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Optogenetics: 10 years of microbial opsins in neuroscience

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Deisseroth, Karl·2015·Nature Neuroscience
DOI 10.1038/nn.4091
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Abstract

Over the past 10 years, the development and convergence of microbial opsin engineering, modular genetic methods for cell-type targeting and optical strategies for guiding light through tissue have enabled versatile optical control of defined cells in living systems, defining modern optogenetics. Despite widespread recognition of the importance of spatiotemporally precise causal control over cellular signaling, for nearly the first half (2005–2009) of this 10-year period, as optogenetics was being created, there were difficulties in implementation, few publications and limited biological findings. In contrast, the ensuing years have witnessed a substantial acceleration in the application domain, with the publication of thousands of discoveries and insights into the function of nervous systems and beyond. This Historical Commentary reflects on the scientific landscape of this decade-long transition.

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Show all 36 references

Research works printed but not linked: 164

  1. Deisseroth, K. Nat. Methods 8, 26–29 (2011). · DOI · Paper page
  2. Boyden, E.S., Zhang, F., Bamberg, E., Nagel, G. & Deisseroth, K. Nat. Neurosci. 8, 1263–1268 (2005). · DOI · Paper page
  3. Deisseroth, K. et al. J. Neurosci. 26, 10380–10386 (2006). · DOI · Paper page
  4. Grosenick, L., Marshel, J.H. & Deisseroth, K. Neuron 86, 106–139 (2015). · DOI · Paper page
  5. Zhang, F. et al. Cell 147, 1446–1457 (2011). · DOI · Paper page
  6. Stryer, L. Proc. Natl. Acad. Sci. USA 93, 557–559 (1996). · DOI
  7. Oesterhelt, D. & Stoeckenius, W. Nat. New Biol. 233, 149–152 (1971). · DOI · Paper page
  8. Stryer, L. Biochemistry 3rd edn. 962 (Freeman, 1988).
  9. Kato, H.E. et al. Nature 482, 369–374 (2012). · DOI · Paper page
  10. Berndt, A., Lee, S.Y., Ramakrishnan, C. & Deisseroth, K. Science 344, 420–424 (2014). · DOI · Paper page
  11. Wietek, J. et al. Science 344, 409–412 (2014). · DOI · Paper page
  12. Govorunova, E.G., Sineshchekov, O.A., Janz, R., Liu, X. & Spudich, J.L. Science 349, 647–650 (2015). · DOI · Paper page
  13. Berndt, A. & Deisseroth, K. Science 349, 590–591 (2015). · DOI
  14. Hoffmann, A., Hildebrandt, V., Heberle, J. & Büldt, G. Proc. Natl. Acad. Sci. USA 91, 9367–9371 (1994). · DOI
  15. Nagel, G. et al. Science 296, 2395–2398 (2002). · DOI · Paper page
  16. Nagel, G. et al. Proc. Natl. Acad. Sci. USA 100, 13940–13945 (2003). · DOI · Paper page
  17. Khorana, H.G., Knox, B.E., Nasi, E., Swanson, R. & Thompson, D.A. Proc. Natl. Acad. Sci. USA 85, 7917–7921 (1988). · DOI
  18. Gradinaru, V. et al. Cell 141, 154–165 (2010). · DOI · Paper page
  19. Zemelman, B.V., Lee, G.A., Ng, M. & Miesenböck, G. Neuron 33, 15–22 (2002). · DOI · Paper page
  20. Zemelman, B.V., Nesnas, N., Lee, G.A. & Miesenbock, G. Proc. Natl. Acad. Sci. USA 100, 1352–1357 (2003). · DOI · Paper page
  21. Banghart, M., Borges, K., Isacoff, E., Trauner, D. & Kramer, R.H. Nat. Neurosci. 7, 1381–1386 (2004). · DOI · Paper page
  22. Lima, S.Q. & Miesenböck, G. Cell 121, 141–152 (2005). · DOI · Paper page
  23. Volgraf, M. et al. Nat. Chem. Biol. 2, 47–52 (2006). · DOI · Paper page
  24. Zhang, F., Wang, L.-P., Boyden, E.S. & Deisseroth, K. Nat. Methods 3, 785–792 (2006). · DOI · Paper page
  25. Aravanis, A.M. et al. J. Neural Eng. 4, S143–S156 (2007). · DOI · Paper page
  26. Li, X. et al. Proc. Natl. Acad. Sci. USA 102, 17816–17821 (2005). · DOI · Paper page
  27. Nagel, G. et al. Curr. Biol. 15, 2279–2284 (2005). · DOI · Paper page
  28. Ishizuka, T., Kakuda, M., Araki, R. & Yawo, H. Neurosci. Res. 54, 85–94 (2006). · DOI · Paper page
  29. Bi, A. et al. Neuron 50, 23–33 (2006). · DOI · Paper page
  30. Schroll, C. et al. Curr. Biol. 16, 1741–1747 (2006). · DOI · Paper page
  31. Hwang, R.Y. et al. Curr. Biol. 17, 2105–2116 (2007). · DOI · Paper page
  32. Zhang, F. et al. Nature 446, 633–639 (2007). · DOI · Paper page
  33. Wang, H. et al. Proc. Natl. Acad. Sci. USA 104, 8143–8148 (2007). · DOI · Paper page
  34. Arenkiel, B.R. et al. Neuron 54, 205–218 (2007). · DOI · Paper page
  35. Gradinaru, V. et al. J. Neurosci. 27, 14231–14238 (2007). · DOI · Paper page
  36. Zhang, F. in Larry Katz Memorial Lecture, Cold Spring Harbor Laboratory Meeting on Neuronal Circuits (Cold Spring Harbor Laboratory Press, 2008).
  37. Atasoy, D., Aponte, Y., Su, H.H. & Sternson, S.M.A. J. Neurosci. 28, 7025–7030 (2008). · DOI · Paper page
  38. Hägglund, M., Borgius, L., Dougherty, K.J. & Kiehn, O. Nat. Neurosci. 13, 246–252 (2010). · DOI · Paper page
  39. Douglass, A.D. et al. Curr. Biol. 18, 1133–1137 (2008). · DOI · Paper page
  40. Gradinaru, V., Mogri, M., Thompson, K.R., Henderson, J.M. & Deisseroth, K. Science 324, 354–359 (2009). · DOI · Paper page
  41. Harz, H. & Hegemann, P. Nature 351, 489–491 (1991). · DOI · Paper page
  42. Zhang, F. et al. Nat. Neurosci. 11, 631–633 (2008). · DOI · Paper page
  43. Berndt, A., Yizhar, O., Gunaydin, L.A., Hegemann, P. & Deisseroth, K. Nat. Neurosci. 12, 229–234 (2009). · DOI · Paper page
  44. Bamann, C., Gueta, R., Kleinlogel, S., Nagel, G. & Bamberg, E. Biochemistry 49, 267–278 (2010). · DOI
  45. Gradinaru, V., Thompson, K.R. & Deisseroth, K. Brain Cell Biol. 36, 129–139 (2008). · DOI · Paper page
  46. Zhao, S. et al. Brain Cell Biol. 36, 141–154 (2008). · DOI
  47. Wang, H. et al. J. Biol. Chem. 284, 5685–5696 (2009). · DOI · Paper page
  48. Lin, J.Y., Lin, M.Z., Steinbach, P. & Tsien, R.Y. Biophys. J. 96, 1803–1814 (2009). · DOI · Paper page
  49. Fenno, L.E. et al. Nat. Methods 11, 763–772 (2014). · DOI · Paper page
  50. Flavell, S.W. et al. Cell 154, 1023–1035 (2013). · DOI · Paper page
  51. Ramdya, P. et al. Nature 519, 233–236 (2015). · DOI
  52. Roberts, T.F., Gobes, S.M.H., Murugan, M., Ölveczky, B.P. & Mooney, R. Nat. Neurosci. 15, 1454–1459 (2012). · DOI
  53. Thiele, T.R., Donovan, J.C. & Baier, H. Neuron 83, 679–691 (2014). · DOI · Paper page
  54. Lu, Y. et al. J. Neurophysiol. 113, 3574–3587 (2015). · DOI
  55. Jorgenson, L.A. et al. Phil. Trans. R. Soc. Lond. B doi:10.1098/rstb.2014.0164 (30 March 2015). · DOI · Paper page
  56. Proville, R.D. et al. Nat. Neurosci. 17, 1233–1239 (2014). · DOI · Paper page
  57. Domingos, A.I. et al. Nat. Neurosci. 14, 1562–1568 (2011). · DOI · Paper page
  58. Yang, Y., Atasoy, D., Su, H.H. & Sternson, S.M. Cell 146, 992–1003 (2011). · DOI · Paper page
  59. Oka, Y., Ye, M. & Zuker, C.S. Nature 520, 349–352 (2015). · DOI
  60. Jennings, J.H. et al. Cell 160, 516–527 (2015). · DOI · Paper page
  61. Gourine, A.V. et al. Science 329, 571–575 (2010). · DOI · Paper page
  62. Abbott, S.B.G., Stornetta, R.L., Coates, M.B. & Guyenet, P.G. J. Neurosci. 31, 16410–16422 (2011). · DOI
  63. Carter, M.E. et al. Nat. Neurosci. 13, 1526–1533 (2010). · DOI · Paper page
  64. Jego, S. et al. Nat. Neurosci. 16, 1637–1643 (2013). · DOI · Paper page
  65. Anaclet, C. et al. Nat. Neurosci. 17, 1217–1224 (2014). · DOI · Paper page
  66. Jones, J.R., Tackenberg, M.C. & McMahon, D.G. Nat. Neurosci. 18, 373–375 (2015). · DOI
  67. Smear, M., Shusterman, R., O'Connor, R., Bozza, T. & Rinberg, D. Nature 479, 397–400 (2011). · DOI · Paper page
  68. Choi, G.B. et al. Cell 146, 1004–1015 (2011). · DOI
  69. Lepousez, G. & Lledo, P.-M. Neuron 80, 1010–1024 (2013). · DOI
  70. Znamenskiy, P. & Zador, A.M. Nature 497, 482–485 (2013). · DOI · Paper page
  71. Zhao, X., Liu, M. & Cang, J. Neuron 84, 202–213 (2014). · DOI
  72. Maksimovic, S. et al. Nature 509, 617–621 (2014). · DOI · Paper page
  73. Kress, G.J. et al. Nat. Neurosci. 16, 665–667 (2013). · DOI · Paper page
  74. Hunnicutt, B.J. et al. Nat. Neurosci. 17, 1276–1285 (2014). · DOI
  75. Lee, J.H. et al. Nature 465, 788–792 (2010). · DOI · Paper page
  76. Thanos, P.K. et al. J. Neurosci. 33, 6343–6349 (2013). · DOI
  77. Pastoll, H., Solanka, L., van Rossum, M.W. & Nolan, M.F. Neuron 77, 141–154 (2013). · DOI · Paper page
  78. Beltramo, R. et al. Nat. Neurosci. 16, 227–234 (2013). · DOI · Paper page
  79. Stark, E. et al. Neuron 83, 467–480 (2014). · DOI · Paper page
  80. Siegle, J.H., Pritchett, D.L. & Moore, C.I. Nat. Neurosci. 17, 1371–1379 (2014). · DOI
  81. Barthó, P. et al. Neuron 82, 1367–1379 (2014). · DOI
  82. Kim, T. et al. Proc. Natl. Acad. Sci. USA 112, 3535–3540 (2015). · DOI · Paper page
  83. Siegle, J. H. & Wilson, M. A. Elife 3, e03061 (2014). · DOI · Paper page
  84. Canty, A.J. et al. J. Neurosci. 29, 10695–10705 (2009). · DOI
  85. Pfeffer, C.K., Xue, M., He, M., Huang, Z.J. & Scanziani, M. Nat. Neurosci. 16, 1068–1076 (2013). · DOI · Paper page
  86. Jackson, J. et al. Nat. Neurosci. 17, 1362–1370 (2014). · DOI
  87. Kohara, K. et al. Nat. Neurosci. 17, 269–279 (2014). · DOI · Paper page
  88. Poulet, J.F.A., Fernandez, L.M.J., Crochet, S. & Petersen, C.C.H. Nat. Neurosci. 15, 370–372 (2012). · DOI · Paper page
  89. Basu, J. et al. Neuron 79, 1208–1221 (2013). · DOI · Paper page
  90. Lien, A.D. & Scanziani, M. Nat. Neurosci. 16, 1315–1323 (2013). · DOI · Paper page
  91. Markopoulos, F., Rokni, D., Gire, D.H. & Murthy, V.N. Neuron 76, 1175–1188 (2012). · DOI · Paper page
  92. Gentet, L.J. et al. Nat. Neurosci. 15, 607–612 (2012). · DOI · Paper page
  93. Gunaydin, L.A. et al. Cell 157, 1535–1551 (2014). · DOI · Paper page
  94. Wu, Z., Autry, A.E., Bergan, J.F., Watabe-Uchida, M. & Dulac, C.G. Nature 509, 325–330 (2014). · DOI · Paper page
  95. Lee, H. et al. Nature 509, 627–632 (2014). · DOI · Paper page
  96. Kunwar, P.S. et al. Elife 4, (2015).10.7554/eLife.06633 · DOI · Paper page
  97. Wang, L., Chen, I.Z. & Lin, D. Neuron 85, 1344–1358 (2015). · DOI · Paper page
  98. Witten, I.B. et al. Science 330, 1677–1681 (2010). · DOI · Paper page
  99. Lobo, M.K. et al. Science 330, 385–390 (2010). · DOI · Paper page
  100. Witten, I.B. et al. Neuron 72, 721–733 (2011). · DOI · Paper page
  101. Steinberg, E.E. et al. Nat. Neurosci. 16, 966–973 (2013). · DOI · Paper page
  102. McDevitt, R.A. et al. Cell Reports 8, 1857–1869 (2014). · DOI · Paper page
  103. Kim, S.-Y. et al. Nature 496, 219–223 (2013). · DOI · Paper page
  104. Felix-Ortiz, A.C. et al. Neuron 79, 658–664 (2013). · DOI · Paper page
  105. Wolff, S.B.E. et al. Nature 509, 453–458 (2014). · DOI · Paper page
  106. Amo, R. et al. Neuron 84, 1034–1048 (2014). · DOI · Paper page
  107. Deisseroth, K. Nature 505, 309–317 (2014). · DOI
  108. Goshen, I. et al. Cell 147, 678–689 (2011). · DOI · Paper page
  109. Liu, X. et al. Nature 484, 381–385 (2012). · DOI · Paper page
  110. Xu, W. & Südhof, T.C. Science 339, 1290–1295 (2013). · DOI · Paper page
  111. Cowansage, K.K. et al. Neuron 84, 432–441 (2014). · DOI · Paper page
  112. Brown, M.T.C. et al. Nature 492, 452–456 (2012). · DOI · Paper page
  113. Zhang, S.-J. et al. Science 340, 1232627 (2013). · DOI · Paper page
  114. Wu, Y.-W. et al. Cell Reports 10, 75–87 (2015). · DOI
  115. Larsen, R.S. et al. Neuron 83, 879–893 (2014). · DOI
  116. Jensen, M. et al. Cell 149, 173–187 (2012). · DOI
  117. MacAskill, A.F., Cassel, J.M. & Carter, A.G. Nat. Neurosci. 17, 1198–1207 (2014). · DOI
  118. Suárez, R. et al. Neuron 82, 1289–1298 (2014). · DOI
  119. Duan, X., Krishnaswamy, A., De la Huerta, I. & Sanes, J.R. Cell 158, 793–807 (2014). · DOI
  120. Gibson, E.M. et al. Science 344, 1252304 (2014). · DOI · Paper page
  121. Song, J. et al. Nature 489, 150–154 (2012). · DOI · Paper page
  122. Temprana, S.G. et al. Neuron 85, 116–130 (2015). · DOI · Paper page
  123. Ledri, M. et al. Eur. J. Neurosci. 36, 1971–1983 (2012). · DOI
  124. Paz, J.T. et al. Nat. Neurosci. 16, 64–70 (2013). · DOI · Paper page
  125. Krook-Magnuson, E., Armstrong, C., Oijala, M. & Soltesz, I. Nat. Commun. 4, 1376 (2013). · DOI · Paper page
  126. Iyer, S.M. et al. Nat. Biotechnol. 32, 274–278 (2014). · DOI
  127. Steinbeck, J.A. et al. Nat. Biotechnol. 33, 204–209 (2015). · DOI · Paper page
  128. Suberbielle, E. et al. Nat. Neurosci. 16, 613–621 (2013). · DOI · Paper page
  129. Yamamoto, K. et al. Cell Reports 11, 859–865 (2015). · DOI
  130. Venkatesh, H.S. et al. Cell 161, 803–816 (2015). · DOI · Paper page
  131. Cepeda, C. et al. J. Neurosci. 33, 7393–7406 (2013). · DOI
  132. Busskamp, V. et al. Science 329, 413–417 (2010). · DOI · Paper page
  133. Lim, D.H., LeDue, J.M., Mohajerani, M.H. & Murphy, T.H. J. Neurosci. 34, 16455–16466 (2014). · DOI
  134. Cheng, M.Y. et al. Proc. Natl. Acad. Sci. USA 111, 12913–12918 (2014). · DOI
  135. Chen, B.T. et al. Nature 496, 359–362 (2013). · DOI · Paper page
  136. Lucantonio, F. et al. Nat. Neurosci. 17, 1092–1099 (2014). · DOI
  137. Creed, M., Pascoli, V.J. & Lüscher, C. Science 347, 659–664 (2015). · DOI · Paper page
  138. Burguière, E., Monteiro, P., Feng, G. & Graybiel, A.M. Science 340, 1243–1246 (2013). · DOI · Paper page
  139. Yizhar, O., Fenno, L., Davidson, T., Mogri, M. & Deisseroth, K. Neuron 71, 9–34 (2011). · DOI · Paper page
  140. Prakash, R. et al. Nat. Methods 9, 1171–1179 (2012). · DOI · Paper page
  141. Packer, A.M. et al. Nat. Methods 9, 1202–1205 (2012). · DOI · Paper page
  142. Rickgauer, J.P. et al. Nat. Neurosci. 17, 1816–1824 (2014). · DOI · Paper page
  143. Packer, A.M., Russell, L.E., Dalgleish, H.W. & Häusser, M. Nat. Methods 12, 140–146 (2015). · DOI · Paper page
  144. Mutoh, H., Akemann, W. & Knöpfel, T. ACS Chem. Neurosci. 3, 585–592 (2012). · DOI
  145. Buzsáki, G. et al. Neuron 86, 92–105 (2015). · DOI · Paper page
  146. Cosentino, C. et al. Science 348, 707–710 (2015). · DOI
  147. Airan, R.D., Thompson, K.R., Fenno, L.E., Bernstein, H. & Deisseroth, K. Nature 458, 1025–1029 (2009). · DOI · Paper page
  148. Kim, J.M. et al. Biochemistry 44, 2284–2292 (2005). · DOI
  149. Barish, P.A. et al. Eur. J. Pharmacol. 705, 42–48 (2013). · DOI
  150. Siuda, E.R. et al. Neuron 86, 923–935 (2015). · DOI
  151. Li, P. et al. Mol. Psychiatry doi:10.1038/mp.2014.182 (17 February 2015). · DOI
  152. Oh, E., Maejima, T., Liu, C., Deneris, E. & Herlitze, S. J. Biol. Chem. 285, 30825–30836 (2010). · DOI
  153. van Wyk, M. et al. PLoS Biol. 13, e1002143 (2015). · DOI
  154. Urban, D.J. & Roth, B.L. Annu. Rev. Pharmacol. Toxicol. 55, 399–417 (2015). · DOI · Paper page
  155. Wu, Y.I. et al. Nature 461, 104–108 (2009). · DOI · Paper page
  156. Stierl, M. et al. J. Biol. Chem. 286, 1181–1188 (2011). · DOI · Paper page
  157. Kim, T., Folcher, M., Baba, M.D.-E. & Fussenegger, M. Angew. Chem. Int. Edn. Engl. 54, 5933–5938 (2015). · DOI
  158. Arrenberg, A.B., Stainier, D.Y.R., Baier, H. & Huisken, J. Science 330, 971–974 (2010). · DOI · Paper page
  159. Chen, R., Romero, G., Christiansen, M.G., Mohr, A. & Anikeeva, P. Science 347, 1477–1480 (2015). · DOI · Paper page
  160. Carvalho-de-Souza, J.L. et al. Neuron 86, 207–217 (2015). · DOI · Paper page
  161. Tochitsky, I. et al. Neuron 81, 800–813 (2014). · DOI
  162. Matsuno-Yagi, A. & Mukohata, Y. Biochem. Biophys. Res. Commun. 78, 237–243 (1977). · DOI · Paper page
  163. Schobert, B. & Lanyi, J.K. J. Biol. Chem. 257, 10306–10313 (1982). · DOI · Paper page
  164. Foster, K.W. et al. Nature 311, 756–759 (1984). · DOI · Paper page

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Metadata

Authors
Deisseroth, K.
Venue
Nature Neuroscience
Publisher
Springer Science and Business Media LLC
Published
2015-08-26
Volume
18
Issue
9
Pages
1213-1225
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
DOI
10.1038/nn.4091
OpenAlex
W1139015881
Cited by
1,283
References
36
FWCI
48.11
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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