:10.1242/jcs.079509 · Cited by 1,760

Cancer immunoediting and resistance to T cell-based immunotherapy

O’Donnell, J. S., Teng, M. W. L., & Smyth, M. J. (2019) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-018-0142-8 · Cited by 1,759

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

Huch, M., Gehart, H., van Boxtel, R., Hamer, K., Blokzijl, F., Verstegen, M. M. A., Ellis, E., van Wenum, M., Fuchs, S. A., de Ligt, J., et al. (2015) · Cell · doi:10.1016/j.cell.2014.11.050 · Cited by 1,619

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

Gao, D., Vela, I., Sboner, A., Iaquinta, P. J., Karthaus, W. R., Gopalan, A., Dowling, C., Wanjala, J. N., Undvall, E. A., Arora, V. K., et al. (2014) · Cell · doi:10.1016/j.cell.2014.08.016 · Cited by 1,559

Organoid modeling of the tumor immune microenvironment

Neal, J. T., Li, X., Zhu, J., Giangarra, V., Grzeskowiak, C. L., Ju, J., Liu, I. H., Chiou, S.-H., Salahudeen, A. A., Smith, A. R., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.021 · Cited by 1,556

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

Barker, N., Huch, M., Kujala, P., van de Wetering, M., Snippert, H. J., van Es, J. H., Sato, T., Stange, D. E., Begthel, H., van den Born, M., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2009.11.013 · Cited by 1,547

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

Broutier, L., Mastrogiovanni, G., Verstegen, M. M., Francies, H. E., Gavarró, L. M., Bradshaw, C. R., Allen, G. E., Arnes-Benito, R., Sidorova, O., Gaspersz, M. P., et al. (2017) · Nature Medicine · doi:10.1038/nm.4438 · Cited by 1,471

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

Schwank, G., Koo, B.-K., Sasselli, V., Dekkers, J. F., Heo, I., Demircan, T., Sasaki, N., Boymans, S., Cuppen, E., van der Ent, C. K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.11.002 · Cited by 1,424

Neoadjuvant immunotherapy leads to pathological responses in MMR-proficient and MMR-deficient early-stage colon cancers

Chalabi, M., Fanchi, L. F., Dijkstra, K. K., Van den Berg, J. G., Aalbers, A. G., Sikorska, K., Lopez-Yurda, M., Grootscholten, C., Beets, G. L., Snaebjornsson, P., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-0805-8 · Cited by 1,417

Replication of human noroviruses in stem cell–derived human enteroids

Ettayebi, K., Crawford, S. E., Murakami, K., Broughman, J. R., Karandikar, U., Tenge, V. R., Neill, F. H., Blutt, S. E., Zeng, X.-L., Qu, L., et al. (2016) · Science · doi:10.1126/science.aaf5211 · Cited by 1,305

Engineering organoids

Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity

Jacob, F., Salinas, R. D., Zhang, D. Y., Nguyen, P. T. T., Schnoll, J. G., Wong, S. Z. H., Thokala, R., Sheikh, S., Saxena, D., Prokop, S., et al. (2020) · Cell · doi:10.1016/j.cell.2019.11.036 · Cited by 1,149

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

Dijkstra, K. K., Cattaneo, C. M., Weeber, F., Chalabi, M., van de Haar, J., Fanchi, L. F., Slagter, M., van der Velden, D. L., Kaing, S., Kelderman, S., et al. (2018) · Cell · doi:10.1016/j.cell.2018.07.009 · Cited by 1,135

Long‐term expanding human airway organoids for disease modeling

Sachs, N., Papaspyropoulos, A., Zomer‐van Ommen, D. D., Heo, I., Böttinger, L., Klay, D., Weeber, F., Huelsz‐Prince, G., Iakobachvili, N., Amatngalim, G. D., et al. (2019) · The EMBO Journal · doi:10.15252/embj.2018100300 · Cited by 1,082

Synthetic alternatives to Matrigel

Aisenbrey, E. A., & Murphy, W. L. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-0199-8 · Cited by 1,016

Modelling human development and disease in pluripotent stem-cell-derived gastric organoids

McCracken, K. W., Catá, E. M., Crawford, C. M., Sinagoga, K. L., Schumacher, M., Rockich, B. E., Tsai, Y.-H., Mayhew, C. N., Spence, J. R., Zavros, Y., et al. (2014) · Nature · doi:10.1038/nature13863 · Cited by 980

Tissue-specific mutation accumulation in human adult stem cells during life

Blokzijl, F., de Ligt, J., Jager, M., Sasselli, V., Roerink, S., Sasaki, N., Huch, M., Boymans, S., Kuijk, E., Prins, P., et al. (2016) · Nature · doi:10.1038/nature19768 · Cited by 935

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

Ootani, A., Li, X., Sangiorgi, E., Ho, Q. T., Ueno, H., Toda, S., Sugihara, H., Fujimoto, K., Weissman, I. L., Capecchi, M. R., et al. (2009) · Nature Medicine · doi:10.1038/nm.1951 · Cited by 906

Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer

Lee, S. H., Hu, W., Matulay, J. T., Silva, M. V., Owczarek, T. B., Kim, K., Chua, C. W., Barlow, L. J., Kandoth, C., Williams, A. B., et al. (2018) · Cell · doi:10.1016/j.cell.2018.03.017 · Cited by 873

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity

Kopper, O., de Witte, C. J., Lõhmussaar, K., Valle-Inclan, J. E., Hami, N., Kester, L., Balgobind, A. V., Korving, J., Proost, N., Begthel, H., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0422-6 · Cited by 834

NAFLD and increased risk of cardiovascular disease: clinical associations, pathophysiological mechanisms and pharmacological implications

Targher, G., Byrne, C. D., & Tilg, H. (2020) · Gut · doi:10.1136/gutjnl-2020-320622 · Cited by 827

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection

Bartfeld, S., Bayram, T., van de Wetering, M., Huch, M., Begthel, H., Kujala, P., Vries, R., Peters, P. J., & Clevers, H. (2015) · Gastroenterology · doi:10.1053/j.gastro.2014.09.042 · Cited by 820

Cell–extracellular matrix mechanotransduction in 3D

Saraswathibhatla, A., Indana, D., & Chaudhuri, O. (2023) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-023-00583-1 · Cited by 814

Human blood vessel organoids as a model of diabetic vasculopathy

Wimmer, R. A., Leopoldi, A., Aichinger, M., Wick, N., Hantusch, B., Novatchkova, M., Taubenschmid, J., Hämmerle, M., Esk, C., Bagley, J. A., et al. (2019) · Nature · doi:10.1038/s41586-018-0858-8 · Cited by 803

Fused cerebral organoids model interactions between brain regions

Bagley, J. A., Reumann, D., Bian, S., Lévi-Strauss, J., & Knoblich, J. A. (2017) · Nature Methods · doi:10.1038/nmeth.4304 · Cited by 790

Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer

Yao, Y., Xu, X., Yang, L., Zhu, J., Wan, J., Shen, L., Xia, F., Fu, G., Deng, Y., Pan, M., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2019.10.010 · Cited by 755

Limitations of Animal Studies for Predicting Toxicity in Clinical Trials

Van Norman, G. A. (2019) · JACC: Basic to Translational Science · doi:10.1016/j.jacbts.2019.10.008 · Cited by 732

Establishment of patient-derived cancer organoids for drug-screening applications

Driehuis, E., Kretzschmar, K., & Clevers, H. (2020) · Nature Protocols · doi:10.1038/s41596-020-0379-4 · Cited by 654

Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen

Xu, M., Lee, E. M., Wen, Z., Cheng, Y., Huang, W.-K., Qian, X., TCW, J., Kouznetsova, J., Ogden, S. C., Hammack, C., et al. (2016) · Nature Medicine · doi:10.1038/nm.4184 · Cited by 641

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

Workman, M. J., Mahe, M. M., Trisno, S., Poling, H. M., Watson, C. L., Sundaram, N., Chang, C.-F., Schiesser, J., Aubert, P., Stanley, E. G., et al. (2017) · Nature Medicine · doi:10.1038/nm.4233 · Cited by 626

Generation of Functional Human 3D Cortico-Motor Assembloids

Andersen, J., Revah, O., Miura, Y., Thom, N., Amin, N. D., Kelley, K. W., Singh, M., Chen, X., Thete, M. V., Walczak, E. M., et al. (2020) · Cell · doi:10.1016/j.cell.2020.11.017 · Cited by 593

Cardioids reveal self-organizing principles of human cardiogenesis

Hofbauer, P., Jahnel, S. M., Papai, N., Giesshammer, M., Deyett, A., Schmidt, C., Penc, M., Tavernini, K., Grdseloff, N., Meledeth, C., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.034 · Cited by 588

A brief history of organoids

Corrò, C., Novellasdemunt, L., & Li, V. S. W. (2020) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00120.2020 · Cited by 561

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537

Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease

Lewis-Israeli, Y. R., Wasserman, A. H., Gabalski, M. A., Volmert, B. D., Ming, Y., Ball, K. A., Yang, W., Zou, J., Ni, G., Pajares, N., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25329-5 · Cited by 536

Identification of SARS-CoV-2 inhibitors using lung and colonic organoids

Han, Y., Duan, X., Yang, L., Nilsson-Payant, B. E., Wang, P., Duan, F., Tang, X., Yaron, T. M., Zhang, T., Uhl, S., et al. (2021) · Nature · doi:10.1038/s41586-020-2901-9 · Cited by 530

Generation of human brain region–specific organoids using a miniaturized spinning bioreactor

Qian, X., Jacob, F., Song, M. M., Nguyen, H. N., Song, H., & Ming, G.-L. (2018) · Nature Protocols · doi:10.1038/nprot.2017.152 · Cited by 514

Human Organoids: Tools for Understanding Biology and Treating Diseases

Schutgens, F., & Clevers, H. (2020) · Annual Review of Pathology: Mechanisms of Disease · doi:10.1146/annurev-pathmechdis-012419-032611 · Cited by 510

Tissue geometry drives deterministic organoid patterning

Gjorevski, N., Nikolaev, M., Brown, T. E., Mitrofanova, O., Brandenberg, N., DelRio, F. W., Yavitt, F. M., Liberali, P., Anseth, K. S., & Lutolf, M. P. (2022) · Science · doi:10.1126/science.aaw9021 · Cited by 505

hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids

Xiang, Y., Tanaka, Y., Cakir, B., Patterson, B., Kim, K.-Y., Sun, P., Kang, Y.-J., Zhong, M., Liu, X., Patra, P., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2018.12.015 · Cited by 485

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

Takebe, T., Enomura, M., Yoshizawa, E., Kimura, M., Koike, H., Ueno, Y., Matsuzaki, T., Yamazaki, T., Toyohara, T., Osafune, K., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.03.004 · Cited by 457

A human breast cancer-derived xenograft and organoid platform for drug discovery and precision oncology

Guillen, K. P., Fujita, M., Butterfield, A. J., Scherer, S. D., Bailey, M. H., Chu, Z., DeRose, Y. S., Zhao, L., Cortes-Sanchez, E., Yang, C.-H., et al. (2022) · Nature Cancer · doi:10.1038/s43018-022-00337-6 · Cited by 416

Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells

Miura, Y., Li, M.-Y., Birey, F., Ikeda, K., Revah, O., Thete, M. V., Park, J.-Y., Puno, A., Lee, S. H., Porteus, M. H., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-00763-w · Cited by 415

Tumor organoid–T-cell coculture systems

Cattaneo, C. M., Dijkstra, K. K., Fanchi, L. F., Kelderman, S., Kaing, S., van Rooij, N., van den Brink, S., Schumacher, T. N., & Voest, E. E. (2020) · Nature Protocols · doi:10.1038/s41596-019-0232-9 · Cited by 410

Organoid Models of Tumor Immunology

Yuki, K., Cheng, N., Nakano, M., & Kuo, C. J. (2020) · Trends in Immunology · doi:10.1016/j.it.2020.06.010 · Cited by 402

Xenograft and organoid model systems in cancer research

Bleijs, M., van de Wetering, M., Clevers, H., & Drost, J. (2019) · The EMBO Journal · doi:10.15252/embj.2019101654 · Cited by 400

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

Fordham, R. P., Yui, S., Hannan, N. R. F., Soendergaard, C., Madgwick, A., Schweiger, P. J., Nielsen, O. H., Vallier, L., Pedersen, R. A., Nakamura, T., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.09.015 · Cited by 399

Diagnosis and Treatment of Alcohol-Associated Liver Disease: A Review

Singal, A. K., & Mathurin, P. (2021) · JAMA · doi:10.1001/jama.2021.7683 · Cited by 398

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

Cho, A.-N., Jin, Y., An, Y., Kim, J., Choi, Y. S., Lee, J. S., Kim, J., Choi, W.-Y., Koo, D.-J., Yu, W., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24775-5 · Cited by 397

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation

Sampaziotis, F., Cardoso de Brito, M., Madrigal, P., Bertero, A., Saeb-Parsy, K., Soares, F. A. C., Schrumpf, E., Melum, E., Karlsen, T. H., Bradley, J. A., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3275 · Cited by 397

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver

Sampaziotis, F., Muraro, D., Tysoe, O. C., Sawiak, S., Beach, T. E., Godfrey, E. M., Upponi, S. S., Brevini, T., Wesley, B. T., Garcia-Bernardo, J., et al. (2021) · Science · doi:10.1126/science.aaz6964 · Cited by 394

Glioblastoma Model Using Human Cerebral Organoids

Ogawa, J., Pao, G. M., Shokhirev, M. N., & Verma, I. M. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.03.105 · Cited by 391

Modelling Cryptosporidium infection in human small intestinal and lung organoids

Heo, I., Dutta, D., Schaefer, D. A., Iakobachvili, N., Artegiani, B., Sachs, N., Boonekamp, K. E., Bowden, G., Hendrickx, A. P. A., Willems, R. J. L., et al. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0177-8 · Cited by 389

High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids

Shinozawa, T., Kimura, M., Cai, Y., Saiki, N., Yoneyama, Y., Ouchi, R., Koike, H., Maezawa, M., Zhang, R.-R., Dunn, A., et al. (2021) · Gastroenterology · doi:10.1053/j.gastro.2020.10.002 · Cited by 378

Tissue extracellular matrix hydrogels as alternatives to Matrigel for culturing gastrointestinal organoids

Kim, S., Min, S., Choi, Y. S., Jo, S.-H., Jung, J. H., Han, K., Kim, J., An, S., Ji, Y. W., Kim, Y.-G., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29279-4 · Cited by 375

Engineering Stem Cell Self-organization to Build Better Organoids

Brassard, J. A., & Lutolf, M. P. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.05.005 · Cited by 373

Organoids in immunological research

Bar-Ephraim, Y. E., Kretzschmar, K., & Clevers, H. (2020) · Nature Reviews Immunology · doi:10.1038/s41577-019-0248-y · Cited by 361

Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics

Chen, K. G., Mallon, B. S., McKay, R. D. G., & Robey, P. G. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2013.12.005 · Cited by 358

Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids

Kim, H., Park, H. J., Choi, H., Chang, Y., Park, H., Shin, J., Kim, J., Lengner, C. J., Lee, Y. K., & Kim, J. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.01.020 · Cited by 346

Self-Organizing 3D Human Trunk Neuromuscular Organoids

Faustino Martins, J.-M., Fischer, C., Urzi, A., Vidal, R., Kunz, S., Ruffault, P.-L., Kabuss, L., Hube, I., Gazzerro, E., Birchmeier, C., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2019.12.007 · Cited by 336

Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture

Peng, W. C., Logan, C. Y., Fish, M., Anbarchian, T., Aguisanda, F., Álvarez-Varela, A., Wu, P., Jin, Y., Zhu, J., Li, B., et al. (2018) · Cell · doi:10.1016/j.cell.2018.11.012 · Cited by 331

Comprehensive metabolomics expands precision medicine for triple-negative breast cancer

Xiao, Y., Ma, D., Yang, Y.-S., Yang, F., Ding, J.-H., Gong, Y., Jiang, L., Ge, L.-P., Wu, S.-Y., Yu, Q., et al. (2022) · Cell Research · doi:10.1038/s41422-022-00614-0 · Cited by 326

Human brain organoid-on-a-chip to model prenatal nicotine exposure

Wang, Y., Wang, L., Zhu, Y., & Qin, J. (2018) · Lab on a Chip · doi:10.1039/c7lc01084b · Cited by 325

Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary

Koike, H., Iwasawa, K., Ouchi, R., Maezawa, M., Giesbrecht, K., Saiki, N., Ferguson, A., Kimura, M., Thompson, W. L., Wells, J. M., et al. (2019) · Nature · doi:10.1038/s41586-019-1598-0 · Cited by 325

Pluripotent stem cell-derived organoids: using principles of developmental biology to grow human tissues in a dish

McCauley, H. A., & Wells, J. M. (2017) · Development · doi:10.1242/dev.140731 · Cited by 322

A nomenclature consensus for nervous system organoids and assembloids

Pașca, S. P., Arlotta, P., Bateup, H. S., Camp, J. G., Cappello, S., Gage, F. H., Knoblich, J. A., Kriegstein, A. R., Lancaster, M. A., Ming, G.-L., et al. (2022) · Nature · doi:10.1038/s41586-022-05219-6 · Cited by 303

Multi-lineage human iPSC-derived platforms for disease modeling and drug discovery

Sharma, A., Sances, S., Workman, M. J., & Svendsen, C. N. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.02.011 · Cited by 296

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids

Sampaziotis, F., Justin, A. W., Tysoe, O. C., Sawiak, S., Godfrey, E. M., Upponi, S. S., Gieseck, R. L., de Brito, M. C., Berntsen, N. L., Gómez-Vázquez, M. J., et al. (2017) · Nature Medicine · doi:10.1038/nm.4360 · Cited by 296

Blood–brain-barrier organoids for investigating the permeability of CNS therapeutics

Bergmann, S., Lawler, S. E., Qu, Y., Fadzen, C. M., Wolfe, J. M., Regan, M. S., Pentelute, B. L., Agar, N. Y. R., & Cho, C.-F. (2018) · Nature Protocols · doi:10.1038/s41596-018-0066-x · Cited by 285

Identification of neural oscillations and epileptiform changes in human brain organoids

Samarasinghe, R. A., Miranda, O. A., Buth, J. E., Mitchell, S., Ferando, I., Watanabe, M., Allison, T. F., Kurdian, A., Fotion, N. N., Gandal, M. J., et al. (2021) · Nature Neuroscience · doi:10.1038/s41593-021-00906-5 · Cited by 281

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease

Ramli, M. N. B., Lim, Y. S., Koe, C. T., Demircioglu, D., Tng, W., Gonzales, K. A. U., Tan, C. P., Szczerbinska, I., Liang, H., Soe, E. L., et al. (2020) · Gastroenterology · doi:10.1053/j.gastro.2020.06.010 · Cited by 278

Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids

Dekkers, J. F., van Vliet, E. J., Sachs, N., Rosenbluth, J. M., Kopper, O., Rebel, H. G., Wehrens, E. J., Piani, C., Visvader, J. E., Verissimo, C. S., et al. (2021) · Nature Protocols · doi:10.1038/s41596-020-00474-1 · Cited by 272

A simple high-throughput approach identifies actionable drug sensitivities in patient-derived tumor organoids

Phan, N., Hong, J. J., Tofig, B., Mapua, M., Elashoff, D., Moatamed, N. A., Huang, J., Memarzadeh, S., Damoiseaux, R., & Soragni, A. (2019) · Communications Biology · doi:10.1038/s42003-019-0305-x · Cited by 269

Salmonella Manipulation of Host Signaling Pathways Provokes Cellular Transformation Associated with Gallbladder Carcinoma

Scanu, T., Spaapen, R. M., Bakker, J. M., Pratap, C. B., Wu, L.-E., Hofland, I., Broeks, A., Shukla, V. K., Kumar, M., Janssen, H., et al. (2015) · Cell Host & Microbe · doi:10.1016/j.chom.2015.05.002 · Cited by 266

SARS-CoV-2 infects the human kidney and drives fibrosis in kidney organoids

Jansen, J., Reimer, K. C., Nagai, J. S., Varghese, F. S., Overheul, G. J., de Beer, M., Roverts, R., Daviran, D., Fermin, L. A. S., Willemsen, B., et al. (2022) · Cell Stem Cell · doi:10.1016/j.stem.2021.12.010 · Cited by 259

Tumor Microenvironment-Derived NRG1 Promotes Antiandrogen Resistance in Prostate Cancer

Zhang, Z., Karthaus, W. R., Lee, Y. S., Gao, V. R., Wu, C., Russo, J. W., Liu, M., Mota, J. M., Abida, W., Linton, E., et al. (2020) · Cancer Cell · doi:10.1016/j.ccell.2020.06.005 · Cited by 259

Arid1a Has Context-Dependent Oncogenic and Tumor Suppressor Functions in Liver Cancer

Sun, X., Wang, S. C., Wei, Y., Luo, X., Jia, Y., Li, L., Gopal, P., Zhu, M., Nassour, I., Chuang, J.-C., et al. (2017) · Cancer Cell · doi:10.1016/j.ccell.2017.10.007 · Cited by 253

Reversed-engineered human alveolar lung-on-a-chip model

Huang, D., Liu, T., Liao, J., Maharjan, S., Xie, X., Pérez, M., Anaya, I., Wang, S., Tirado Mayer, A., Kang, Z., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2016146118 · Cited by 250

Human hepatic organoids for the analysis of human genetic diseases

Guan, Y., Xu, D., Garfin, P. M., Ehmer, U., Hurwitz, M., Enns, G., Michie, S., Wu, M., Zheng, M., Nishimura, T., et al. (2017) · JCI Insight · doi:10.1172/jci.insight.94954 · Cited by 243

L1CAM defines the regenerative origin of metastasis-initiating cells in colorectal cancer

Ganesh, K., Basnet, H., Kaygusuz, Y., Laughney, A. M., He, L., Sharma, R., O’Rourke, K. P., Reuter, V. P., Huang, Y.-H., Turkekul, M., et al. (2020) · Nature Cancer · doi:10.1038/s43018-019-0006-x · Cited by 242

Generation of Multicellular Tumor Spheroids by the Hanging-Drop Method

Timmins, N. E., & Nielsen, L. K. (2007) · Methods in Molecular Medicine™ · doi:10.1007/978-1-59745-443-8_8 · Cited by 241

An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping

Kawasaki, K., Toshimitsu, K., Matano, M., Fujita, M., Fujii, M., Togasaki, K., Ebisudani, T., Shimokawa, M., Takano, A., Takahashi, S., et al. (2020) · Cell · doi:10.1016/j.cell.2020.10.023 · Cited by 238

Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma

Saito, Y., Muramatsu, T., Kanai, Y., Ojima, H., Sukeda, A., Hiraoka, N., Arai, E., Sugiyama, Y., Matsuzaki, J., Uchida, R., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.03.088 · Cited by 230

An organoid‐derived bronchioalveolar model for SARS‐CoV‐2 infection of human alveolar type II‐like cells

Lamers, M. M., van der Vaart, J., Knoops, K., Riesebosch, S., Breugem, T. I., Mykytyn, A. Z., Beumer, J., Schipper, D., Bezstarosti, K., Koopman, C. D., et al. (2021) · The EMBO Journal · doi:10.15252/embj.2020105912 · Cited by 227

CRISPR-Based Adenine Editors Correct Nonsense Mutations in a Cystic Fibrosis Organoid Biobank

Geurts, M. H., de Poel, E., Amatngalim, G. D., Oka, R., Meijers, F. M., Kruisselbrink, E., van Mourik, P., Berkers, G., de Winter-de Groot, K. M., Michel, S., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.01.019 · Cited by 222

Wnt/β-catenin promotes gastric fundus specification in mice and humans

McCracken, K. W., Aihara, E., Martin, B., Crawford, C. M., Broda, T., Treguier, J., Zhang, X., Shannon, J. M., Montrose, M. H., & Wells, J. M. (2017) · Nature · doi:10.1038/nature21021 · Cited by 217

Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer

Lõhmussaar, K., Oka, R., Espejo Valle-Inclan, J., Smits, M. H. H., Wardak, H., Korving, J., Begthel, H., Proost, N., van de Ven, M., Kranenburg, O. W., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.03.012 · Cited by 211

Development of the liver: Insights into organ and tissue morphogenesis

Ober, E. A., & Lemaigre, F. P. (2018) · Journal of Hepatology · doi:10.1016/j.jhep.2018.01.005 · Cited by 211

Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury

Wang, S., Wang, X., Tan, Z., Su, Y., Liu, J., Chang, M., Yan, F., Chen, J., Chen, T., Li, C., et al. (2019) · Cell Research · doi:10.1038/s41422-019-0242-8 · Cited by 210

Assembling human brain organoids

Paşca, S. P. (2019) · Science · doi:10.1126/science.aau5729 · Cited by 208

Cerium Oxide Nanoparticles: Advances in Biodistribution, Toxicity, and Preclinical Exploration

Casals, E., Zeng, M., Parra‐Robert, M., Fernández‐Varo, G., Morales‐Ruiz, M., Jiménez, W., Puntes, V., & Casals, G. (2020) · Small · doi:10.1002/smll.201907322 · Cited by 204

Three-dimensional bioprinted hepatorganoids prolong survival of mice with liver failure

Yang, H., Sun, L., Pang, Y., Hu, D., Xu, H., Mao, S., Peng, W., Wang, Y., Xu, Y., Zheng, Y.-C., et al. (2021) · Gut · doi:10.1136/gutjnl-2019-319960 · Cited by 201

Modeling Sporadic Alzheimer's Disease in Human Brain Organoids under Serum Exposure

Chen, X., Sun, G., Tian, E., Zhang, M., Davtyan, H., Beach, T. G., Reiman, E. M., Blurton‐Jones, M., Holtzman, D. M., & Shi, Y. (2021) · Advanced Science · doi:10.1002/advs.202101462 · Cited by 200

Emerging organoid models: leaping forward in cancer research

Fan, H., Demirci, U., & Chen, P. (2019) · Journal of Hematology & Oncology · doi:10.1186/s13045-019-0832-4 · Cited by 199

Microfluidic organoids-on-a-chip: The future of human models

Saorin, G., Caligiuri, I., & Rizzolio, F. (2023) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2022.10.001 · Cited by 196

In Situ Patterning of Microfluidic Networks in 3D Cell‐Laden Hydrogels

Brandenberg, N., & Lutolf, M. P. (2016) · Advanced Materials · doi:10.1002/adma.201601099 · Cited by 192

A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®

Bircsak, K. M., DeBiasio, R., Miedel, M., Alsebahi, A., Reddinger, R., Saleh, A., Shun, T., Vernetti, L. A., & Gough, A. (2021) · Toxicology · doi:10.1016/j.tox.2020.152667 · Cited by 192

Reprogramming Cellular Identity for Regenerative Medicine

Cherry, A. B. C., & Daley, G. Q. (2012) · Cell · doi:10.1016/j.cell.2012.02.031 · Cited by 191

A human liver organoid screening platform for DILI risk prediction

Zhang, C. J., Meyer, S. R., O’Meara, M. J., Huang, S., Capeling, M. M., Ferrer-Torres, D., Childs, C. J., Spence, J. R., Fontana, R. J., & Sexton, J. Z. (2023) · Journal of Hepatology · doi:10.1016/j.jhep.2023.01.019 · Cited by 191

Microengineered perfusable 3D-bioprinted glioblastoma model for in vivo mimicry of tumor microenvironment

Neufeld, L., Yeini, E., Reisman, N., Shtilerman, Y., Ben-Shushan, D., Pozzi, S., Madi, A., Tiram, G., Eldar-Boock, A., Ferber, S., et al. (2021) · Science Advances · doi:10.1126/sciadv.abi9119 · Cited by 189

Patient-specific modeling of stroma-mediated chemoresistance of pancreatic cancer using a three-dimensional organoid-fibroblast co-culture system

Schuth, S., Blanc, S. L., Krieger, T. G., Jabs, J., Schenk, M., Giese, N. A., Büchler, M. W., Eils, R., Conrad, C., & Strobel, O. (2022) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-022-02519-7 · Cited by 189

3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert

Shin, W., & Kim, H. J. (2022) · Nature Protocols · doi:10.1038/s41596-021-00674-3 · Cited by 185

Gene-edited human stem cell–derived β cells from a patient with monogenic diabetes reverse preexisting diabetes in mice

Maxwell, K. G., Augsornworawat, P., Velazco-Cruz, L., Kim, M. H., Asada, R., Hogrebe, N. J., Morikawa, S., Urano, F., & Millman, J. R. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.aax9106 · Cited by 183

Modeling glioblastoma invasion using human brain organoids and single-cell transcriptomics

Krieger, T. G., Tirier, S. M., Park, J., Jechow, K., Eisemann, T., Peterziel, H., Angel, P., Eils, R., & Conrad, C. (2020) · Neuro-Oncology · doi:10.1093/neuonc/noaa091 · Cited by 181

Understanding the role of tissue-specific decellularized spinal cord matrix hydrogel for neural stem/progenitor cell microenvironment reconstruction and spinal cord injury

Xu, Y., Zhou, J., Liu, C., Zhang, S., Gao, F., Guo, W., Sun, X., Zhang, C., Li, H., Rao, Z., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120596 · Cited by 179

Potassium channel dysfunction in human neuronal models of Angelman syndrome

Sun, A. X., Yuan, Q., Fukuda, M., Yu, W., Yan, H., Lim, G. G. Y., Nai, M. H., D’Agostino, G. A., Tran, H.-D., Itahana, Y., et al. (2019) · Science · doi:10.1126/science.aav5386 · Cited by 178

An organoid-based organ-repurposing approach to treat short bowel syndrome

Sugimoto, S., Kobayashi, E., Fujii, M., Ohta, Y., Arai, K., Matano, M., Ishikawa, K., Miyamoto, K., Toshimitsu, K., Takahashi, S., et al. (2021) · Nature · doi:10.1038/s41586-021-03247-2 · Cited by 175

Modeling medulloblastoma in vivo and with human cerebellar organoids

Ballabio, C., Anderle, M., Gianesello, M., Lago, C., Miele, E., Cardano, M., Aiello, G., Piazza, S., Caron, D., Gianno, F., et al. (2020) · Nature Communications · doi:10.1038/s41467-019-13989-3 · Cited by 175

CRISPR-Cas9–mediated gene knockout in intestinal tumor organoids provides functional validation for colorectal cancer driver genes

Takeda, H., Kataoka, S., Nakayama, M., Ali, M. A. E., Oshima, H., Yamamoto, D., Park, J.-W., Takegami, Y., An, T., Jenkins, N. A., et al. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904714116 · Cited by 172

A human tissue screen identifies a regulator of ER secretion as a brain-size determinant

Esk, C., Lindenhofer, D., Haendeler, S., Wester, R. A., Pflug, F., Schroeder, B., Bagley, J. A., Elling, U., Zuber, J., von Haeseler, A., et al. (2020) · Science · doi:10.1126/science.abb5390 · Cited by 170

Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver‐Specific Microenvironment as a Drug Testing Platform

Jin, Y., Kim, J., Lee, J. S., Min, S., Kim, S., Ahn, D., Kim, Y., & Cho, S. (2018) · Advanced Functional Materials · doi:10.1002/adfm.201801954 · Cited by 168

Cystic Fibrosis

Dickinson, K. M., & Collaco, J. M. (2021) · Pediatrics in Review · doi:10.1542/pir.2019-0212 · Cited by 167

Amplification of human interneuron progenitors promotes brain tumors and neurological defects

Eichmüller, O. L., Corsini, N. S., Vértesy, Á., Morassut, I., Scholl, T., Gruber, V.-E., Peer, A. M., Chu, J., Novatchkova, M., Hainfellner, J. A., et al. (2022) · Science · doi:10.1126/science.abf5546 · Cited by 163

Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis

Anada, T., Pan, C., Stahl, A. M., Mori, S., Fukuda, J., Suzuki, O., & Yang, Y. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20051096 · Cited by 161

Application of human liver organoids as a patient-derived primary model for HBV infection and related hepatocellular carcinoma

Crignis, E. D., Hossain, T., Romal, S., Carofiglio, F., Moulos, P., Khalid, M. M., Rao, S., Bazrafshan, A., Verstegen, M. M. A., Pourfarzad, F., et al. (2021) · eLife · doi:10.7554/elife.60747 · Cited by 160

Modelling liver cancer initiation with organoids derived from directly reprogrammed human hepatocytes

Sun, L., Wang, Y., Cen, J., Ma, X., Cui, L., Qiu, Z., Zhang, Z., Li, H., Yang, R.-Z., Wang, C., et al. (2019) · Nature Cell Biology · doi:10.1038/s41556-019-0359-5 · Cited by 159

Patient-derived organoids and orthotopic xenografts of primary and recurrent gliomas represent relevant patient avatars for precision oncology

Golebiewska, A., Hau, A.-C., Oudin, A., Stieber, D., Yabo, Y. A., Baus, V., Barthelemy, V., Klein, E., Bougnaud, S., Keunen, O., et al. (2020) · Acta Neuropathologica · doi:10.1007/s00401-020-02226-7 · Cited by 159

Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver

Hendriks, D., Artegiani, B., Hu, H., Chuva de Sousa Lopes, S., & Clevers, H. (2021) · Nature Protocols · doi:10.1038/s41596-020-00411-2 · Cited by 159

Organoid modeling of Zika and herpes simplex virus 1 infections reveals virus-specific responses leading to microcephaly

Krenn, V., Bosone, C., Burkard, T. R., Spanier, J., Kalinke, U., Calistri, A., Salata, C., Rilo Christoff, R., Pestana Garcez, P., Mirazimi, A., et al. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.03.004 · Cited by 159

Establishment of a heart-on-a-chip microdevice based on human iPS cells for the evaluation of human heart tissue function

Abulaiti, M., Yalikun, Y., Murata, K., Sato, A., Sami, M. M., Sasaki, Y., Fujiwara, Y., Minatoya, K., Shiba, Y., Tanaka, Y., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-76062-w · Cited by 158

Generation of model tissues with dendritic vascular networks via sacrificial laser-sintered carbohydrate templates

Kinstlinger, I. S., Saxton, S. H., Calderon, G. A., Ruiz, K. V., Yalacki, D. R., Deme, P. R., Rosenkrantz, J. E., Louis-Rosenberg, J. D., Johansson, F., Janson, K. D., et al. (2020) · Nature Biomedical Engineering · doi:10.1038/s41551-020-0566-1 · Cited by 154

Viral hepatitis: Past, present, and future

Odenwald, M. A., & Paul, S. (2022) · World Journal of Gastroenterology · doi:10.3748/wjg.v28.i14.1405 · Cited by 153

Modeling Clinical Responses to Targeted Therapies by Patient-Derived Organoids of Advanced Lung Adenocarcinoma

Kim, S.-Y., Kim, S.-M., Lim, S., Lee, J. Y., Choi, S.-J., Yang, S.-D., Yun, M. R., Kim, C. G., Gu, S. R., Park, C., et al. (2021) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-20-5026 · Cited by 151

A human multi-lineage hepatic organoid model for liver fibrosis

Guan, Y., Enejder, A., Wang, M., Fang, Z., Cui, L., Chen, S.-Y., Wang, J., Tan, Y., Wu, M., Chen, X., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-26410-9 · Cited by 149

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

Li, Z., Qian, Y., Li, W., Liu, L., Yu, L., Liu, X., Wu, G., Wang, Y., Luo, W., Fang, F., et al. (2020) · iScience · doi:10.1016/j.isci.2020.101411 · Cited by 147

3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform

Candiello, J., Grandhi, T. S. P., Goh, S. K., Vaidya, V., Lemmon-Kishi, M., Eliato, K. R., Ros, R., Kumta, P. N., Rege, K., & Banerjee, I. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.031 · Cited by 146

High-affinity neoantigens correlate with better prognosis and trigger potent antihepatocellular carcinoma (HCC) activity by activating CD39 + CD8 + T cells

Liu, T., Tan, J., Wu, M., Fan, W., Wei, J., Zhu, B., Guo, J. M., Wang, S., Zhou, P., Zhang, H., et al. (2020) · Gut · doi:10.1136/gutjnl-2020-322196 · Cited by 146

Organoids in gastrointestinal diseases: From experimental models to clinical translation

Günther, C., Winner, B., Neurath, M. F., & Stappenbeck, T. S. (2022) · Gut · doi:10.1136/gutjnl-2021-326560 · Cited by 145

Structural and functional integration of human forebrain organoids with the injured adult rat visual system

Jgamadze, D., Lim, J. T., Zhang, Z., Harary, P. M., Germi, J., Mensah-Brown, K. G., Adam, C. D., Mirzakhalili, E., Singh, S., Gu, J. B., et al. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.01.004 · Cited by 144

DSCAM/PAK1 pathway suppression reverses neurogenesis deficits in iPSC-derived cerebral organoids from patients with Down syndrome

Tang, X.-Y., Xu, L., Wang, J., Hong, Y., Wang, Y., Zhu, Q., Wang, D., Zhang, X.-Y., Liu, C.-Y., Fang, K.-H., et al. (2021) · Journal of Clinical Investigation · doi:10.1172/jci135763 · Cited by 143

Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure

Meran, L., Massie, I., Campinoti, S., Weston, A. E., Gaifulina, R., Tullie, L., Faull, P., Orford, M., Kucharska, A., Baulies, A., et al. (2020) · Nature Medicine · doi:10.1038/s41591-020-1024-z · Cited by 143

Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors

Ribeiro, J., Procyk, C. A., West, E. L., O’Hara-Wright, M., Martins, M. F., Khorasani, M. M., Hare, A., Basche, M., Fernando, M., Goh, D., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109022 · Cited by 143

An Organoid-Based Preclinical Model of Human Gastric Cancer

Steele, N. G., Chakrabarti, J. L., Wang, J., Biesiada, J., Holokai, L. L., Chang, J., Nowacki, L. M., Hawkins, J., Mahé, M. M., Sundaram, N., et al. (2018) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2018.09.008 · Cited by 142

Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells

Eicher, A. K., Kechele, D. O., Sundaram, N., Berns, H. M., Poling, H. M., Haines, L. E., Sanchez, J. G., Kishimoto, K., Krishnamurthy, M., Han, L., et al. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.10.010 · Cited by 142

A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discovery

Tran, T., Song, C. J., Nguyen, T. T. T., Cheng, S.-Y., McMahon, J. A., Yang, R., Guo, Q., Dér, B., Lindström, N. O., Lin, D. C.-H., et al. (2022) · Cell stem cell · doi:10.1016/j.stem.2022.06.005 · Cited by 142

Paracrine signals regulate human liver organoid maturation from iPSC

Asai, A., Aihara, E., Watson, C. L., Mourya, R., Mizuochi, T., Shivakumar, P., Phelan, K. J., Mayhew, C. N., Helmrath, M. A., Takebe, T., et al. (2017) · Development · doi:10.1242/dev.142794 · Cited by 141

Revealing Tissue-Specific SARS-CoV-2 Infection and Host Responses using Human Stem Cell-Derived Lung and Cerebral Organoids

Tiwari, S. K., Wang, S., Smith, D. M., Carlin, A. F., & Rana, T. M. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.02.005 · Cited by 140

Organoid Sensitivity Correlates with Therapeutic Response in Patients with Pancreatic Cancer

Grossman, J. E., Muthuswamy, L. B., Huang, L., Akshinthala, D., Perea, S., González, R. S., Tsai, L. L., Cohen, J., Bockorny, B., Bullock, A. J., et al. (2021) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-20-4116 · Cited by 139

Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures

Berger-Müller, S., Ader, I., Creff, J., Leménager, H., Achard, P., Casteilla, L., Sensebé, L., Carrière, A., & Deschaseaux, F. (2019) · Scientific Reports · doi:10.1038/s41598-019-43624-6 · Cited by 138

New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids

Ettayebi, K., Tenge, V. R., Cortes-Penfield, N. W., Crawford, S. E., Neill, F. H., Zeng, X., Yu, X., Ayyar, B. V., Burrin, D. G., Ramani, S., et al. (2021) · mSphere · doi:10.1128/msphere.01136-20 · Cited by 135

Biomimetic hydrogel supports initiation and growth of patient-derived breast tumor organoids

Prince, E., Cruickshank, J., Ba-Alawi, W., Hodgson, K., Haight, J., Tobin, C., Wakeman, A., Avoulov, A., Topolskaia, V., Elliott, M. J., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-28788-6 · Cited by 135

Single‐Cell Transcriptome Analysis Uncovers Intratumoral Heterogeneity and Underlying Mechanisms for Drug Resistance in Hepatobiliary Tumor Organoids

Zhao, Y., Li, Z., Zhu, Y., Fu, J., Zhao, X., Zhang, Y., Wang, S., Wu, J., Wang, K., Wu, R. T., et al. (2021) · Advanced Science · doi:10.1002/advs.202003897 · Cited by 134

Exploring the human lacrimal gland using organoids and single-cell sequencing

Bannier-Hélaouët, M., Post, Y., Korving, J., Bustos, M. T., Gehart, H., Begthel, H., Bar‐Ephraïm, Y. E., van der Vaart, J., Kalmann, R., Imhoff, S. M., et al. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.02.024 · Cited by 134

Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders

Mun, K. S., Arora, K., Huang, Y., Yang, F., Yarlagadda, S., Ramananda, Y., Abu‐El‐Haija, M. A., Palermo, J. J., Balamurugan, A. N., Nathan, J. D., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11178-w · Cited by 130

Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids

Huang, L., Desai, R., Conrad, D. N., Leite, N. C., Akshinthala, D., Lim, C. M., González, R. S., Muthuswamy, L. B., Gartner, Z. J., & Muthuswamy, S. K. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.03.022 · Cited by 128

Human cerebral organoids establish subcortical projections in the mouse brain after transplantation

Dong, X., Xu, S., Chen, X., Tao, M., Tang, X.-Y., Fang, K.-H., Xu, M., Pan, Y., Chen, Y., He, S., et al. (2020) · Molecular Psychiatry · doi:10.1038/s41380-020-00910-4 · Cited by 127

Third-line therapy for chronic myeloid leukemia: current status and future directions

Cortes, J. E., & Lang, F. (2021) · Journal of Hematology & Oncology · doi:10.1186/s13045-021-01055-9 · Cited by 124

Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair

Gupta, N. R., Matsumoto, T., Hiratsuka, K., Saiz, E. G., Zhang, C., Galichon, P., Miyoshi, T., Susa, K., Tatsumoto, N., Yamashita, M., et al. (2022) · Science Translational Medicine · doi:10.1126/scitranslmed.abj4772 · Cited by 124

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

Tysoe, O., Justin, A. W., Brevini, T., Chen, S. E., Mahbubani, K. T. A., Frank, A. K., Zedira, H., Melum, E., Saeb‐Parsy, K., Markaki, A. E., et al. (2019) · Nature Protocols · doi:10.1038/s41596-019-0168-0 · Cited by 123

Patient‐derived renal cell carcinoma organoids for personalized cancer therapy

Li, Z., Xu, H., Yu, L., Wang, J., Meng, Q., Mei, H., Cai, Z., Chen, W., & Huang, W. (2022) · Clinical and Translational Medicine · doi:10.1002/ctm2.970 · Cited by 122

Correction of CFTR function in intestinal organoids to guide treatment of cystic fibrosis

Ramalho, A. S., Fürstová, E., Vonk, A. M., Ferrante, M., Verfaillie, C., Dupont, L., Boon, M., Proesmans, M., Beekman, J. M., Sarouk, I., et al. (2021) · European Respiratory Journal · doi:10.1183/13993003.02426-2019 · Cited by 121

Downregulation of TGR5 (GPBAR1) in biliary epithelial cells contributes to the pathogenesis of sclerosing cholangitis

Reich, M., Spomer, L., Klindt, C., Fuchs, K., Stindt, J., Deutschmann, K., Höhne, J., Liaskou, E., Hov, J. R., Karlsen, T. H., et al. (2021) · Journal of Hepatology · doi:10.1016/j.jhep.2021.03.029 · Cited by 121

Disease Modeling and Gene Therapy of Copper Storage Disease in Canine Hepatic Organoids

Assawarachan, S. N., Spee, B., Kruitwagen, H. S., Chen, C., Geijsen, N., Oosterhoff, L. A., van Wolferen, M. E., Pelaez-Llaneza, N., Fieten, H., Wubbolts, R., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.09.002 · Cited by 119

Retinoblastoma from human stem cell-derived retinal organoids

Norrie, J. L., Nityanandam, A., Lai, K., Chen, X. '., Wilson, M. W., Stewart, E. A., Griffiths, L. M., Jin, H., Wu, G., Orr, B. A., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24781-7 · Cited by 119

Gene Therapy of Dominant CRX-Leber Congenital Amaurosis using Patient Stem Cell-Derived Retinal Organoids

Kruczek, K., Qu, Z., Gentry, J., Fadl, B. R., Gieser, L. M., Hiriyanna, S., Batz, Z. A., Samant, M. D., Samanta, A., Chu, C. J., et al. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.12.018 · Cited by 117

Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells

Breunig, M., Merkle, J., Wagner, M., Melzer, M. K., Barth, T. F. E., Engleitner, T., Krumm, J., Wiedenmann, S., Cohrs, C. M., Perkhofer, L., et al. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.03.005 · Cited by 117

En masse organoid phenotyping informs metabolic-associated genetic susceptibility to NASH

Kimura, M., Iguchi, T., Iwasawa, K., Dunn, A., Thompson, W. L., Yoneyama, Y., Chaturvedi, P., Zorn, A. M., Wintzinger, M., Quattrocelli, M., et al. (2022) · Cell · doi:10.1016/j.cell.2022.09.031 · Cited by 115

Brain organoid formation on decellularized porcine brain ECM hydrogels

Simsa, R., Rothenbücher, T., Gürbüz, H., Ghosheh, N., Emneus, J., Jenndahl, L., Kaplan, D. L., Bergh, N., Serrano, A. M., & Fogelstrand, P. (2021) · PLOS ONE · doi:10.1371/journal.pone.0245685 · Cited by 114

Environmental Toxin Screening Using Human-Derived 3D Bioengineered Liver and Cardiac Organoids

Forsythe, S. D., Devarasetty, M., Shupe, T., Bishop, C., Atala, A., Soker, S., & Skardal, A. (2018) · Frontiers in Public Health · doi:10.3389/fpubh.2018.00103 · Cited by 112

Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids

Georgakopoulos, N., Prior, N., Angres, B., Mastrogiovanni, G., Cagan, A., Harrison, D., Hindley, C. J., Arnes-Benito, R., Liau, S.-S., Curd, A., et al. (2020) · BMC Developmental Biology · doi:10.1186/s12861-020-0209-5 · Cited by 112

Drug Inhibition of SARS-CoV-2 Replication in Human Pluripotent Stem Cell–Derived Intestinal Organoids

Krüger, J., Groß, R. M., Conzelmann, C., Müller, J. A., Koepke, L., Sparrer, K. M. J., Weil, T., Schütz, D., Seufferlein, T. T. W., Barth, T. F. E., et al. (2020) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2020.11.003 · Cited by 109

Pancreatic Cancer Patient-derived Organoids Can Predict Response to Neoadjuvant Chemotherapy

Demyan, L., Habowski, A. N., Plenker, D., King, D. A., Standring, O. J., Tsang, C., Surin, L. S., Rishi, A., Crawford, J. M., Boyd, J., et al. (2022) · Annals of Surgery · doi:10.1097/sla.0000000000005558 · Cited by 109

Hydrogels derived from decellularized liver tissue support the growth and differentiation of cholangiocyte organoids

Willemse, J., van Tienderen, G., van Hengel, E., Schurink, I., van der Ven, D., Kan, Y., de Ruiter, P., Rosmark, O., Westergren-Thorsson G, G., Schneeberger, K., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121473 · Cited by 106

Precision Medicine in Pancreatic Cancer: Patient-Derived Organoid Pharmacotyping Is a Predictive Biomarker of Clinical Treatment Response

Seppälä, T. T., Zimmerman, J. W., Suri, R., Zlomke, H., Ivey, G. D., Szabolcs, A., Shubert, C. R., Cameron, J. L., Burns, W. R., Lafaro, K. J., et al. (2022) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-21-4165 · Cited by 106

The cholesterol uptake regulator PCSK9 promotes and is a therapeutic target in APC/KRAS-mutant colorectal cancer

Wong, C. C., Wu, J.-L., Ji, F., Kang, W., Bian, X., Chen, H., Chan, L.-S., Luk, S. T. Y., Tong, S., Xu, J., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-31663-z · Cited by 103

Modelling metabolic diseases and drug response using stem cells and organoids

Hu, W., & Lazar, M. A. (2022) · Nature Reviews Endocrinology · doi:10.1038/s41574-022-00733-z · Cited by 103

Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip

Wiedenmann, S., Breunig, M., Merkle, J., von Toerne, C., Georgiev, T., Moussus, M., Schulte, L.-A., Seufferlein, T. T. W., Sterr, M., Lickert, H., et al. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00757-2 · Cited by 102

A Novel Soluble ACE2 Variant with Prolonged Duration of Action Neutralizes SARS-CoV-2 Infection in Human Kidney Organoids

Wysocki, J. A., Ye, M., Hassler, L., Gupta, A., Wang, Y., Nicoleascu, V., Randall, G. C., Wertheim, J. A., & BATLLE, D. C. (2021) · Journal of the American Society of Nephrology · doi:10.1681/asn.2020101537 · Cited by 100

Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo

Tanimizu, N., Ichinohe, N., Sasaki, Y., Itoh, T., Sudo, R., Yamaguchi, T., Katsuda, T., Ninomiya, T., Tokino, T., Ochiya, T., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-23575-1 · Cited by 99

Bile‐Derived Organoids From Patients With Primary Sclerosing Cholangitis Recapitulate Their Inflammatory Immune Profile

Soroka, C. J., Assis, D. N., Alrabadi, L. S., Roberts, S. J., Cusack, L., Jaffe, A., & Boyer, J. L. (2018) · Hepatology · doi:10.1002/hep.30470 · Cited by 97

Helicobacter pylori–induced RASAL2 Through Activation of Nuclear Factor-κB Promotes Gastric Tumorigenesis via β-catenin Signaling Axis

Cao, L., Zhu, S., Lu, H., Soutto, M., Bhat, N. S., Chen, Z., Peng, D., Lin, J., Lu, J. K., Li, P., et al. (2022) · Gastroenterology · doi:10.1053/j.gastro.2022.01.046 · Cited by 95

Drug screening and genome editing in human pancreatic cancer organoids identifies drug-gene interactions and candidates for off-label therapy

Hirt, C., Booij, T. H., Grob, L., Simmler, P., Toussaint, N. C., Keller, D., Taube, D., Ludwig, V., Goryachkin, A., Pauli, C., et al. (2022) · Cell Genomics · doi:10.1016/j.xgen.2022.100095 · Cited by 95

“Proteotranscriptomic analysis of advanced colorectal cancer patient derived organoids for drug sensitivity prediction”

Papaccio, F., García-Micó, B., Gimeno-Valiente, F., Cabeza-Segura, M., Gambardella, V., Gutiérrez-Bravo, M. F., Alfaro‐Cervelló, C., Martínez‐Ciarpaglini, C., Rentero-Garrido, P., Zúñiga, S., et al. (2023) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-022-02591-z · Cited by 93

Design and fabrication of an integrated heart-on-a-chip platform for construction of cardiac tissue from human iPSC-derived cardiomyocytes and in situ evaluation of physiological function

Zhang, F., Qu, K.-Y., Zhou, B., Luo, Y., Zhu, Z., Pan, D., Cui, C., Zhu, Y., Chen, M., & Huang, N. (2021) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2021.113080 · Cited by 92

Single-cell ATAC-seq of fetal human retina and stem-cell-derived retinal organoids shows changing chromatin landscapes during cell fate acquisition

Finkbeiner, C., Ortuño‐Lizarán, I., Sridhar, A., Hooper, M. J., Petter, S., & Reh, T. A. (2022) · Cell Reports · doi:10.1016/j.celrep.2021.110294 · Cited by 92

Generation of the organotypic kidney structure by integrating pluripotent stem cell-derived renal stroma

Tanigawa, S., Tanaka, E., Miike, K., Ohmori, T., Inoue, D., Cai, C., Taguchi, A., Kobayashi, A., & Nishinakamura, R. (2022) · Nature Communications · doi:10.1038/s41467-022-28226-7 · Cited by 92

Development of human pluripotent stem cell-derived hepatic organoids as an alternative model for drug safety assessment

Kim, H., Im, I., Jeon, J. S., Kang, E.-H., Lee, H., Jo, S., Kim, J., Woo, D., Choi, Y. J., Kim, H. J., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121575 · Cited by 91

Black Phosphorus Quantum Dots Cause Nephrotoxicity in Organoids, Mice, and Human Cells

He, C., Ruan, F., Jiang, S., Zeng, J., Yin, H., Liu, R., Zhang, Y., Huang, L., Wang, C., Ma, S., et al. (2020) · Small · doi:10.1002/smll.202001371 · Cited by 90

Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip

Shin, Y. C., Shin, W., Koh, D., Wu, A., Ambrosini, Y. M., Min, S., Eckhardt, S., Fleming, R., Kim, S., Park, S., et al. (2020) · Micromachines · doi:10.3390/mi11070663 · Cited by 89

Clinical application of a lung cancer organoid (tumoroid) culture system

Yokota, E., Iwai, M., Yukawa, T., Yoshida, M., Naomoto, Y., Haisa, M., Monobe, Y., Takigawa, N., Guo, M., Maeda, Y., et al. (2021) · npj Precision Oncology · doi:10.1038/s41698-021-00166-3 · Cited by 89

Functional Characterization of Organoids Derived From Irreversibly Damaged Liver of Patients With NASH

McCarron, S., Bathon, B., Conlon, D. M., Abbey, D., Rader, D. J., Gawronski, K., Brown, C. D., Olthoff, K. M., Shaked, A., & Raabe, T. D. (2021) · Hepatology · doi:10.1002/hep.31857 · Cited by 89

Overriding Adaptive Resistance to Sorafenib Through Combination Therapy With Src Homology 2 Domain–Containing Phosphatase 2 Blockade in Hepatocellular Carcinoma

Leung, C. O. N., Tong, M., Chung, K. P. S., Zhou, L., Che, N., Tang, K. H., Ding, J., Lau, E. Y.-T., Ng, I. O., Ma, S., et al. (2019) · Hepatology · doi:10.1002/hep.30989 · Cited by 88

Squidiff: predicting cellular development and responses to perturbations using a diffusion model

He, S., Zhu, Y., Tavakol, D. N., Ye, H., Lao, Y.-H., Zhu, Z., Xu, C., Chauhan, S., Garty, G., Tomer, R., et al. (2026) · Nature Methods · doi:10.1038/s41592-025-02877-y · Cited by 38

Ex Vivo Tools and Models in MASLD Research

Velliou, R.-I., Giannousi, E., Ralliou, C., Kassi, E., & Chatzigeorgiou, A. (2024) · Cells · doi:10.3390/cells13221827 · Cited by 15