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Cited by 9,711

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample

Open access
Caporaso, J. Gregory, Lauber, Christian L., Walters, William A., Berg-Lyons, Donna, Lozupone, Catherine A., Turnbaugh, Peter J., Fierer, Noah, Knight, Rob·2011·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.1000080107
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Abstract

The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In particular, the coevolution of new sequencing platforms and new software tools allows data acquisition and analysis on an unprecedented scale. Here we report the next stage in this coevolutionary arms race, using the Illumina GAIIx platform to sequence a diverse array of 25 environmental samples and three known “mock communities” at a depth averaging 3.1 million reads per sample. We demonstrate excellent consistency in taxonomic recovery and recapture diversity patterns that were previously reported on the basis of metaanalysis of many studies from the literature (notably, the saline/nonsaline split in environmental samples and the split between host-associated and free-living communities). We also demonstrate that 2,000 Illumina single-end reads are sufficient to recapture the same relationships among samples that we observe with the full dataset. The results thus open up the possibility of conducting large-scale studies analyzing thousands of samples simultaneously to survey microbial communities at an unprecedented spatial and temporal resolution.

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Citations

Cites

Show all 7 references

Research works printed but not linked: 27

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Cited by

Metadata

Authors
Caporaso, J. G., Lauber, C. L., Walters, W. A., Berg-Lyons, D., Lozupone, C. A., Turnbaugh, P. J., Fierer, N., & Knight, R.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2010-06-03
Volume
108
Issue
supplement_1
Pages
4516-4522
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.1000080107
OpenAlex
W2080283023
Cited by
9,711
References
7
FWCI
60.47
Type
journal article
Language
en
Open access
green

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