PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

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Lowry, OliverH., Rosebrough, NiraJ., Farr, A. Lewis, Randall, RoseJ.·1951·Journal of Biological Chemistry

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Abstract

Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins (l), a number of modified analytical procedures ut.ilizing this reagent have been reported for the determination of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and in insulin (10).Although the reagent would seem to be recommended by its great sensitivity and the simplicity of procedure possible with its use, it has not found great favor for general biochemical purposes.In the belief that this reagent, nevertheless, has considerable merit for certain application, but that its peculiarities and limitations need to be understood for its fullest exploitation, it has been studied with regard t.o effects of variations in pH, time of reaction, and concentration of reactants, permissible levels of reagents commonly used in handling proteins, and interfering subst.ances.Procedures are described for measuring protein in solution or after precipitation wit,h acids or other agents, and for the determination of as little as 0.2 y of protein. MethodReagents-Reagent A, 2 per cent N&OX in 0.10 N NaOH.Reagent B, 0.5 per cent CuS04.5Hz0 in 1 per cent sodium or potassium tartrabe.Reagent C, alkaline copper solution.Mix 50 ml. of Reagent A with 1 ml. of Reagent B. Discard after 1 day.Reagent D, carbonate-copper solution, is the same as Reagent C except for omission of NaOH.Reagent E, diluted Folin reagent.Titrate Folin-Ciocalteu phenol reagent ((II), Eimer and Amend, Fisher Scientific Company, New York) with NaOH t.o a phenolphthalein end-point.On the basis of this titration dilute the Folin reagent (about 2-fold) to make it 1 N in acid.Working standards may be prepared from human serum diluted IOO-to lOOO-fold (approximately 700 to 70 y per ml.).These in turn may be checked against a standard solution of crystalline bovine albumin (Armour and * Supported in part by a grant from the American Cancer Society on the recommendation of the Committee on Growth of the National Research Council.

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Wu, 1922, J. Biol. Chem, 51, 33, 10.1016/S0021-9258(18)85898-7 · DOI
Wu, 1927, Chinese J. Physiol, 1, 161
Greenberg, 1929, J. Biol. Chem, 82, 545, 10.1016/S0021-9258(20)78300-6 · DOI
Andersch, 1933, J. Lab. and Clin. Med, 18, 816
Greenberg, 1936, J. Lab. and Clin. Med, 21, 431
Minot, 1936, J. Lab. and Clin. Med, 21, 743
Pressman, 1943, Ind. and Eng. Chem., Anal. Ed, 15, 357, 10.1021/i560117a023 · DOI
Heidelberger, 1943, Science, 97, 405, 10.1126/science.97.2522.405 · DOI
Sutherland, 1949, J. Biol. Chem, 180, 825, 10.1016/S0021-9258(18)56702-8 · DOI
Folin, 1927, J. Biol. Chem, 73, 627, 10.1016/S0021-9258(18)84277-6 · DOI
Lowry, 1946, J. Biol. Chem, 163, 633, 10.1016/S0021-9258(17)41291-9 · DOI
Levy, 1945, Compt.-rend. trav. Lab. Carlsberg, Série chim, 21, 101
Bessey, 1946, J. Biol. Chem, 164, 321, 10.1016/S0021-9258(18)43072-4 · DOI
Bessey, 1946, J. Biol. Chem, 166, 177, 10.1016/S0021-9258(17)34996-7 · DOI
Folin, 1912, J. Biol. Chem, 12, 239, 10.1016/S0021-9258(18)88697-5 · DOI
Herriott, 1935, J. Gen. Physiol, 19, 283, 10.1085/jgp.19.2.283 · DOI
Herriott, 1941, Proc. Soc. Exp. Biol. and Med, 46, 642, 10.3181/00379727-46-12091 · DOI
Mehl, 1949, J. Biol. Chem, 177, 13, 10.1016/S0021-9258(18)57051-4 · DOI
Rising, 1932, J. Biol. Chem, 99, 755, 10.1016/S0021-9258(18)76024-9 · DOI
Hitchings, 1941, J. Biol. Chem, 139, 843, 10.1016/S0021-9258(18)72955-4 · DOI
Funk, 1913, Biochem. J, 7, 356, 10.1042/bj0070356 · DOI
Kunkel, 1950, J. Biol. Chem, 182, 597, 10.1016/S0021-9258(18)56494-2 · DOI
Miller, 1945, J. Biol. Chem, 159, 373, 10.1016/S0021-9258(19)52798-3 · DOI
Weichselbaum, T. E., Am. J. Clin. Path., 16, Tech. Sect., 10, 40 (1946)

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Pulmonary epithelial response in the rat lung to instilled Montserrat respirable dusts and their major mineral components
Housley, D. G., Bérubé, K. A., Jones, T. P., Anderson, S., Pooley, F. D., & Richards, R. J. (2002) · Occupational and Environmental Medicine · doi:10.1136/oem.59.7.466 · Cited by 36
IL-6 increases muscle insulin sensitivity only at superphysiological levels
Geiger, P. C., Hancock, C., Wright, D. C., Han, D.-H., & Holloszy, J. O. (2007) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00701.2006 · Cited by 36
Effects of UVA and visible light on the photogenotoxicity of benzo[a]pyrene and pyrene
Botta, C., Di Giorgio, C., Sabatier, A., & De Méo, M. (2009) · Environmental Toxicology · doi:10.1002/tox.20455 · Cited by 36
Salacia reticulata inhibits differentiation of 3T3-L1 adipocytes
Shimada, T., Nagai, E., Harasawa, Y., Watanabe, M., Negishi, K., Akase, T., Sai, Y., Miyamoto, K.-I., & Aburada, M. (2011) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2011.04.012 · Cited by 36
Untitled work
(1973) · PubMed · Cited by 36
Associations among plasma vitamin C, epidermal ceramide and clinical severity of atopic dermatitis
Shin, J., Kim, Y. J., Kwon, O., Kim, N.-I., & Cho, Y. (2016) · Nutrition Research and Practice · doi:10.4162/nrp.2016.10.4.398 · Cited by 35
Biologically active interleukin 1 in human eccrine sweat: Site-dependent variations in α/β ratios and stress-induced increased excretion
Didierjean, L., Gruaz, D., Frobert, Y., Grassi, J., Dayer, J.-M., & Saurat, J.-H. (1990) · Cytokine · doi:10.1016/1043-4666(90)90053-v · Cited by 35
Gastroprotective effect of kefir on ulcer induced in irradiated rats
Fahmy, H. A., & Ismail, A. F. M. (2015) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/j.jphotobiol.2015.02.009 · Cited by 35
Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson’s Disease
Kakoty, V., K C, S., Dubey, S. K., Yang, C.-H., & Taliyan, R. (2021) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.1c00144 · Cited by 33
Decrease of gene expression of astrocytic 5-HT2B receptors parallels development of depressive phenotype in a mouse model of Parkinson’s disease
Zhang, X., Song, D., Gu, L., Ren, Y., Verkhratsky, A., & Peng, L. (2015) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2015.00388 · Cited by 35
Neuronal degeneration, synaptic defects, and behavioral abnormalities in tau45-230 transgenic mice
Lang, A. E., Riherd Methner, D. N., & Ferreira, A. (2014) · Neuroscience · doi:10.1016/j.neuroscience.2014.06.017 · Cited by 35
Methylphenidate Decreases ATP Levels and Impairs Glutamate Uptake and Na+,K+-ATPase Activity in Juvenile Rat Hippocampus
Schmitz, F., Pierozan, P., Rodrigues, A. F., Biasibetti, H., Grings, M., Zanotto, B., Coelho, D. M., Vargas, C. R., Leipnitz, G., & Wyse, A. T. S. (2017) · Molecular Neurobiology · doi:10.1007/s12035-016-0289-1 · Cited by 35
Comparative studies of aryl hydrocarbon hydroxylase and the Ah receptor in nonmammalian species
Denison, M. S., Hamilton, J. W., & Wilkinson, C. F. (1985) · Comparative Biochemistry and Physiology Part C: Comparative Pharmacology · doi:10.1016/0742-8413(85)90063-5 · Cited by 35
Chronic stress and lithium treatments alter hippocampal glutamate uptake and release in the rat and potentiate necrotic cellular death after oxygen and glucose deprivation
de Vasconcellos-Bittencourt, A. P. S., Vendite, D. A., Nassif, M., Crema, L. M., Frozza, R., Thomazi, A. P., Nieto, F. B., Wofchuk, S., Salbego, C., da Rocha, E. R., et al. (2011) · Neurochemical Research · doi:10.1007/s11064-011-0404-7 · Cited by 36
Human renal mesangial cells are a target for the anti-inflammatory action of 9-cis retinoic acid
Manzano, V. M., Muñoz, J. C. S., Jiménez, J. R., Puyol, M. R., Puyol, D. R., Kitamura, M., & Cazaña, F. J. L. (2000) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0703728 · Cited by 35
Mechanism of ethyl acetate synthesis by Kluyveromyces fragilis
Kallel-Mhiri, H., & Miclo, A. (1993) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1993.tb06387.x · Cited by 34
The cardioprotective compound cloxyquin uncouples mitochondria and induces autophagy
Zhang, J., Nadtochiy, S. M., Urciuoli, W. R., & Brookes, P. S. (2016) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.00926.2014 · Cited by 36
Aluminum-induced molecular neurodegeneration: The protective role of genistein and chickpea extract
Wahby, M. M., Mohammed, D. S., Newairy, A. A., Abdou, H. M., & Zaky, A. (2017) · Food and Chemical Toxicology · doi:10.1016/j.fct.2017.05.044 · Cited by 35
Postnatal exposure to poly (I:C) impairs learning and memory through changes in synaptic plasticity gene expression in developing rat brain
Baghel, M. S., Singh, B., Dhuriya, Y. K., Shukla, R. K., Patro, N., Khanna, V. K., Patro, I. K., & Thakur, M. K. (2018) · Neurobiology of Learning and Memory · doi:10.1016/j.nlm.2018.09.005 · Cited by 35
Effects of gender and gonadectomy on responses to chronic benzodiazepine receptor agonist exposure in rats
Wilson, M. A., & Biscardi, R. (1992) · European Journal of Pharmacology · doi:10.1016/0014-2999(92)90614-a · Cited by 34
Efficacy of the combination of N-acetylcysteine and desferrioxamine in the prevention and treatment of gentamicin-induced acute renal failure in male Wistar rats
Petronilho, F., Constantino, L., de Souza, B., Reinke, A., Martins, M. R., Fraga, C. M., Ritter, C., & Dal-Pizzol, F. (2009) · Nephrology Dialysis Transplantation · doi:10.1093/ndt/gfn774 · Cited by 34
Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803
Hagemann, M., Gärtner, K., Scharnagl, M., Bolay, P., Lott, S. C., Fuss, J., Huettel, B., Reinhardt, R., Klähn, S., & Hess, W. R. (2018) · DNA Research · doi:10.1093/dnares/dsy006 · Cited by 34
Antioxidant intervention attenuates oxidative stress in children and teenagers with Down syndrome
Parisotto, E. B., Garlet, T. R., Cavalli, V. L. D. L. O., Zamoner, A., da Rosa, J. S., Bastos, J., Micke, G. A., Fröde, T. S., Pedrosa, R. C., & Wilhelm Filho, D. (2014) · Research in Developmental Disabilities · doi:10.1016/j.ridd.2014.03.013 · Cited by 34
Endogenous C19-steroids and oestradiol levels in human primary breast tumour tissues and their correlation with androgen and oestrogen receptors
Mistry, P., Griffiths, K., & Maynard, P. V. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90372-9 · Cited by 34
Leptin Deficiency Per Se Dictates Body Composition and Insulin Action in ob/ob Mice
Van Den Hoek, A. M., Teusink, B., Voshol, P. J., Havekes, L. M., Romijn, J. A., & Pijl, H. (2008) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2007.01626.x · Cited by 34
Attenuation of oxidative stress, inflammation and early markers of tumor promotion by caffeic acid in Fe-NTA exposed kidneys of Wistar rats
U. Rehman, M., & Sultana, S. (2011) · Molecular and Cellular Biochemistry · doi:10.1007/s11010-011-0881-7 · Cited by 34
High-rate CO 2 sequestration using a novel venturi integrated photobioreactor and subsequent valorization to microalgal lipids
Dey, S., Bhattacharya, A., Kumar, P., & Malik, A. (2020) · Green Chemistry · doi:10.1039/d0gc02552f · Cited by 35
Alterations in Rat Lipid Metabolism Following Ecdysterone Treatment
Catalán, R. E., Martinez, A. M., Aragones, M. D., Miguel, B. G., Robles, A., & Godoy, J. E. (1985) · Comparative Biochemistry and Physiology Part B Comparative Biochemistry · doi:10.1016/0305-0491(85)90403-1 · Cited by 34
Exopolysaccharide-Producing Mesophilic Lactic Cultures for Preparation of Fat-Free Dahi-an Indian Fermented Milk
Behare, P., Singh, R., & Singh, R. P. (2009) · Journal of Dairy Research · doi:10.1017/s0022029908003865 · Cited by 34
ISOLATION AND SOME CHARACTERISTICS OF CELL NUCLEI FROM BRAIN CORTEX OF ADULT CAT
Hadjiolov, A. A., Tencheva, Z. S., & Bojadjieva-Mikhailova, A. G. (1965) · The Journal of Cell Biology · doi:10.1083/jcb.26.2.383 · Cited by 34
Mechanism of abnormal production of adrenal androgens in patients with adrenocortical adenomas and carcinomas.
Sakai, Y., Yanase, T., Hara, T., Takayanagi, R., Haji, M., & Nawata, H. (1994) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.78.1.8288710 · Cited by 34
Thiols as potential UV radiation protectors: An in vitro study
van den Broeke, L. T., & van Henegouwen, G. M. J. B. (1993) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/1011-1344(93)80026-6 · Cited by 34
Presence of dolichol and its derivatives in human blood.
Elmberger, P. G., Engfeldt, P., & Dallner, G. (1988) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38414-5 · Cited by 34

Authors: Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R.
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1951-11
Volume: 193
Issue: 1
Pages: 265-275
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1016/s0021-9258(19)52451-6
OpenAlex: W1775749144
Cited by: 317,331
References: 0
FWCI: 30.86
Type: journal article
Language: en
Open access: hybrid
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 25178 d)

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.

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