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Cited by 10,405

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

Tuerk, Craig, Gold, Larry·1990·Science
DOI 10.1126/science.2200121
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Abstract

High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule.

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Research works printed but not linked: 32

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Cited by

Metadata

Authors
Tuerk, C., & Gold, L.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1990-08-03
Volume
249
Issue
4968
Pages
505-510
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.2200121
OpenAlex
W2079321730
Cited by
10,405
References
2
FWCI
15.53
Type
journal article
Language
en
Open access
closed

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