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Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells

Open access
Mittelbrunn, María, Gutiérrez-Vázquez, Cristina, Villarroya-Beltri, Carolina, González, Susana, Sánchez-Cabo, Fátima, González, Manuel Ángel, Bernad, Antonio, Sánchez-Madrid, Francisco·2011·Nature Communications
DOI 10.1038/ncomms1285
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Abstract

The immune synapse is an exquisitely evolved means of communication between T cells and antigen-presenting cells (APCs) during antigen recognition. Recent evidence points to the transfer of RNA via exosomes as a novel mode of intercellular communication. Here we show that exosomes of T, B and dendritic immune cells contain microRNA (miRNA) repertoires that differ from those of their parent cells. We investigate whether miRNAs are exchanged during cognate immune interactions, and demonstrate the existence of antigen-driven unidirectional transfer of miRNAs from the T cell to the APC, mediated by the delivery of CD63+ exosomes on immune synapse formation. Inhibition of exosome production by targeting neutral sphingomyelinase-2 impairs transfer of miRNAs to APCs. Moreover, miRNAs transferred during immune synapsis are able to modulate gene expression in recipient cells. Thus, our results support a mechanism of cellular communication involving antigen-dependent, unidirectional intercellular transfer of miRNAs by exosomes during immune synapsis. Exosomes released from cells can transfer RNA to recipient cells. In this study, the authors demonstrate that microRNAs in exosomes from T cells can be transferred to antigen-presenting cells during immune synapsis, and that this can alter gene expression, suggesting a new form of cellular communication.

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Cited by

Metadata

Authors
Mittelbrunn, M., Gutiérrez-Vázquez, C., Villarroya-Beltri, C., González, S., Sánchez-Cabo, F., González, M. Á., Bernad, A., & Sánchez-Madrid, F.
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2011-04-19
Volume
2
Issue
1
Pages
282-282
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms1285
OpenAlex
W1974035668
Cited by
1,769
References
10
FWCI
37.15
Type
journal article
Language
en
Open access
hybrid
Licence

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